Circadian programming of the ellipsoid body sleep homeostat in Drosophila

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Abstract

Homeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects depend on discrete morning and evening clock neurons, independent of their roles in circadian locomotor activity. In the R5 ellipsoid body sleep homeostat, we identified elevated morning expression of activity dependent and presynaptic gene expression as well as the presynaptic protein BRUCHPILOT consistent with regulation by clock circuits. These neurons also display elevated calcium levels in response to sleep loss in the morning, but not the evening consistent with the observed time-dependent sleep rebound. These studies reveal the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center.

Data availability

Sequencing data have been deposited in GEO under accession code GSE186076

The following data sets were generated

1. Andreani T
2. Rosensweig C
3. Sisobhan S
4. Ogunlana E
5. Kath W
6. Allada R
(2021) Next generation sequencing of isolated R5 ellipsoid body neurons of Drosophila in the morning and evening with and without sleep deprivation
NCBI Gene Expression Omnibus GSE186076.

https://urldefense.com/v3/__https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE186076__;!!Dq0X2DkFhyF93HkjWTBQKhk!G7MMQ_tUj2qWAK3eeotSDFPBYQ2LYYNs9wdThEF-2lZY_O9SgMUEEz7qKdSijUuRDFopK9lCt2o$

Article and author information

Author details

Tomas Andreani

Department of Neurobiology, Northwestern University, Evanston, United States

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-2967-9689
Clark Rosensweig

Department of Neurobiology, Northwestern University, Evanston, United States

Competing interests
The authors declare that no competing interests exist.
Shiju Sisobhan

Department of Neurobiology, Northwestern University, Evanston, United States

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-9715-1029
Emmanuel Ogunlana

Department of Neurobiology, Northwestern University, Evanston, United States

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-7955-4115
William Kath

Department of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, United States

Competing interests
The authors declare that no competing interests exist.
Ravi Allada

Department of Neurobiology, Northwestern University, Chicago, United States

For correspondence
r-allada@northwestern.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-4371-1577

Funding

National Institutes of Health (R01NS106955)

Ravi Allada

Army Research Office (W911NF1610584)

Ravi Allada

National Science Foundation (DMS-1764421)

William Kath
Ravi Allada

Simons Foundation (597491-RWC)

William Kath
Ravi Allada

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.