(A–D) Representative images of iWAT from (A–B) control and (C–D) cPrdm16KO mice at P6, immunolabeled with TH. MIPs from 50 μm z-stacks are shown. (E–H) Overlay of TH (green) and UCP1 (magenta), corresponding to (A–D). (A, C, E, and G) Images of the dorsolumbar region. (B, D, F, and H) Images of the inguinal region. Scale bars are indicated. Imaging was performed in at least three independent animals per genotype, and representative images are shown. (I) Normalized gene expression of dorsolumbar vs. inguinal regions in iWAT from control and cPrdm16KO mice at P6, P14, and P21, n = 2–3. Representative genes involved in the adipogenic program are shown. Data are presented as mean ± SEM and analyzed by two-way ANOVA followed by Bonferroni’s multiple comparisons test.