Role of distinct fibroblast lineages and immune cells in dermal repair following UV radiation induced tissue damage
- Queen Mary University of London, United Kingdom
- King's College London, United Kingdom
- German Cancer Research Center (DKFZ), Germany
- The University of Manchester and Salford Royal NHS Foundation Trust, United Kingdom
Abstract
Solar ultraviolet radiation (UVR) is a major source of skin damage, resulting in inflammation, premature ageing and cancer. While several UVR-induced changes, including extracellular matrix reorganisation and epidermal DNA damage, have been documented, the role of different fibroblast lineages and their communication with immune cells has not been explored. We show that acute and chronic UVR exposure led to selective loss of fibroblasts from the upper dermis in human and mouse skin. Lineage tracing and in vivo live imaging revealed that repair following acute UVR is predominantly mediated by papillary fibroblast proliferation and fibroblast reorganisation occurs with minimal migration. In contrast, chronic UVR exposure led to a permanent loss of papillary fibroblasts, with expansion of fibroblast membrane protrusions partially compensating for the reduction in cell number. Although UVR strongly activated Wnt-signalling in skin, stimulation of fibroblast proliferation by epidermal b-catenin stabilisation did not enhance papillary dermis repair. Acute UVR triggered an infiltrate of neutrophils and T cell subpopulations and increased pro-inflammatory prostaglandin signalling in skin. Depletion of CD4 and CD8 positive cells resulted in increased papillary fibroblast depletion, which correlated with an increase in DNA damage, pro-inflammatory prostaglandins and reduction in fibroblast proliferation. Conversely, topical COX-2 inhibition prevented fibroblast depletion and neutrophil infiltration after UVR. We conclude that loss of papillary fibroblasts is primarily induced by a deregulated inflammatory response, with infiltrating T cells supporting fibroblast survival upon UVR-induced environmental stress.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source Data files containing the numerical data used to generate the figures have been provided for all figures.
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Lef1 expression in fibroblasts maintains developmental potential in adult skin to regenerate woundsNCBI Gene Expression Omnibus, GSE153596.
Article and author information
Author details
Funding
Cancer Research UK (C219/A23522)
- Fiona M Watt
Medical Research Council (MR/PO18823/1)
- Fiona M Watt
Wellcome Trust (206439/Z/17/Z)
- Fiona M Watt
Wellcome Trust (WT94028)
- Lesley E Rhodes
NIHR Greater Manchester Patient Safety Translational Research Centre
- Nathan J Hawkshaw
- Lesley E Rhodes
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All animal experiments were subject to local ethical approval and performed under the terms of a UKgovernment Home Office license (PPL 70/8474 or PP0313918).
Human subjects: Ethical approval was granted by the Greater Manchester North NHS research ethics committee (ref:11/NW/0567) for the studies presented in Figure 1 and Figure 6. Details of the time course analysis of UVRchallenged human skin have been reported previously (Hawkshaw NJ et al. 2020). All volunteers provided written informed consent in accordance with the Declaration of Helsinki principles.
Reviewing Editor
- Edward E Morrisey, University of Pennsylvania, United States
Publication history
- Received: June 8, 2021
- Accepted: December 22, 2021
- Accepted Manuscript published: December 23, 2021 (version 1)
- Version of Record published: January 10, 2022 (version 2)
Copyright
© 2021, Rognoni et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Role of distinct fibroblast lineages and immune cells in dermal repair following UV radiation induced tissue damage
eLife 10:e71052.
https://doi.org/10.7554/eLife.71052
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