Endometrium: Preparing for implantation
The endometrium, the structure that lines the womb, is one of the most fascinating tissues in the human body. Every month, it grows, changes and destroys itself under the influence of the ovarian hormones estrogen and progesterone. In particular, it undergoes a series of modifications, known as decidualization, which include new types of endometrial cells emerging to help the embryo implant and survive until the placenta takes over. This ‘receptive’ phase only lasts a few days and takes place after ovulation, when progesterone levels are high.
How the endometrium can select and support an embryo has been widely researched, especially in the context of assisted reproductive technologies, recurrent miscarriages and implantation failure. Yet, given the ethical considerations of reproductive experiments and the lack of appropriate model systems, scientists still do not have a full grasp on how endometrial decidualization enables implantation.
For decades, the biology of the endometrium has primarily been studied in the laboratory using cells taken from the uterus after surgery. However, due to the way different cell types grow in dishes, certain endometrial cells are better understood than others. For instance, more is known about the stromal cells (which make up the supporting ‘connective’ tissue) than about the epithelial cells which line the endometrium and form glands secreting essential factors.
The recent emergence of organoid cultures that can mimic native tissues in the laboratory may provide a new source of information. Indeed, certain research groups have established ways to grow epithelial cells in three dimensions, while others have created organoids that comprise both epithelial and stromal cells (Wiwatpanit et al., 2020; Cheung et al., 2021; Boretto et al., 2017; Turco et al., 2017). Now, in eLife, Jan Brosens and colleagues – including Thomas Rawlings as first author – report a new laboratory model of the endometrium that can be used to explore the endometrial changes required for embryos to implant (Rawlings et al., 2021).
The team (which is based at the California Institute of Technology, University Hospitals Coventry and Warwickshire NHS Trust, and the Universities of Cambridge and Warwick) generated ‘endometrial assembloids’ that contained both stromal and epithelial cells growing in three dimensions. The cells were able to organize themselves in a manner that resembles the architecture of an actual endometrium, with gland-like structures surrounded by a bed of stromal cells.
A cocktail of ovarian hormones was applied to the assembloids for four days in order to induce decidualization. Single-cell RNA sequencing was then performed to identify different cell populations that had emerged as a response. Surprisingly, after hormone treatment, there were multiple subpopulations of stromal and epithelial cells. All cell populations exhibited unique patterns of gene expression that mapped to a specific phase in the menstrual cycle, including the receptive phase during which the endometrium can welcome an embryo.
Assembloids that had not been exposed to the hormones featured three subtypes of epithelial cells, and two types of stromal cells. However, the assembloids that had undergone decidualization carried three types of epithelial cells and three types of stromal cells: this included, as Rawlings et al. had predicted, a population of epithelial and stromal cells which had become senescent under the influence of the hormone cocktail. In this ‘suspended’ state (which is often associated with aging), cells are unable to divide but they remain biologically active and can release harmful factors that damage neighboring cells. However, senescence in the endometrium may actually be necessary for implantation to take place, a question that Rawlings et al. could explore with their new model.
The team first used a publicly available computational tool to analyze single-cell RNA data: their goal was to assess how the different cell subpopulations interact, and to predict interactions between surface receptors and their ligands. This showed intense communication between epithelial and stromal cell types; more interestingly, subsets of decidual stromal cells communicated with one another resulting in the activation of the tyrosine kinase signaling pathway, which controls many cellular processes. Exposing the assembloids to dasatinib, a cancer drug which inhibits this pathway, eliminated the emergence of decidual stromal cells that were senescent, while increasing the number of non-senescent decidual cells.
This approach allowed Rawlings et al. to model and influence senescence in order to assess its impact on early embryo development. Human embryos cultured in the presence of assembloids not exposed to dasatinib (and therefore containing senescent decidual stromal cells) could increase in diameter and move. However, assembloids treated with dasatinib appeared to restrict embryo growth and movement (Figure 1).
The work by Rawlings et al. highlights the importance of establishing models that closely mimic human physiology, even if it means co-culturing multiple cell types together. In turn, state-of-the-art technologies such as single-cell RNA sequencing – and their associated bioinformatic tools – can deconvolute this complexity one cell at a time. As implantation remains a poorly understood event, these new approaches could finally help to unravel the complex mechanisms that shape the endometrium for pregnancy.
References
-
Scaffold-Free endometrial organoids respond to excess androgens associated with polycystic ovarian syndromeThe Journal of Clinical Endocrinology & Metabolism 105:769–780.https://doi.org/10.1210/clinem/dgz100
Article and author information
Author details
Publication history
- Version of Record published: October 18, 2021 (version 1)
Copyright
© 2021, Kim
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 1,041
- Page views
-
- 78
- Downloads
-
- 2
- Citations
Article citation count generated by polling the highest count across the following sources: PubMed Central, Crossref, Scopus.
Download links
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Further reading
-
- Cell Biology
- Chromosomes and Gene Expression
Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underlying molecular mechanisms remain poorly understood. Here, we investigated the impact of elevated temperature on centromere structure and chromosome segregation during meiosis in Arabidopsis thaliana. Consistent with previous studies, heat stress leads to a decline in fertility and micronuclei formation in pollen mother cells. Our results reveal that elevated temperature causes a decrease in the amount of centromeric histone and the kinetochore protein BMF1 at meiotic centromeres with increasing temperature. Furthermore, we show that heat stress increases the duration of meiotic divisions and prolongs the activity of the spindle assembly checkpoint during meiosis I, indicating an impaired efficiency of the kinetochore attachments to spindle microtubules. Our analysis of mutants with reduced levels of centromeric histone suggests that weakened centromeres sensitize plants to elevated temperature, resulting in meiotic defects and reduced fertility even at moderate temperatures. These results indicate that the structure and functionality of meiotic centromeres in Arabidopsis are highly sensitive to heat stress, and suggest that centromeres and kinetochores may represent a critical bottleneck in plant adaptation to increasing temperatures.
-
- Cell Biology
High-altitude polycythemia (HAPC) affects individuals living at high altitudes, characterized by increased red blood cells (RBCs) production in response to hypoxic conditions. The exact mechanisms behind HAPC are not fully understood. We utilized a mouse model exposed to hypobaric hypoxia (HH), replicating the environmental conditions experienced at 6000 m above sea level, coupled with in vitro analysis of primary splenic macrophages under 1% O2 to investigate these mechanisms. Our findings indicate that HH significantly boosts erythropoiesis, leading to erythrocytosis and splenic changes, including initial contraction to splenomegaly over 14 days. A notable decrease in red pulp macrophages (RPMs) in the spleen, essential for RBCs processing, was observed, correlating with increased iron release and signs of ferroptosis. Prolonged exposure to hypoxia further exacerbated these effects, mirrored in human peripheral blood mononuclear cells. Single-cell sequencing showed a marked reduction in macrophage populations, affecting the spleen’s ability to clear RBCs and contributing to splenomegaly. Our findings suggest splenic ferroptosis contributes to decreased RPMs, affecting erythrophagocytosis and potentially fostering continuous RBCs production in HAPC. These insights could guide the development of targeted therapies for HAPC, emphasizing the importance of splenic macrophages in disease pathology.