(A) Post-trauma GFP retention in dopaminergic neurons of C. elegans by large-particle flow cytometry with the respective RNAi for ETC subunit components. n=two sorts, n=3344 worms (nuo-2), n=2343 worms (sdhd-1), n=3825 (cyc-1), n=2973 (cox-5b), n=3265 (atp-3), n=1948 (EV). (B) Post-trauma GFP retention in dopaminergic neurons of C. elegans treated with cox-5b/EV RNAi dilutions. n=six sort, n=512 worms (0), n=827 worms (0.25), n=900 worms (0.5), n=832 worms (1). (C) Representative micrographs of tyrosine hydroxylase (TH, green) and cleaved caspase-3 (Casp3, gray) in the midbrain of 10-week-old Surf1+/+ or Surf1-/- mice subject to concussive injury. 1: VTA, 2: SNpc, 3: SNr. Scale bar=200 μm. (D) Quantification of cleaved caspase-3 staining in the midbrain. n=5 Surf1+/+, n=6 Surf1-/-. (E) Latency to fall off rotating rod 7 days after concussive injury (n=four per group). (F) Incidence of laser beam disruptions per mouse over 24 hr (n=three per group). Data are mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, and ****p ≤ 0.0001.