In vivo intraoral waterflow quantification reveals hidden mechanisms of suction feeding in fish
- Département Adaptations du Vivant, France
- Université de Paris, INSERM U1284, Center for Research and Interdisciplinarity (CRI), France
- Department of Biology, University of Antwerp, Universiteitsplein 1, Belgium
Figures
Figure 1
Hypothetical waterflow patterns during suction feeding in fish.
(a) Schematic illustration of extraoral flows (blue path line arrows) and hypothetical intraoral flows (red path line arrows) with important anatomical structures involved in food interception (the …
Figure 2 with 3 supplements
Representative sequences of suction feeding for carp (a) and tilapia (b).
Water and food tracer 3D trajectories outside and inside the buccal cavity are represented by a semi-transparent model of a carp (a, left) and a tilapia (b, left) head. The anteroposterior velocity …
-
Figure 2—source data 1
Normalized displacement components along the three cranium-bound orthogonal axes shown in Figure 2 (mean ± SD in %) for the food and water tracers in carp and tilapia.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig2-data1-v2.docx
-
Figure 2—source code 1
R code generating the graphs of Figure 2.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig2-code1-v2.zip
-
Figure 2—source code 2
R code identifying the phases during a suction-feeding sequence used in Figure 2.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig2-code2-v2.zip
Figure 2—figure supplement 1
Histograms of the total distance covered by the food (in black) and the water tracers (in blue) during an entire sequence of suction feeding for each carp (a) and tilapia (b) trial.
-
Figure 2—figure supplement 1—source code 1
R code generating Figure 2—figure supplement 1.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig3-code3-v2.zip
Figure 2—video 1
Video of a suction-feeding sequence of a carp, showing the raw video, the water and food tracer trajectories, and the rigid-body reconstruction.
Figure 2—video 2
Video of a suction-feeding sequence of a tilapia, showing the raw video, the water and food tracer trajectories, and the rigid-body reconstruction.
Figure 3 with 1 supplement
Visualization of the water tracer paths for both the lateral and dorsoventral views during the intake (a), the reverse flow (b), and the backflow (c).
A representative trial is presented on the first row of each phase and a combination of three trials for one individual of carp (carp 02) and three trials for one individual of tilapia (tilapia 01) …
Figure 3—figure supplement 1
Visualization of the water tracer paths for both the lateral and dorsoventral views during the intake (a), the reverse flow (b), and the backflow (c).
A representative trial is presented on the first row of each phase and a combination of three trials for one individual of carp (carp 01) and three trials for one individual of tilapia (tilapia 02) …
Figure 4 with 2 supplements
Boxplots of the mediolateral curvature calculated based on intervals of the anteroposterior trajectory during the intake in carp (a) and tilapia (b).
The lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles), and the white dot gives a 95% confidence interval for comparing medians. The asterisks …
-
Figure 4—source code 1
R code generating the graphs of Figure 4.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig4-code1-v2.zip
Figure 4—figure supplement 1
Visualization of the water tracer trajectories on a dorsoventral view (tx, tz) during the intake for the seven trials of carp 01 and carp 02 (a) and the six trials of tilapia 01 and tilapia 02 (b).
The diagrams represent a lateral view of carp 02 and tilapia 01 with the location of the water tracers at 25% of the intake phase duration with the matching color gradient, implying that tracers …
-
Figure 4—figure supplement 1—source code 1
R code generating the graphs of Figure 4—figure supplement 1.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig5-code5-v2.zip
Figure 4—figure supplement 2
Visualization of the water tracer trajectories on a dorsoventral view (tx, tz) during the intake for the four trials of carp 01 (a), the three trials of carp 02 (b), the three trials of tilapia 01 (c), and the three trials of tilapia 02 (d).
The color gradient corresponds to the initial position of the tracer on the mediolateral axis (tz).
-
Figure 4—figure supplement 2—source code 1
R code generating the graphs of Figure 4—figure supplement 2.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig6-code6-v2.zip
Figure 5 with 1 supplement
Water tracer anteroposterior trajectory, gape, hyoid depression, and opercula abduction during representative suction-feeding sequences in carp (a) and tilapia (b).
The shaded bars in the x-position graphs represent theintake (blue), reverse flow (rf, grey), and backflow (bf, green) phases. The vertical lines in the bone kinematic plots represent the time of …
-
Figure 5—source code 1
R code generating the graphs of Figure 5.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig5-code1-v2.zip
Figure 5—figure supplement 1
Position of the implanted markers and the locators in the carp (a–d) and tilapia (e–h).
The lateral views (a, c, e, g), frontal views (b, f), and dorsal views (d, h). The pharyngeal jaws are transparent, the implanted markers are in black, and the locators added to the rigid bodies are …
Figure 6 with 1 supplement
Results from previous computational fluid dynamics (CFD) studies of suction feeding in fish showing intraoral flow patterns.
Anterior-to-posterior velocities (color scale) and streamlines at the mid-frontal section planes are given, with the respective 3D models of the head in the bottom-left corners. (a) Central jet …
Figure 6—video 1
Video showing the central jet development in a computational fluid dynamics (CFD) model of suction feeding in shiner perch (Cymatogaster aggregata) from Thompson et al., 2018 showing flow patterns at three consecutive instants during suction-feeding event.
Figure 7
Tracer fabrication for X-ray particle tracking and the experimental design.
(a) Construction of the flow tracer particles by piercing 1.4-mm-diameter expanded polystyrene (EPS) spheres on a 0.4-mm-diameter, Vaseline-coated brass rod with a sharpened tip. (b) Flow tracers …
-
Figure 7—source code 1
R code generating the graphs of Figure 7.
- https://cdn.elifesciences.org/articles/73621/elife-73621-fig7-code1-v2.zip
Figure 8 with 1 supplement
Numerical modeling assessment of tracking performance of suction-feeding waterflows.
(a) Cylindrical mouth cavity inside a hemispherical head generating suction trough a transient pressure-outlet-type boundary condition assigned to the back of the cylinder. (b) Path lines of the …
Figure 8—video 1
