Random sub-diffusion and capture of genes by the nuclear pore reduces dynamics and coordinates interchromosomal movement
Abstract
Hundreds of genes interact with the yeast nuclear pore complex (NPC), localizing at the nuclear periphery and clustering with co-regulated genes. Dynamic tracking of peripheral genes shows that they cycle on and off the NPC and that interaction with the NPC slows their sub-diffusive movement. Furthermore, NPC-dependent inter-chromosomal clustering leads to coordinated movement of pairs of loci separated by hundreds of nanometers. We developed Fractional Brownian Motion simulations for chromosomal loci in the nucleoplasm and interacting with NPCs. These simulations predict the rate and nature of random sub-diffusion during repositioning from nucleoplasm to periphery and match measurements from two different experimental models, arguing that recruitment to the nuclear periphery is due to random sub-diffusion and transient capture by NPCs. Finally, the simulations do not lead to inter-chromosomal clustering or coordinated movement, suggesting that interaction with the NPC is necessary, but not sufficient, to cause clustering.
Data availability
All tracking data will be included as Source Data. All Scripts are publicly available from Github (https://github.com/MCnu/R_sim_scripts).
Article and author information
Author details
Funding
National Institutes of Health (R01 GM118712)
- Michael Chas Sumner
- Donna G Brickner
- Jason H Brickner
National Institutes of Health (R35 GM136419)
- Michael Chas Sumner
- Donna G Brickner
- Jason H Brickner
National Cancer Institute (U54 CA193419)
- Michael Chas Sumner
- Jason H Brickner
National Institutes of Health (T32 GM008061)
- Michael Chas Sumner
Department of Energy, Labor and Economic Growth (DE-FG02-97ER25308)
- Steven B Torrisi
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2021, Sumner et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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