To investigate the genotype-phenotype landscape, we transgenically expressed wmk homologs with varying degrees of genetic changes. These sequences are codon-optimized based on different codon biases …
(Top) Bayesian nucleotide phylogeny of insect hosts based on 652 bp of the cytochrome oxidase subunit 1 (COI) gene from D. melanogaster, H. bolina, D. bifasciata, C. cautella, D. innubila, D. …
(A) Schematic of wMel, wSuzi, and wRec wmk native nucleotide sequences. The blue tick mark indicates a non-synonymous nucleotide difference. Black tick marks indicate synonymous nucleotide changes. …
Data for sex ratios of closely-related homologs corresponding to Figure 2B.
Statistical output of Kruskal-Wallis test corresponding to sex ratios of closelyrelated homologs in Figure 2B.
Data for qPCR of closely-related transgenes corresponding to Figure 2C.
Statistical output of Kruskal-Wallis test corresponding to qPCR for transgene expression in Figure 2C.
Data for qPCR of msl-2 expression with transgene expression corresponding to Figure 2D.
Statistical output of Kruskal-Wallis test corresponding to qPCR for msl-2 expression in Figure 2D.
(Top) Sex ratios of adult flies are shown for expressing (Act5c-Gal4) and non-expressing (CyO) offspring. Each sample point represents the adult offspring (N = 50–120, mean 84) produced by a …
Data for sex ratios of 5’ alternative start codon transgene expression corresponding to Figure 2—figure supplement 1.
Statistical output of Kruskal-Wallis test corresponding to sex ratios of 5’ alternative start codon transgene expression in Figure 2—figure supplement 1.
Sex ratios of adult flies are shown from either expressing (Act5c-Gal4) or non-expressing (CyO) offspring. WT refers to the background insertion line and Control gene refers to the WD0034 control …
Data for sex ratios of distantly-related homologs in Figure 3.
Statistical output of Kruskal-Wallis test corresponding to sex ratios of divergent homologs in Figure 3.
(A) Sequence alignment of transgenic wmk homologs. The codon farthest on the left is the fourth codon in the sequence, and the highlighted codon is the 16th, with the farthest right representing the …
Data for sex ratios from expression of transgenes with single codon changes corresponding to Figure 4C.
Data for qPCR from expression of transgenes with single codon changes corresponding to Figure 4D.
Data for qPCR from expression of transgenes with single codon changes corresponding to Figure 4D.
Data for qPCR from expression of transgenes with single codon changes corresponding to Figure 4D.
Statistical output of Kruskal-Wallis test corresponding to qPCR from expression of transgenes with single codon changes in Figure 4D.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Wolbachia pipientis) | WD0626 | NCBI | NCBI:WD_RS02815 | Also known as wmk (WO-mediated killing) |
Genetic reagent (D. melanogaster) | Act5c-Gal4/CyO | Bloomington Drosophila Stock Center | BDSC:3953; FlyBase FBti0012290 | P{AyGAL4}25 |
Genetic reagent (D. melanogaster) | WT (y1w67c23; P[CaryP]P2) | Bloomington Drosophila Stock Center | BDSC:8622; FlyBase FBti0040535 | WT strain used in this study; P{CaryP}attP2 |
Genetic reagent (D. melanogaster) | wMel wmk | This paper; Perlmutter et al., 2019; PMID:31504075 | Expresses codon- optimized transgene; UAS promoter | |
Genetic reagent (D. melanogaster) | wBol1b wmk | This paper | Expresses codon- optimized transgene; UAS promoter | |
Genetic reagent (D. melanogaster) | wBif wmk | This paper | Expresses codon- optimized transgene; UAS promoter | |
Genetic reagent (D. melanogaster) | wCaub wmk | This paper | Expresses codon- optimized transgene; UAS promoter | |
Genetic reagent (D. melanogaster) | wInn/wBor wmk | This paper | Expresses codon- optimized transgene; UAS promoter; wInn and wBor wmk have same exact sequence | |
Genetic reagent (D. melanogaster) | wSuzi wmk | This paper | Expresses codon- optimized transgene; UAS promoter | |
Genetic reagent (D. melanogaster) | wRec wmk | This paper | Expresses codon- optimized transgene; UAS promoter | |
Genetic reagent (D. melanogaster) | HA-wmk | This paper | Expresses codon- optimized transgene; UAS promoter; 3 X HA tag epitope in linker between HTH domains of wMel wmk | |
Genetic reagent (D. melanogaster) | 5’ wRec wmk | This paper | Expresses codon-optimized transgene; UAS promoter; Sequence has additional nine amino acids starting at upstream alternative start codon | |
Genetic reagent (D. melanogaster) | 5’ wSuzi wmk | This paper | Expresses codon-optimized transgene; UAS promoter; Sequence has additional nine amino acids starting at upstream alternative start codon | |
Genetic reagent (D. melanogaster) | wMel wmk (new) | This paper | Expresses codon-optimized transgene; UAS promoter; Same exact sequence as wMel wmk, newly transformed strain | |
Genetic reagent (D. melanogaster) | wSuzi codon | This paper | Expresses codon-optimized transgene; UAS promoter; Same as wMel wmk, but with 16th amino acid position using TCC Serine codon from wSuzi wmk strain | |
Genetic reagent (D. melanogaster) | wRec codon | This paper | Expresses codon-optimized transgene; UAS promoter; Same as wMel wmk, but with 16th amino acid position using AGC Serine codon from wRec wmk strain | |
Recombinant DNA reagent | pTIGER (plasmid) | Ferguson et al., 2012; PMID:22328499 | Modified pUASp plasmid for enhanced germline expression under Gal4/UAS control | |
Sequence-based reagent | Rp49_F | This paper | PCR primers | CGGTTACGGAT CGAACAAGC |
Sequence-based reagent | Rp49_R | This paper | PCR primers | CTTGCGCTTCT TGGAGGAGA |
Sequence-based reagent | wmk_homologs_opt_F | This paper | PCR primers | CTGTATGCCATTG CCGAGACCCT |
Sequence-based reagent | wmk_homologs_opt_R | This paper | PCR primers | TCACCAGATCCTTG GCGATCTTCATC |
Sequence-based reagent | Msl-2_F | This paper | PCR primers | GGATTAACGCGGT CTAAGCATGTGTAACTG |
Sequence-based reagent | Msl-2_R | This paper | PCR primers | GTATGCCGTCTG GGCCATGATG |
Commercial assay or kit | Direct-zol RNA MiniPrep Kit | Zymo | R2051 | |
Commercial assay or kit | Superscript VILO cDNA Synthesis Kit | ThermoFisher | 11754050 | |
Chemical compound, drug | DNase, RNase-free | Ambion, Life Technologies | AM2222 | |
Chemical compound, drug | iTaq Universal SYBR Green Mix | Bio-Rad | 1725120 | |
Software, algorithm | GraphPad Prism 8 | GraphPad Prism 8 | RRID:SCR_002798 | |
Software, algorithm | Geneious Pro v.2019.2; Geneious Pro v.2020.2.4 | Geneious | RRID:SCR_010519 | |
Software, algorithm | jModelTest | jModelTest | RRID:SCR_015244 | |
Software, algorithm | RNAfold WebServer | University of Vienna, Gruber et al., 2008, Lorenz et al., 2011 | PMID:18424795; PMID:22115189 | http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi |
**Reagents source from this paper may be obtained from Bordenstein lab.
Source data for all graphical data sets and statistical tests performed for this study.