Cell line (Homo sapiens) | HAP1 | Whitehead Institute | Cellosaurus: CVCL_Y019 | kind gift from Thijn Brummelkamp (NKI) |
Cell line (Homo sapiens) | HAP1SGFP2-CTNNB1 | This paper | | |
Cell line (Homo sapiens) | HAP1SGFP2-CTNNB1(S45F) | This paper | | |
Transfected construct (Homo sapiens) | pSpCas9(BB)−2A-Puro (PX459) V2.0 | Ran et al., 2013 | RRID:Addgene_62988 | |
Transfected construct (Homo sapiens) | MegaTopflash | Hu et al., 2007 | | kind gift from Christophe Fuerer and Roel Nusse, Stanford University |
Transfected construct (Homo sapiens) | CMV Renilla | Promega | E2261 | |
Transfected construct (Homo sapiens) | pSGFP2-C1 | Kremers et al., 2007 | RRID:Addgene_22881 | kind gift from Dorus Gadella |
Transfected construct (Homo sapiens) | pmScarlet-i_C1 | Bindels et al., 2017 | RRID:Addgene_85044 | kind gift from Dorus Gadella |
Transfected construct (Homo sapiens) | pSYFP2-C1 | Kremers et al., 2006 | RRID:Addgene_22878 | kind gift from Dorus Gadella |
Transfected construct (Homo sapiens) | mTurquoise2-C1 | Goedhart et al., 2012 | RRID:Addgene_54842 | kind gift from Dorus Gadella |
Transfected construct (Homo sapiens) | pEGFP | Clontech | | |
Transfected construct (Homo sapiens) | pEGFP2 | Pack et al., 2006 | | kind gift from Masataka Kinjo |
Transfected construct (Homo sapiens) | pEGFP3 | Pack et al., 2006 | | kind gift from Masataka Kinjo |
Transfected construct (Homo sapiens) | pBluescript II KS(+) | Stratagene | | |
Transfected construct (Homo sapiens) | pX459-CTNNB1-ATG | This paper | RRID:Addgene_153429 | |
Transfected construct (Homo sapiens) | pX459-CTNNB1-S45 | This paper | RRID:Addgene_164587 | |
Transfected construct (Homo sapiens) | pRepair-SGFP2-CTNNB1 | This paper | RRID:Addgene_153430 | |
Recombinant DNA reagent | pRepair-mScI-CTNNB1 | This paper | RRID:Addgene_153431 | |
Recombinant DNA reagent | pRepair-SYFP2-CTNNB1 | This paper | RRID:Addgene_153432 | |
Recombinant DNA reagent | pRepair-mTq2-CTNNB1 | This paper | RRID:Addgene_153433 | |
Chemical compound, drug | CHIR99021 | Biovision | 1677–5 | 6 mM stock in DMSO |
Peptide, recombinant protein | Recombinant Mouse Wnt-3a | R and D systems | 1324-WN-002 | 10 µg/ml stock solution in 0.1% BSA in PBS |
Chemical compound, drug | Dapi | Invitrogen | D1306 | |
Chemical compound, drug | Vybrant DyeCycle Violet Stain | Invitrogen | V35003 | |
Chemical compound, drug | Vybrant DyeCycle Ruby Stain | Invitrogen | V10273 | |
Antibody | Non-phosphorylated (Active) β-catenin clone D13A1 (Rabbit monoclonal) | Cell Signaling | 8814S RRID:AB_11127203 | WB (1:1000) |
Antibody | Total β-catenin clone 14 (mouse monoclonal) | BD | 610153 RRID:AB_397554 | WB (1:2000) |
Antibody | α-Tubulin clone DM1A (mouse monoclonal) | Sigma-Aldrich | T9026 RRID:AB_477593 | WB (1:1000) |
Antibody | GFP Antibody (Rabbit polyclonal) | Invitrogen | A-6455 RRID:AB_221570 | WB (1:1000) |
Antibody | IRDye 680LT Goat anti-Rabbit IgG | LI-COR | 926–68021 RRID:AB_10706309 | WB (1:20,000) |
Antibody | IRDye 800CW Donkey anti-Mouse IgG | LI-COR | 926–32212 RRID:AB_621847 | WB (1:20,000) |
Chemical compound, drug | SiR-DNA | Spirochrome | SC007 | |
Chemical compound, drug | Alexa Fluor 488 NHS Ester | Molecular probes | A20000 | |
Software, algorithm | FlowJo | | | |
Software, algorithm | CellProfiler pipeline | This paper | | available at https://osf.io/6pmwf/ |
Software, algorithm | FIJI/ImageJ | | | |
Software, algorithm | FFS Dataprocessor version 2.3 | SSTC | | |
Software, algorithm | ptu converter | Crosby et al., 2013 | | |
Software, algorithm | ImageJ macro script | modified from Crosby et al., 2013 | | available at https://osf.io/ys5qw/ |
Software, algorithm | PlotsOfDifferences | Goedhart, 2019 | | https://huygens.science.uva.nl/PlotsOfDifferences/ |
Software, algorithm | RStudio | | | |
Software, algorithm | R script | This paper | | available at https://osf.io/sxakf/ |
Software, algorithm | R shiny app | This paper | WNT_minimal_model_v2.4.R | app available at https://wntlab.shinyapps.io/WNT_minimal_model/, source script available at https://osf.io/27ya6/ |