Cell line (Homo sapiens) | MCF7 | American Type Culture Collection | Cat#:HTB-22; RRID:VCL_0031 | |
Cell line (H. sapiens) | T47D | European Collection of Authenticated Cell Cultures | Cat#:85102201; RRID:CVCL_0553 | |
Transfected construct (human) | Edit-R Human HSF1 crRNAs | Dharmacon, Horizon Discovery Group Company | Cat#:CM-012109-02-0002 | Transfected construct (human)5′GGTGTCCGGGTCGCTCACGA |
Transfected construct (human) | Edit-R Human HSF1 crRNAs | Dharmacon, Horizon Discovery Group Company | Cat#:CM-012109-05-0002 | Transfected construct (human)AAAGTGGTCCACATCGAGCA |
Transfected construct (human) | Edit-R Human HSF1 crRNAs | Dharmacon, Horizon Discovery Group Company | Cat#:CM-012109-03-0002 | Transfected construct (human)GTGGTCCACATCGAGCAGGG |
Transfected construct (human) | Edit-R tracrRNA | Dharmacon, Horizon Discovery Group Company | Cat#:U-002005 | Transfected construct |
Antibody | Anti-HSF1 (rabbit polyclonal) | Enzo, Life Sciences, Famingdale, NY | Cat#:ADI-SPA-901; RRID:AB_1083465 | WB (1:4000),IF (1:300)PLA (1:300)ChIP (4 μg/sample) |
Antibody | Anti-HSF1 (E-4) (mouse monoclonal) | Santa Cruz Biotechnology, Inc, Inc, Dallas, TX | Cat#:sc-17757; RRID:AB_627753 | PLA (1:200) |
Antibody | Anti-ERα (estrogen receptor α) (D8H8) (rabbit monoclonal) | Cell Signaling Technology, Danvers, MA | Cat#:8644; RRID:AB_2617128 | WB (1:1000)PLA (1:200) |
Antibody | Anti-ERα (mouse monoclonal) ERalpha | Diagenode, Liège, Belgium | Cat#:C15100066; RRID:AB_2716575 | PLA (1:200)ChIP (4 μg/sample)IF (1:200) |
Antibody | Anti-phosphoERα (S118) (16J4) (mouse monoclonal) | Cell Signaling Technology, Danvers, MA | Cat#:2511; RRID:AB_331289 | WB (1:2000) |
Antibody | Anti-ACTB (AC-15)(HRP) (mouse monoclonal) | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:A3854; RRID:AB_262011 | WB (1:25,000) |
Antibody | Anti-HSP90 (rabbit polyclonal) | Enzo, Life Sciences, USA Famingdale, NY | Cat#:ADI-SPA-836; RRID:AB_10615944 | WB (1:2000)PLA (1:200)IF (1:200) |
Antibody | Anti-HSP70 (HSPA1) (mouse monoclonal) | Enzo, Life Sciences, Famingdale, NY | Cat#:ADI-SPA-810; RRID:AB_10616513 | WB (1:2000) |
Antibody | Anti-HSP105 (rabbit polyclonal) | BioVision, Milpitas, CA | Cat#:3390-100; RRID:AB_2264190 | WB (1:600) |
Antibody | Anti-HSPB8/HSP22 (rabbit polyclonal) | Cell Signaling Technology, Danvers, MA | Cat#:3059; RRID:AB_2248643 | WB (1:1000) |
Antibody | Anti-TDAG51 (PHLDA1) (RN-E62) (mouse monoclonal) | Santa Cruz Biotechnology, Inc, Inc, Dallas, TX | Cat#:sc-23866; RRID:AB_628117 | WB (1:1000) |
Antibody | Anti-EGR3 (A-7) (mouse monoclonal) | Santa Cruz Biotechnology, Inc, Inc, Dallas, TX | Cat#:sc-390967; RRID:AB_2894831 | WB (1:1000) |
Antibody | Anti-HSC70 (HSPA8) (B-6) (mouse monoclonal) | Santa Cruz Biotechnology, Inc, Inc, Dallas, TX | Cat#:sc-7298; RRID:AB_627761 | WB (1:5000) |
Antibody | Anti-mouse IgG (HRP) | Millipore, Billerica, MA | Cat#:AP124P; RRID:AB_90456 | WB (1:5000) |
Antibody | Anti-rabbit IgG (HRP) | Millipore, Billerica, MA | Cat#:AP132P; RRID:AB_90264 | WB (1:2000) |
Antibody | Anti-rabbit IgG (Alexa Fluor 488) | Abcam, Cambridge, Great Britain | Cat#:ab150077; RRID:AB_2630356 | IF (1:200) |
Antibody | Anti-mouse IgG (Alexa Fluor 594) | Abcam, Cambridge, Great Britain | Cat#:ab150116; RRID:AB_2650601 | IF (1:200) |
Recombinant DNA reagent | pLVX-shRNA1 vector | Clontech/Takara Bio USA, Inc. | Cat#:632177 | Lentivirus construct to express a small hairpin RNA (shRNA) |
Recombinant DNA reagent | pLVX-shHSF1 | This paper | | pLVX-shRNA1 vector encoding shRNA specific for HSF1 |
Recombinant DNA reagent | Edit-R hCMV-PuroR-Cas9 Expression Plasmid | Dharmacon, Horizon Discovery Group Company | Cat#:U-005100-120 | Cas9 expression vector |
Sequence-based reagent | qPCR primers | This paper | | See Supplementary files 6–8 |
Sequence-based reagent | shRNA | This paper | | See Materials and methods |
Peptide, recombinant protein | eSpCas9-GFP protein | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:ECAS9GFPPR | |
Commercial assay or kit | Duolink In Situ Red Kit Mouse/Rabbit | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:DUO92101 | |
Commercial assay or kit | ALDEFLUOR Kit | STEMCELL Technologies | Cat#:01700 | |
Commercial assay or kit | The iDeal ChIP-seq Kit for Transcription Factors | Diagenode | Cat#:C01010055 | |
Commercial assay or kit | Direct-Zol RNA MiniPrep Kit | Zymo Research | Cat#:R2052 | |
Commercial assay or kit | μMacs Streptavidin Kit | Miltenyi Biotec, Bergisch Gladbach, Germany | Cat#:130-074-101 | |
Commercial assay or kit | CellTiter 96 AQueous One Solution Assay | Promega; Madison, WI | Cat#:G3580 | |
Commercial assay or kit | SuperSignal West Pico PLUS Chemiluminescent Substrate | Thermo Fisher Scientific, Waltham, MA | Cat#:34577 | |
Commercial assay or kit | QIAseq Ultralow Input Library Kit | Qiagen, Venlo, Netherlands | Catt#:180492 | |
Commercial assay or kit | ECM Cell Adhesion Array kit | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:ECM540 | |
Commercial assay or kit | PCR Master Mix SYBR Green | A&A Biotechnology, Gdynia, Poland | Cat#:2008-100A | |
Chemical compound, drug | 17 beta-estradiol | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:E4389 | |
Chemical compound, drug | 4-Hydroxytamoxifen | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:T176 | |
Chemical compound, drug | Palbociclib, hydrochloride salt | LC Laboratories, Woburn, MA | Cat#:P-7788 | |
Chemical compound, drug | 4-Thiouridine | Cayman Chemical, Ann Arbor, MI | Cat#:16373-100 | |
Chemical compound, drug | Puromycin | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:P8833 | |
Chemical compound, drug | Phalloidin-TRITC | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:P1951 | IF (1:800) |
Software, algorithm | Adobe Photoshop CS6 | Adobe | Version 13.0.1; RRID:SCR_014199 | |
Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | |
Software, algorithm | Samtools | doi:10.1093/bioinformatics/btp352 | RRID:SCR_002105 | |
Software, algorithm | R software | R Foundation for Statistical Computing | Package v.3.6.2; RRID:SCR_001905 | |
Software, algorithm | DESeq2 | doi:10.1158/0008-5472.CAN-13-1070 | RRID:SCR_015687 | |
Software, algorithm | NOISeq | doi:10.1093/nar/gkv711 | Package v.3.12; RRID:SCR_003002 | |
Software, algorithm | FastQC software | https://www.bioinformatics.babraham.ac.uk/projects/fastqc | RRID:SCR_014583 | |
Software, algorithm | Hisat2 | doi:10.1038/nmeth.3317 | Version 2.0.5; RRID:SCR_015530 | |
Software, algorithm | FeatureCounts | doi:10.1093/bioinformatics/btt656 | Version 1.6.5; RRID:SCR_012919 | |
Software, algorithm | ChIPpeakAnno | doi:10.1186/1471-2105-11-237 | Version 3.24.2; RRID:SCR_012828 | |
Software, algorithm | deepTools2 | doi:10.1093/nar/gkw257 | Version 3.5.0; SCR_016366 | |
Software, algorithm | Bowtie2 | doi:10.1038/nmeth.1923 | Version 2.2.9; SCR_016368 | |
Software, algorithm | MEME Suite | doi:10.1093/nar/gkv416 | Version. 5.4.1; RRID:SCR_001783 | |
Software, algorithm | MACS software | doi:10.1038/nprot.2012.101 | Version 1.4.2; RRID:SCR_013291 | |
Software, algorithm | Bedtools software | doi:10.1093/bioinformatics/btq033 | RRID:SCR_006646 | |
Software, algorithm | MedCalc Statistical Software | MedCalc Software Ltd, Ostend, Belgium | Version 19.2.1; RRID:SCR_015044 | |
Software, algorithm | ChIPseeker | Bioconductor package | Version 1.26.2; RRID:SCR_021322 | |
Software, algorithm | TCGAbiolinks package | doi:10.1093/nar/gkv1507 | Version 2.14; RRID:SCR_017683 | |
Software, algorithm | edgeR package | doi:10.1093/bioinformatics/btp616 | Version 3.28.1; RRID:SCR_012802 | |
Software, algorithm | Statistica | TIBCO Software Inc | RRID:SCR_014213 | |
Software, algorithm | MSigDB | doi:10.1073/pnas.0506580102 | RRID:SCR_016863 | |
Other | Deoxyribonuclease I | Worthington Biochemical Corporation | Cat#:LS006333 | |
Other | RNAClean XP beads | Beckman Coulter Life Science, Indianapolis, IN | Cat#:A63987 | |
Other | MTSEA-biotin-XX | Biotium, Fremont, CA | Cat#:90066 | |
Other | DAPI stain | Invitrogen | Cat#:D1306 | 1 µg/ml |
Other | DharmaFECT Duo | Dharmacon, Horizon Discovery Group Company | Cat#:T-2010 | Transfection reagent |
Other | Viromer CRISPR | Lipocalyx GmbH, Halle (Saale), Germany | Cat#:VCr-01LB-01 | Transfection reagent |
Other | cOmplete Protease Inhibitor Cocktail | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:4693116001 | |
Other | PhosSTOP (phosphatase inhibitor tablets) | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:4906837001 | |
Other | Collagen I | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:804592 | |
Other | Collagen IV | Sigma-Aldrich, Merck KGaA, Darmstadt, Germany | Cat#:C55333 | |
Other | Fibronectin | Corning, NY | Cat#:354008 | |