(A, B) Lateral view bulk Ca++ activity traces, mean (black), and SD (gray) for (A) animals with suppressed NETHRB (N = 6) and (B) wild-type (WT) pupae (N = 16). Lack of change in activity precludes discrimination between P0 and P1 for NETHRB-suppressed animals. T = 0, imaging onset, all traces aligned to P2 start. (C) Comparison of muscle activity in NETHRB-suppressed (NETHRB) and WT animals during the execution, or attempted execution, of the RollCon movement. Syllables are outlined. Scale bars, 250 μm. (D) Muscle activity montages for NETHRB-suppressed and WT pupae show disruption in posterior muscle activation. PME1 is outlined in WT image. Scale bars, 250 μm. T = 0, movement onset. Times in min:s format. (E) Top: Ca++ activity trace (lateral view) for an animal with suppressed CCAP-expressing neurons (NCCAP). Dotted lines indicate identifiable phase onset times. Black arrow indicates a single partial swing-like movement where P2 typically begins. T = 0, imaging onset. Bottom: mean Ca++ activity (± SD) for 10 NCCAP-suppressed pupae. T = 0, imaging onset, all traces aligned to P1 start. (F) Images of muscle activity during a Swing in WT or a partial swing-like movement in NCCAP-suppressed animals. Schematic on left indicates the A-P boundary (magenta) and ventral, lateral, and dorsal compartments. On the right are Ca++ traces for M3, M13, and M22 in hemisegment (HS) A4 from a single swing for a WT and NCCAP-suppressed animal. Co-incidence across the dorsal (D), lateral (L), and ventral (V) compartments is lost in NCCAP. Scale bars, 250 μm.