Modular, robust and extendible multicellular circuit design in yeast

  1. Alberto Carignano
  2. Dai Hua Chen
  3. Cannon Mallory
  4. R Clay Wright
  5. Georg Seelig  Is a corresponding author
  6. Eric Klavins  Is a corresponding author
  1. University of Washington, United States
  2. Virginia Tech, United States

Abstract

Division of labor between cells is ubiquitous in biology but the use of multi-cellular consortia for engineering applications is only beginning to be explored. A significant advantage of multi-cellular circuits is their potential to be modular with respect to composition but this claim has not yet been extensively tested using experiments and quantitative modeling. Here, we construct a library of 24 yeast strains capable of sending, receiving or responding to three molecular signals, characterize them experimentally and build quantitative models of their input-output relationships. We then compose these strains into two- and three-strain cascades as well as a four-strain bistable switch and show that experimentally measured consortia dynamics can be predicted from the models of the constituent parts. To further explore the achievable range of behaviors, we perform a fully automated computational search over all two-, three- and four-strain consortia to identify combinations that realize target behaviors including logic gates, band-pass filters and time pulses. Strain combinations that are predicted to map onto a target behavior are further computationally optimized and then experimentally tested. Experiments closely track computational predictions. The high reliability of these model descriptions further strengthens the feasibility and highlights the potential for distributed computing in synthetic biology.

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Figure 1 - Source Data 1, Figure 2 - Source Data 1, Figure 3 - Source Data 1, Figure 4 - Source Data 1, Figure 5 - Source Data 1 contain the numerical data used to generate the figures

Article and author information

Author details

  1. Alberto Carignano

    Department of Electrical and Computer Engineering, University of Washington, Seattle, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3306-9365
  2. Dai Hua Chen

    Department of Electrical and Computer Engineering, University of Washington, Seattle, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Cannon Mallory

    Department of Electrical and Computer Engineering, University of Washington, Seattle, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. R Clay Wright

    Virginia Tech, Blacksburg, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7125-3943
  5. Georg Seelig

    Department of Electrical and Computer Engineering, University of Washington, Seattle, United States
    For correspondence
    gseelig@uw.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3163-8782
  6. Eric Klavins

    Department of Electrical and Computer Engineering, University of Washington, Seattle, United States
    For correspondence
    klavins@uw.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3805-5117

Funding

Office of Naval Research (N00014-16-1-3189)

  • Alberto Carignano
  • Georg Seelig
  • Eric Klavins

National Science Foundation (1807132)

  • Alberto Carignano
  • Eric Klavins

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Xiaojun Tian

Version history

  1. Received: October 8, 2021
  2. Preprint posted: October 14, 2021 (view preprint)
  3. Accepted: March 20, 2022
  4. Accepted Manuscript published: March 21, 2022 (version 1)
  5. Accepted Manuscript updated: March 22, 2022 (version 2)
  6. Version of Record published: April 11, 2022 (version 3)

Copyright

© 2022, Carignano et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Alberto Carignano
  2. Dai Hua Chen
  3. Cannon Mallory
  4. R Clay Wright
  5. Georg Seelig
  6. Eric Klavins
(2022)
Modular, robust and extendible multicellular circuit design in yeast
eLife 11:e74540.
https://doi.org/10.7554/eLife.74540

Share this article

https://doi.org/10.7554/eLife.74540

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