Non-genetic inheritance restraint of cell-to-cell variation

  1. Harsh Vashistha
  2. Maryam Kohram
  3. Hanna Salman  Is a corresponding author
  1. University of Pittsburgh, United States

Abstract

Heterogeneity in physical and functional characteristics of cells (e.g. size, cycle time, growth rate, protein concentration) proliferates within an isogenic population due to stochasticity in intracellular biochemical processes and in the distribution of resources during divisions. Conversely, it is limited in part by the inheritance of cellular components between consecutive generations. Here we introduce a new experimental method for measuring proliferation of heterogeneity in bacterial cell characteristics, based on measuring how two sister cells become different from each other over time. Our measurements provide the inheritance dynamics of different cellular properties, and the 'inertia' of cells to maintain these properties along time. We find that inheritance dynamics are property-specific, and can exhibit long-term memory (~10 generations) that works to restrain variation among cells. Our results can reveal mechanisms of non-genetic inheritance in bacteria and help understand how cells control their properties and heterogeneity within isogenic cell populations.

Data availability

All data generated or analyzed during this study, are available on Zenodo at 10.5281/zenodo.4476617

Article and author information

Author details

  1. Harsh Vashistha

    Physics and Astronomy, University of Pittsburgh, Pittsburgh, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Maryam Kohram

    Physics and Astronomy, University of Pittsburgh, Pittsburgh, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Hanna Salman

    Physics and Astronomy, University of Pittsburgh, Pittsburgh, United States
    For correspondence
    hsalman@pitt.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5847-524X

Funding

United States-Israel Binational Science Foundation (2016376)

  • Hanna Salman

National Science Foundation (2014116)

  • Hanna Salman

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Petra Anne Levin, Washington University in St. Louis, United States

Publication history

  1. Received: November 10, 2020
  2. Accepted: January 28, 2021
  3. Accepted Manuscript published: February 1, 2021 (version 1)
  4. Version of Record published: March 4, 2021 (version 2)

Copyright

© 2021, Vashistha et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Harsh Vashistha
  2. Maryam Kohram
  3. Hanna Salman
(2021)
Non-genetic inheritance restraint of cell-to-cell variation
eLife 10:e64779.
https://doi.org/10.7554/eLife.64779

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