Regulation of immune receptor kinase plasma membrane nanoscale organization by a plant peptide hormone and its receptors
- University of Zurich, Switzerland
- Technical University of Munich, Germany
- University of Natural Resources and Life Sciences, Austria
- Fujian Agriculture and Forestry University, China
- University of Freiburg, Germany
Abstract
Spatial partitioning is a propensity of biological systems orchestrating cell activities in space and time. The dynamic regulation of plasma membrane nano-environments has recently emerged as a key fundamental aspect of plant signaling, but the molecular components governing it are still mostly unclear. The receptor kinase FERONIA (FER) controls ligand-induced complex formation of the immune receptor kinase FLAGELLIN SENSING 2 (FLS2) with its co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1), and perception of the endogenous peptide hormone RAPID ALKALANIZATION FACTOR 23 (RALF23) by FER inhibits immunity. Here, we show that FER regulates the plasma membrane nanoscale organization of FLS2 and BAK1. Our study demonstrates that akin to FER, leucine-rich repeat (LRR) extensin proteins (LRXs) contribute to RALF23 responsiveness, regulate BAK1 nanoscale organization and immune signaling. Furthermore, RALF23 perception leads to rapid modulation of FLS2 and BAK1 nanoscale organization, and its inhibitory activity on immune signaling relies on FER kinase activity. Our results suggest that perception of RALF peptides by FER and LRXs actively modulates plasma membrane nanoscale organization to regulate cell surface signaling by other ligand-binding receptor kinases.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Jürgen Kleine-Vehn
Funding
H2020 European Research Council (309858)
- Cyril Zipfel
Deutsche Forschungsgemeinschaft (STE 2448/1)
- Martin Stegmann
H2020 European Research Council (773153)
- Cyril Zipfel
LTF-EMBO (438-2018)
- Julien Gronnier
LTF-EMBO (512-2019)
- Christina M Franck
LTF-EMBO (100-2017)
- Thomas A DeFalco
H2020 European Research Council (639678)
- Jürgen Kleine-Vehn
Swiss National Science Foundation (31003A_182625)
- Cyril Zipfel
Swiss National Science Foundation (31003A_166577/1)
- Christoph Ringli
Austrian Science Fund (P 33044)
- Jürgen Kleine-Vehn
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2022, Gronnier et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Regulation of immune receptor kinase plasma membrane nanoscale organization by a plant peptide hormone and its receptors
eLife 11:e74162.
https://doi.org/10.7554/eLife.74162
