(A, B) Klf9 expression inferred from LacZ expression intensity in quiescent RGLs, qRGL (GFP+ MCM2 with radial process, arrows), activated RGL, aRGL (GFP+ MCM2+ with radial process, arrowheads) and …
(A) Schematic of wild-type and modified Klf9 alleles. (B) PCR on tail DNA showing expected bands conveying wild-type and conditional alleles. (C) Left: Klf9 in situ hybridization on hippocampal …
(A) Representative low magnification images of Klf9 FISH signal (Klf9 transcripts) in dentate gyrus sections obtained from Gli1CreERT2:Klf9+/+:Ai14 and Gli1CreERT2:Klf9f/f:Ai14 mice following …
(A, B) Two cohorts of adult Sox1tTA: tetO Klf9 mice were used. Klf9 induction in neural stem and progenitors following 3 weeks off Dox significantly reduced the fraction of activated radial-glial …
(A–D) Clonal analysis of sparsely labeled Gli1+ RGLs in adult Gli1CreERT2:Klf9+/+or f/f:Ai14 mice at 7 dpi. (A, C) Representative images of labeled RGL clones and descendants. For example, A top: …
Representative images of labeled radial-glial neural stem cell (RGL) clones and descendants. Identification was based on tdTomato+ morphology and GFAP immunohistochemistry. Z-series of confocal …
(A) Diagram of experimental design for in vivo two-photon imaging experiments. Inset is a high magnification image of a sparsely labeled single RGL in an adult Gli1CreERT2:Klf9+/+:Ai14 mouse. (B) …
(A) Representative two-photon images of RGL cells R1 and R2 in vivo and their respective post hoc fluorescence image. (B) Confocal immunofluorescence images of the same GFAP+/tdTomato+ cells at …
Narrated example of longitudinal imaging of asymmetric neural stem cell (NSC) divisions. Two-photon imaging across days showing two examples of asymmetric division of NSCs (red arrows).
Narrated example of longitudinal imaging of symmetric cell divisions. Two-photon imaging across days showing two examples of symmetric division of neural stem cells (NSCs; blue arrows).
Three-dimensional reconstruction of RGL cells imaged in vivo before undergoing symmetric division. Field of view corresponds to second row of Figure 3B at 18 dpi.
Three-dimensional reconstruction of RGL cells imaged in vivo after undergoing symmetric division. Field of view corresponds to second row of Figure 3B at 30 dpi.
(A) Schematic of experimental workflow to biochemically isolate and sequence translated mRNAs from Gli1+ RGLs (Gli1CreERT2:Rpl22HAf/+:Klf9f/f or +/+ mice). n = 3 mice, 6 dentate gyri/sample, 3 …
Gene ontology annotation (gGOSt, https://biit.cs.ut.ee/gprofiler/gost) of DEGs in Gli1+ RGLs following Klf9 deletion.
Klf9 expression is elevated in quiescent RGLs. Low levels of Klf9 in RGLs are associated with increased activation. Once activated, RGLs lacking Klf9 are biased toward symmetric self-renewal and RGL …
Data are represented as mean ± SEM. * p=0.02, Scale Bar 50 µm.
Complete lists of differentially expressed genes (DEGs) in Gli1+ radial-glial neural stem cells (RGLs) following Kruppel-like factor 9 (Klf9) deletion.
DEGs were defined by at least 1.2-fold change with FDR < 0.05.
Gene ontology annotation (gGOSt, https://biit.cs.ut.ee/gprofiler/gost) of differentially upregulated genes in Gli1+ radial-glial neural stem cells (RGLs) following Kruppel-like factor 9 (Klf9) deletion.
Gene ontology annotation (gGOSt, https://biit.cs.ut.ee/gprofiler/gost) of differentially downregulated genes in Gli1+ radial-glial neural stem cells (RGLs) following Kruppel-like factor 9 (Klf9) deletion.
Statistical Analysis.