(a) Optical setup. The beam paths for epifluorescence/brightfield imaging, ODT, and Brillouin microscopy are shown in light yellow, dark green, and light green, respectively. The laser light …
Scale bar 10 µm.
(a–c) Representative maps of the (a) epifluorescence intensity distribution, (b) longitudinal moduli, and (c) RIs of a HeLa cell. Nuclei are stained with Hoechst (blue), and the nucleolar protein in …
Refractive index, longitudinal modulus, Brillouin shift, and absolute density values of different compartments in HeLa cells.
Representative maps of the (a) Brillouin shift and (b) absolute density of a HeLa cell. Quantitative analysis of (c) the Brillouin shift υB, (d) the absolute density ρ, and (e) peak linewidth ΔB. **p…
(a–c) Representative maps of (a) the epifluorescence intensity distribution, (b) the refractive indices, and (c) the longitudinal moduli of a HeLa cell transfected with a plasmid encoding HttQ103. …
Refractive index, Brillouin shift, and longitudinal modulus values of polyglutamine (polyQ) aggregates and their periphery.
Representative example of (a–c) the fluorescence intensity and (d−f) the RI distribution under control conditions without arsenite, with arsenite, and with arsenite after fixation, respectively. …
Refractive index and longitudinal modulus values of cytoplasm, nucleoplasm, and stress granules (SGs) in P525L HeLa cells after different treatments.
For better comparability, the Brillouin shift υB∗ measured with the 780 nm setup is normalized to a wavelength of 532 nm. Scale bars 10 μm.
FUS-GFP-labeled stress granules induced by oxidative stress show a similar RI as the peripheral cytoplasm. *p<0.05; ****p<0.0001.
(a–d) Representative maps of the (a) epifluorescence intensities, (b) brightfield intensities, (c) RIs, and (d) Brillouin shifts of an adipocyte cell. The nucleus is stained with Hoechst (blue in a) …
Average values and standard errors of the mean of the refractive index (RI) , Brillouin shift , absolute density , longitudinal modulus , and linewidth for the cytoplasm (cyto), nucleoplasm (np), and nucleoli (nl) of 139 wild-type HeLa cells.
Kruskal−Wallis p-values when comparing the refractive index (RI) , Brillouin shifts , mass densities , longitudinal moduli , and linewidths of the cytoplasm (cyto), nucleoplasm (np), and nucleoli (nl) of 139 wild-type HeLa cells, respectively.
Average values and standard errors of the mean of the refractive index (RI) , Brillouin shift , absolute density , and longitudinal modulus for the cytoplasm and polyglutamine (polyQ) aggregates of 22 wild-type HeLa cells.
Average values and standard errors of the mean of the refractive index (RI) and longitudinal modulus for different conditions and compartments of P525L HeLa cells.