The YAP and TAZ paralogs are transcriptional co-activators recruited to target sites by TEAD proteins. Here, we show that YAP and TAZ are also recruited by JUNB (a member of the AP-1 family) and STAT3, key transcription factors that mediate an epigenetic switch linking inflammation to cellular transformation. YAP and TAZ directly interact with JUNB and STAT3 via a WW domain important for transformation, and they stimulate transcriptional activation by AP-1 proteins. JUNB, STAT3, and TEAD co-localize at virtually all YAP/TAZ target sites, yet many target sites only contain individual AP-1, TEAD, or STAT3 motifs. This observation and differences in relative crosslinking efficiencies of JUNB, TEAD, and STAT3 at YAP/TAZ target sites suggest that YAP/TAZ is recruited by different forms of an AP-1/STAT3/TEAD complex depending on the recruiting motif. The different classes of YAP/TAZ target sites are associated with largely non-overlapping genes with distinct functions. A small minority of target sites are YAP- or TAZ-specific, and they are associated with different sequence motifs and gene classes from shared YAP/TAZ target sites. Genes containing either the AP-1 or TEAD class of YAP/TAZ sites are associated with poor survival of breast cancer patients with the triple-negative form of the disease.
All sequencing data were deposited on National Cancer for Biotechnology Information Gene Expression Omnibus (GEO). GSE166943 is the accession number for all the data, with GSE166941 being the subset for the ChIP-seq data and GSE166942 for the RNA-seq data.
YAP and TAZ are co-activators of AP-1 proteins and STAT3 during breast cellular transformationNCBI Gene Expression Omnibus GSE166943.
Regulatory network controlling tumor-promoting inflammation in human cancers (ChIP-seq)NCBI Gene Expression Omnibus GSE115597.
Regulatory network controlling tumor-promoting inflammation in human cancers (RNA-seq)NCBI Gene Expression Omnibus GSE115598.
Genome-scale identification of transcription factors that mediate an inflammatory network during breast cellular transformationNCBI Gene Expression Omnibus GSE100259.
Index and biological spectrum of human DNase I hypersensitive sitesENCODE Project ENCSR752EPH.
- Kevin Struhl
- Zhiping Weng
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Jessica K Tyler, Weill Cornell Medicine, United States
© 2021, He et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.