SABRE populates ER domains essential for cell plate maturation and cell expansion influencing cell and tissue patterning

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Abstract

SABRE, which is found throughout eukaryotes and was originally identified in plants, mediates cell expansion, division plane orientation and planar polarity in plants. How and where SABRE mediates these processes remain open questions. We deleted SABRE in Physcomitrium patens, an excellent model for cell biology. SABRE null mutants were stunted, similar to phenotypes in seed plants. Additionally, polarized growing cells were delayed in cytokinesis, sometimes resulting in catastrophic failures. A functional SABRE fluorescent fusion protein localized to dynamic puncta on regions of the ER during interphase and at the cell plate during cell division. Without SABRE, cells accumulated ER aggregates and the ER abnormally buckled along the developing cell plate. Notably, callose deposition was delayed in Δsabre, and in cells that failed to divide, abnormal callose accumulations formed at the cell plate. Our findings revealed a surprising and fundamental role for the ER in cell plate maturation.

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All data generated or analyzed during this study are included in the manuscript and supporting files.

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Xiaohang Cheng

Department of Biological Sciences, Dartmouth College, Hanover, United States

Competing interests
The authors declare that no competing interests exist.
Magdalena Bezanilla

Department of Biological Sciences, Dartmouth College, Hanover, United States

For correspondence
magdalena.bezanilla@dartmouth.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-6124-9916

Funding

National Science Foundation (MCB-1715785)

Magdalena Bezanilla

Dartmouth College (John H. Copenhaver Jr. and William H. Thomas MD 1952 Award)

Xiaohang Cheng

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.