(A) In the affected (right) limb, stimulus evoked responses following electrical forepaw stimulation were significantly reduced 1 week after stroke in both groups. Mice recovering spontaneously (–Stim, n=10, top row) exhibited robust activations at 4 weeks, while responses in stimulated mice (+Stim, n=15) were comparable to week 1 responses. (B) Quantification of evoked response magnitude and area in the ipsilesional hemisphere. The 2-way repeated measures ANOVA (rmANOVA) during recovery revealed a significant group × time interaction (F1,23=5.12; p=0.033) for evoked response magnitude and a trending group × time interaction (F1,23=4.0; p=0.057) in response area. Main effects of group (F1,23=9.5; p=0.005) and time (F1,23=4.5; p=0.045) were observed for response area while magnitude also exhibited a main effect of time (F1,23=8.32; p=0.008). (C) In the unaffected (left) limb, –Stim mice exhibited a trend toward increased activity at week 1 that subsided by week 4. In +Stim mice, evoked responses were significantly elevated at weeks 1 and 4 compared to baseline. (D) Quantification of evoked response magnitude and area in the contralesional hemisphere. Over the duration of the experiment, 2-way rmANOVA revealed a significant group x time interaction (F2,46=6.02; p=0.0048), as well as a main effect of time (F1.7,39=9.34; p=0.0009) and group (F1,23=6.2; p=0.02) for evoked response area. A main effect of time (F1.8,41=6.43; p=0.0051) was observed for response magnitude. Post hoc tests were performed as t-tests assuming unequal group variance and corrected for multiple comparisons using false discovery rate correction. All analysis performed using total hemoglobin as contrast. Statistical analyses were performed in Graph Pad Prism 8. *=p<0.05; **=p<0.01; ***=p<0.001; ****=p< 0.0001.