(A) Illustration of the genomic locus of eif-3.G: Peif-3.G denotes the promoter, blue boxes are exons for coding sequences and gray for 3′UTR. Arrowhead indicates guanine to adenine change in ju807; …
Source data for Figure 1C.
Quantification of convulsions per 60 s in strains of the indicated genotypes.
Source data for Figure 1E.
Quantification of convulsions per 60 s in the indicated strains.
Source data for Figure 1F.
Quantification of convulsions per 60 s in strains. Strain name or genotype is indicated in top row.
(A) Clustal-W sequence alignment of C. elegans EIF-3.G (NP_001263666.1) with the S. cerevisiae (TIF-35; 32% identity; NP_010717.1) and human (eIF3g, 35% identity; AAC78728.1) orthologs. Residues …
(A) Representative confocal z-stack projection images of axonal commissures projecting from motor neurons of wild type or eif-3.G(C130Y) single mutant L4 animals (head to the right). The …
Source data for Figure 1—figure supplement 2A.
Quantification of axonal commissures in strains of the indicated genotypes.
Source data for Figure 1—figure supplement 2B.
Quantification of synaptic puncta in strains of the indicated genotypes.
(A) Quantification of convulsion frequencies in animals of the indicated genotypes; the corresponding strains (left to right) are: MT6241, CZ27434, CZ27435, CZ21759, CZ27433, CZ27436. Error bars are …
Source data for Figure 1—figure supplement 3A.
Quantification of convulsions per 60 s in strains of the indicated genotypes.
Source data for Figure 1—figure supplement 3B.
Quantification of relative fluorescence intensity in the indicated strains.
(A) Quantification of convulsion frequency in animals expressing GFP::EIF-3.G(WT) or GFP::EIF-3.G(C130Y) under Peif-3.G in the indicated genetic backgrounds; and the strains (left to right) are: …
Source data for Figure 2A.
Quantification of convulsions per 60 s in the indicated strains.
Source data for Figure 2B.
Quantification of relative fluorescence intensity in the indicated strains.
Source data for Figure 2C.
Quantification of polysome to monosome ratios in wildtype(N2) and strains of the indicated genotypes.
Top illustration of the EIF-3.G protein showing the EIF-3.I binding region (blue), zinc finger (ZF), RRM (dark grey), Q191* mutation in the EIF-3.G(∆RRM) transgene, RNP motifs (purple), and the RFF …
Source data for Figure 3.
Quantification of convulsions per 60 s in the indicated strains.
(A) Pie charts displaying the proportion of EIF-3.G(WT) and EIF-3.G(C130Y) footprints located within each gene feature. (B) seCLIP read density track of EIF-3.G(WT) and EIF-3.G(C130Y) footprints on …
Source data for Figure 4A.
Number of read clusters representing footprints of EIF-3.G(WT) or EIF-3.G(C130Y) mapping to 5′UTR proximal or 3′UTR regions.
Source data for Figure 4C.
seCLIP reads for EIF-3.G(WT) and EIF-3.G(C130Y) 5’UTR proximal footprints represented as log2(reads per million).
Source data for Figure 4D.
Cumulative EIF-3.G(WT) and EIF-3.G(C130Y) footprint coverage per base distance from the start codon (5’UTR proximal footprints) or stop codon (3’UTR footprints) represented as reads per million.
Source data for Figure 4E.
Length of 5’UTRs in mRNAs expressed in the ACh-MN transcriptome and EIF-3.G targets.
Source data for Figure 4F.
Percent GC of 5’UTRs in mRNAs expressed in the ACh-MN transcriptome and EIF-3.G targets.
(A) Western blotting from strains expressing each indicated 3xFLAG-tagged EIF-3.G transgene in the ACh-MNs under the Punc-17β promoter. Quantification of expression intensity relative to the actin …
Source data for Figure 4—figure supplement 1A.
Quantification of band intensities from western blots using the indicated antibodies in each strain.
Source data for Figure 4—figure supplement 1B.
Number of EIF-3.G(WT), EIF-3.G(C130Y), or EIF-3.G(∆RRM) seCLIP reads mapping to each indicated gene.
(A) Pie charts show the proportion of trans-splicing (ts) among genes with 5′UTR proximal EIF-3.G footprints according to Allen et al. Unannotated genes were absent from the Allen et al. dataset. (B)…
Source data for Figure 4—figure supplement 2A.
Number of EIF-3.G target genes exhibiting trans-splicing.
Source data for Figure 4—figure supplement 2B.
Length of 5’UTRs among trans-spliced or non-trans-spliced mRNAs expressed in the C. elegans transcriptome or EIF-3.G target mRNAs.
Source data for Figure 4—figure supplement 2C.
Percent GC in 10nt bins up to 150nt from the start codon for each indicated gene.
Source data for Figure 4—figure supplement 2D.
Length of 5’UTRs among mRNAs expressed in the human(hg38) transcriptome or human eIF3 target mRNAs.
Source data for Figure 4—figure supplement 2E.
Percent GC in 5’UTRs among mRNAs expressed in the human(hg38) transcriptome or human eIF3 target mRNAs.
(A) Cytoscape network of EIF-3.G target genes with enriched GO terms (neuropeptide signaling, response to stress, and protein translation and protein metabolism) or KEGG pathways (calcium and …
Source data for Figure 5B.
Foldchange in transcript expression in the cholinergic neuronal transcriptome of acr-2(gf) and wild-type animals.
(A) Gene models of hlh-30 isoforms a (pink), b (blue), and d (green), with presumptive promoters for each isoform depicted as right-pointing arrows and the 5′UTR of isoform d in green to the right …
Source data for Figure 6C.
Quantification of relative fluorescence intensity in strains of the indicated genotypes.
Representative single-plane confocal images of hlh-30a isoform-specific reporter (sqIs17, with the expression construct illustrated above) in ACh-MNs in animals of the indicated genotypes. Scale bar …
Source data for Figure 6—figure supplement 1.
Quantification of relative fluorescence intensity in strains of the indicated genotypes.
(A) Illustration of the ncs-2 genomic region. Dark blue represents 5′UTR, green boxes are coding exons, and gray is the 3′UTR. The inset below shows the read density track of seCLIP footprints on …
Source data for Figure 7B.
Quantification of relative fluorescence intensity in strains of the indicated genotypes.
Source data for Figure 7C.
Quantification of relative fluorescence intensity in the indicated strains.
(A) Plot shows conservation of nucleotides (by phyloP135way scores) in the 5′UTR sequences of ncs-2 (blue) and eif-3.G (red), with higher scores indicating greater conservation. Genomic position …
Quantification of relative fluorescence intensity in the indicated strains.
Quantification of relative fluorescence intensity in the indicated strains.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Antibody | anti-FLAG (Rabbit) | Sigma-Aldrich | Cat# F7425, RRID:AB_439687 | WB (1:2000) |
Antibody | anti-Actin clone C4 (Mouse monoclonal) | MP Biomedicals | Cat# 08691002, RRID:AB_2335304 | WB (1:2000) |
Antibody | Anti-FLAG M2 Magnetic Beads | Sigma-Aldrich | Cat# M8823, RRID:AB_2637089 | IP |
Recombinant protein reagent | Cas9-NLS (purified protein) | UC Berkely QB3 | ||
Genetic reagent (C. elegans) | + | CGC | RRID:CGC_N2 | |
Genetic reagent (C. elegans) | acr-2(n2420) X | Jospin et al., 2009 | MT6241 | |
Genetic reagent (C. elegans) | eif-3.G(ju807) II | This work | CZ22197 | Figure 1F |
Genetic reagent (C. elegans) | eif-3.G(ju1840) II | This work | CZ28494 | Figure 1C |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ21759 | Figure 1C |
Genetic reagent (C. elegans) | eif-3.G(ju1840) II; acr-2(n2420) X | This work | CZ28495 | Figure 1C |
Genetic reagent (C. elegans) | eif-3.G(ju1327) / mnC1 II | This work | CZ22974 | Figure 1F |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx7015 | This work | CZ22976 | Figure 1C |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx7016 | This work | CZ22977 | Figure 1C |
Genetic reagent (C. elegans) | eif-3.G(ju807) I;acr-2(n2420) X; juEx7045 | This work | CZ23125 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx7046 | This work | CZ23126 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx7019 | This work | CZ22980 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx7020 | This work | CZ22981 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx7439 | This work | CZ23791 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx7440 | This work | CZ23880 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx7021 | This work | CZ22982 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx7022 | This work | CZ22983 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx8062 | This work | CZ27881 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; juEx8063 | This work | CZ27882 | Figure 1E |
Genetic reagent (C. elegans) | eif-3.G(ju1327) /mnC1 II; acr-2(n2420) X | This work | CZ23310 | Figure 1F |
Genetic reagent (C. elegans) | eif-3.G(ju807) / eif-3.G(ju1327) II | This work | CZ25714 | Figure 1F |
Genetic reagent (C. elegans) | eif-3.G(ju807) / eif-3.G(ju1327) II; acr-2(n2420) X | This work | CZ26828 | Figure 1F |
Genetic reagent (C. elegans) | juSi320 IV | This work | CZ24063 | Figure 2B; Figure 1—figure supplement 1B |
Genetic reagent (C. elegans) | eif-3.G(ju1327) /mnC1 II; juSi320 IV | This work | CZ24079 | Figure 2A |
Genetic reagent (C. elegans) | juSi320 IV; acr-2(n2420) X | This work | CZ24729 | Figure 2A |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; juSi320 IV; acr-2(n2420) X | This work | CZ28107 | Figure 2A |
Genetic reagent (C. elegans) | juSi331 IV | This work | CZ24651 | Figure 2B; Figure 1—figure supplement 1B |
Genetic reagent (C. elegans) | juSi331 IV; acr-2(n2420) X | This work | CZ24652 | Figure 2A |
Genetic reagent (C. elegans) | eif-3.G(ju1327) / mnC1 II; juSi331 IV; acr-2(n2420) X | This work | CZ28497 | Figure 2A |
Genetic reagent (C. elegans) | juIs14 IV | Wang et al., 2017 | CZ631 | |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; juIs14 IV | This work | CZ24161 | Figure 1—figure supplement 2 |
Genetic reagent (C. elegans) | juIs14 IV; acr-2(n2420) X | McCulloch et al., 2020 | CZ5808 | |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; juIs14 IV; acr-2(n2420) X | This work | CZ8905 | Figure 1—figure supplement 2A |
Genetic reagent (C. elegans) | nuIs94 | Hallam et al., 2000 | KP2229 | |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; nuIs94 | This work | CZ24021 | Figure 1—figure supplement 2B |
Genetic reagent (C. elegans) | acr-2(n2420) X; nuIs94 | This work | CZ5815 | Figure 1—figure supplement 2B |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420)X; nuIs94 | This work | CZ24021 | Figure 1—figure supplement 2B |
Genetic reagent (C. elegans) | eif-3.E(ok2607) I / hT2 I,III; acr-2(n2420) X | This work | CZ27434 | Figure 1—figure supplement 3A |
Genetic reagent (C. elegans) | eif-3.E(ok2607) I / hT2 I, III; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ27433 | Figure 1—figure supplement 3A |
Genetic reagent (C. elegans) | eif-3.H(ok1353) I / hT2 I, III; acr-2(n2420) X | This work | CZ27435 | Figure 1—figure supplement 3A |
Genetic reagent (C. elegans) | eif-3.H(ok1353) I / hT2 I, III; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ27436 | Figure 1—figure supplement 3A |
Genetic reagent (C. elegans) | oxSi39 IV | Qi et al., 2013 | CZ12338 | |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; oxSi39 IV | This work | CZ23854 | Figure 1—figure supplement 3B |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx7056 | This work | CZ23203 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx7057 | This work | CZ23204 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8100 | This work | CZ28152 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8101 | This work | CZ28153 | Figure 3 |
Genetic reagent (C. elegans) | juEx7113 | This work | CZ26777 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx7114 | This work | CZ23304 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx7115 | This work | CZ23305 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8095 | This work | CZ28066 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8096 | This work | CZ28067 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8087 | This work | CZ28057 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8088 | This work | CZ28058 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8089 | This work | CZ28064 | Figure 3 |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx8090 | This work | CZ28065 | Figure 3 |
Genetic reagent (C. elegans) | unc-119(tm4063) III; wgIs433 | Sarov et al., 2006 | OP433 | |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; unc-119(tm4063)III; wgIs433 | This work | CZ28145 | Figure 6C |
Genetic reagent (C. elegans) | acr-2(n2420) X; unc-119(tm4063) III; wgIs433 | This work | CZ27913 | Figure 6C |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; unc-119(tm4063) III; acr-2(n2420) X; wgIs433 | This work | CZ27914 | Figure 6C |
Genetic reagent (C. elegans) | sqIs17 | Dittman and Kaplan, 2006 | MAH240 | |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; sqIs17 | This work | CZ28334 | Figure 6—figure supplement 1 |
Genetic reagent (C. elegans) | acr-2(n2420) X; sqIs17 | This work | CZ28212 | Figure 6—figure supplement 1 |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; sqIs17 | This work | CZ28218 | Figure 6—figure supplement 1 |
Genetic reagent (C. elegans) | unc-13(s69) I; wgIs433 | This work | CZ28491 | Figure 6C |
Genetic reagent (C. elegans) | unc-13(s69) I; acr-2(n2420) X; wgIs433 | This work | CZ28492 | Figure 6C |
Genetic reagent (C. elegans) | unc-13(s69) I; eif-3.G(ju807); acr-2(n2420) X; wgIs433 | This work | CZ28493 | Figure 6C |
Genetic reagent (C. elegans) | juSi260 ncs-2(tm1943) I | Zhou et al., 2017 | CZ22459 | |
Genetic reagent (C. elegans) | juSi260 ncs-2(tm1943) I; eif-3.G(ju807) II | This work | CZ23225 | Figure 7B |
Genetic reagent (C. elegans) | juSi260 ncs-2(tm1943) I; acr-2(n2420) X | This work | CZ22345 | Figure 7B |
Genetic reagent (C. elegans) | juSi260 ncs-2(tm1943) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28110 | Figure 7B |
Genetic reagent (C. elegans) | juSi391 ncs-2(tm1943) I | This work | CZ28213 | Figure 7C |
Genetic reagent (C. elegans) | juSi391 ncs-2(tm1943) I; eif-3.G(ju807) II | This work | CZ28340 | Figure 7C |
Genetic reagent (C. elegans) | juSi391 ncs-2(tm1943) I;acr-2(n2420) X | This work | CZ28252 | Figure 7C |
Genetic reagent (C. elegans) | juSi391 ncs-2(tm1943) I;eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28253 | Figure 7C |
Genetic reagent (C. elegans) | juSi392 ncs-2(tm1943) I | This work | CZ28277 | Figure 7—figure supplement 1B |
Genetic reagent (C. elegans) | juSi392 ncs-2(tm1943) I;eif-3.G(ju807) II | This work | CZ28312 | Figure 7—figure supplement 1B |
Genetic reagent (C. elegans) | juSi392 ncs-2(tm1943) I; acr-2(n2420) X | This work | CZ28291 | Figure 7—figure supplement 1B |
Genetic reagent (C. elegans) | juSi392 ncs-2(tm1943) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28292 | Figure 7—figure supplement 1B |
Genetic reagent (C. elegans) | juSi393 ncs-2(tm1943) I | This work | CZ28278 | Figure 7—figure supplement 1C |
Genetic reagent (C. elegans) | juSi393 ncs-2(tm1943) I; eif-3.G(ju807) II | This work | CZ28311 | Figure 7—figure supplement 1C |
Genetic reagent (C. elegans) | juSi393 ncs-2(tm1943) I; acr-2(n2420) X | This work | CZ28293 | Figure 7—figure supplement 1C |
Genetic reagent (C. elegans) | juSi393 ncs-2(tm1943) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ28294 | Figure 7—figure supplement 1C |
Genetic reagent (C. elegans) | juEx2045 | --- | CZ9635 | |
Genetic reagent (C. elegans) | hlh-30(tm1978) IV | CGC | CZ23321 | |
Genetic reagent (C. elegans) | hlh-30(tm1978) IV; acr-2(n2420) X | This work | CZ28174 | Related to Figure 6C |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; hlh-30(tm1978) IV; acr-2(n2420) X | This work | CZ28175 | Related to Figure 6C |
Genetic reagent (C. elegans) | eif-3.G(ju1327) II /mnC1; juSi363 IV; acr-2(n2420) X | This work | CZ26759 | Related to Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | eif-3.G(ju1327) II / mnC1 II; juSi366 IV; acr-2(n2420) X | This work | CZ26760 | Related to Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | juSi364 IV; acr-2(n2420) X | This work | CZ26494 | Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | eif-3.G(ju807)II juSi364 IV; acr-2(n2420) X | This work | CZ26243 | Related to Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | juSi365 IV | This work | CZ26588 | Related to Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; juSi365 IV; acr-2(n2420) X | This work | CZ26565 | Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | juSi365 IV; acr-2(n2420) X | This work | CZ26566 | Related to Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | juSi368 IV | This work | CZ26656 | Related to Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | juSi368 IV; acr-2(n2420) X | This work | CZ26623 | Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; juSi368 IV; acr-2(n2420) X | This work | CZ26480 | Related to Figure 4—figure supplement 1A |
Genetic reagent (C. elegans) | wgIs506 | Sarov et al., 2006 | OP506 | Supplementary file 1 |
Genetic reagent (C. elegans) | acr-2(n2420) X; wgIs506 | This work | CZ27926 | Supplementary file 1 |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; wgIs506 | This work | CZ27927 | Supplementary file 1 |
Genetic reagent (C. elegans) | dhc-1::GFP(it45) I | Lapierre et al., 2013 | OD2955 | Supplementary file 1 |
Genetic reagent (C. elegans) | dhc-1::GFP(it45) I; acr-2(n2420) X | This work | CZ27858 | Supplementary file 1 |
Genetic reagent (C. elegans) | dhc-1::GFP(it45) I; eif-3.G(ju807) II; acr-2(n2420) X | This work | CZ27859 | Supplementary file 1 |
Genetic reagent (C. elegans) | wgIs432 | Sarov et al., 2006 | OP432 | Supplementary file 1 |
Genetic reagent (C. elegans) | acr-2(n2420) X; wgIs432 | This work | CZ27915 | Supplementary file 1 |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; wgIs432 | This work | CZ28021 | Supplementary file 1 |
Genetic reagent (C. elegans) | wgIs638 | Sarov et al., 2006 | OP638 | Supplementary file 1 |
Genetic reagent (C. elegans) | unc-119(tm4063) III;acr-2(n2420) X; wgIs638 | This work | CZ28108 | Supplementary file 1 |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; wgIs638 | This work | CZ27916 | Supplementary file 1 |
Genetic reagent (C. elegans) | let-607(tm1423) I; unc-119(ed3) III; vrIs121 | Sarov et al., 2006 | YL651 | Supplementary file 1 |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; let-607(tm1423) I; unc-119(ed3) III; vrIs121 | This work | CZ28143 | Supplementary file 1 |
Genetic reagent (C. elegans) | acr-2(n2420) X; let-607(tm1423) I;unc-119(ed3) III; vrIs121 | This work | CZ28119 | Supplementary file 1 |
Genetic reagent (C. elegans) | eif-3.G(ju807) II; acr-2(n2420) X; let-607(tm1423) I; unc-119(ed3) III; vrIs121 | This work | CZ28111 | Supplementary file 1 |
Genetic reagent (C. elegans) | juIs172 | CGC | EE86 | |
Genetic reagent (C. elegans) | egl-30(md186) I;dpy-20(e1282ts) IV; syIs105 | CGC | PS4263 | |
Genetic reagent (C. elegans) | juEx7964 | McCulloch et al., 2020 | CZ27420 | |
Genetic reagent (C. elegans) | acr-2(n2420) X; juEx7964 | McCulloch et al., 2020 | CZ27217 | |
Genetic reagent (C. elegans) | eif-3.G(C130Y) II; acr-2(n2420) X; juEx7964 | This work | CZ28109 | Supplementary file 1 |
Recombinant DNA reagent (plasmid) | pCZGY2729 | Andrusiak et al., 2019 | RRID:Addgene_135096 | Site-specific insertion using CRISPR/Cas9 editing of C. elegans ChrIV |
Recombinant DNA reagent (plasmid) | pCZGY2750 | Andrusiak et al., 2019 | RRID:Addgene_135094 | Expresses Cas9 and sgRNA for editing of C. elegans ChrIV |
Recombinant DNA reagent (plasmid) | pCZGY2727 | This work | Site-specific insertion using CRISPR/Cas9 editing of C. elegans ChrI | |
Recombinant DNA reagent (plasmid) | pCZGY2748 | This work | Expresses Cas9 and sgRNA for editing of C. elegans ChrI |
Strains used in this study.
Genotyping primers used in this study.
Constructs and related primers used in this study.
Number of mapped reads in seCLIP replicate datasets obtained after sequencing and CLIPPER filtering.
Number of read clusters detected in each dataset after subtraction of IgG control background.
Number of EIF-3.G footprints detected in each dataset after subtraction of background from both IgG and ∆RRM controls.