(A–D) Representative examples of PCDH15 dimers with two bound AuNPs (A, B) or one bound AuNP (C, D) image in stereocilia. On the left-hand side of each panel, a manually annotate model is shown in a view roughly corresponding to the electron beam (top) and from the side (bottom). On the right-hand side are corresponding slices through the electron density. Due to the missing-wedge effect, the resolution of the side-view at the bottom is lower, blurring AuNP and precluding direct visualization of PCDH15. For panels (B) and (D), it is therefore impossible to show AuNPs and PCDH15 in a single projection as the AuNPs are situated above and below PCDH15 in relation to the electron beam. In panels (C) and (D), there are dimeric features in the PCDH15 density, suggesting that these molecules are PCDH15 dimers with one epitope not bound to 39G7. (E) Table detailing numbers and content of collected tomograms. (F) Bar chart quantifying the ratio of PCDH15 molecules labeled by one or two AuNPs. Assuming that AuNPs bind independently to the two epitopes in the PCDH15 dimer results suggests that 86% of all epitopes were labeled and only 2% of PCDH15 molecules were unlabeled.