(A) Representative confocal micrographs of cells having the LacO array integrated at a locus of interest, expressing GFP-LacI and Pho88-mCherry (Robinett et al., 1996; Brickner and Walter, 2004; Brickner et al., 2019) and scored as either nucleoplasmic (left) or peripheral (right). (B) Peripheral localization (% of cells ± SEM) of URA3 and HIS4 in cells grown ± histidine. The hatched blue line, here and throughout: peripheral localization predicted by chance. (C, E–G) Kymographs of 10 cells with a LacO array integrated at HIS4 (C), URA3 (E), INO1 (F), or URA3:GRS1 (G) were grown in the indicated medium and scored for peripheral localization every 10 s for 5 min. Yellow: peripheral; purple: nucleoplasmic. (D) Peripheral localization (± SEM) of URA3, INO1, URA3:INO1, and URA3:GRS1 in cells grown ± inositol. (H–J) Summary plots from (C, E–G): (H) mean percentage of cells (± SD) in which the locus is peripheral at each time point (i.e., each dot represents a summary of a single column from kymographs); (I) mean percentage of time (± SD) each locus spent colocalized with the nuclear envelope (i.e., each dot represents a summary of a single row from kymographs); and (J) the distribution and median duration of periods of peripheral localization of each locus.