Integrative transcriptomic analysis of tissue-specific metabolic crosstalk after myocardial infarction
- KTH Royal Institute of Technology, Sweden
- University of Gothenburg, Sweden
- KTH - Royal Institute of Technology, Sweden
- King's College London, United Kingdom
- Atatürk University, Turkey
- AstraZeneca, Sweden
Abstract
Myocardial infarction (MI) promotes a range of systemic effects, many of which are unknown. Here, we investigated the alterations associated with MI progression in heart and other metabolically active tissues (liver, skeletal muscle, and adipose) in a mouse model of MI (induced by ligating the left ascending coronary artery) and sham-operated mice. We performed a genome-wide transcriptomic analysis on tissue samples obtained 6- and 24-hours post MI or sham operation. By generating tissue-specific biological networks, we observed: (1) dysregulation in multiple biological processes (including immune system, mitochondrial dysfunction, fatty-acid beta-oxidation, and RNA and protein processing) across multiple tissues post MI; and (2) tissue-specific dysregulation in biological processes in liver and heart post MI. Finally, we validated our findings in two independent MI cohorts. Overall, our integrative analysis highlighted both common and specific biological responses to MI across a range of metabolically active tissues.
Data availability
All raw RNA-sequencing data generated from this study can be accessed through accession number GSE153485.
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Revealing tissue-specific metabolic crosstalk after a myocardial infarctionNCBI Gene Expression Omnibus, GSE153485.
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Analysis of early changes in mouse heart transcriptome after myocardial infarctionNCBI Gene Expression Omnibus, GSE104187.
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Long RNA Seq on Sham vs MI Adult Mouse HeartNCBI Gene Expression Omnibus, GSE52313.
Article and author information
Author details
Funding
Knut och Alice Wallenbergs Stiftelse
- Adil Mardinoglu
- Jan Boren
Vetenskapsrådet
- Jan Boren
Hjärt-Lungfonden
- Jan Boren
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: This study were approved by the local animal ethics committee and conform to the guidelines from Directive 2010/63/EU of the European Parliament on the protection of animals used for scientific purposes.
Copyright
© 2021, Arif et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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