Strain, strain background (Escherichia coli) | Rosetta 2(DE3)-pLysS | Fisher Scientific | Cat# 714033 | |
Strain, strain background (E. coli) | BL21-DE3-CodonPlus-RIL | Fisher scientific | Cat#50-125-350 | |
Recombinant DNA reagent | Human BicD2-CTD pet28a | GenScript Reference Noell et al., 2019 doi:10.1021/acs.jpclett.9b01865 | Sequence encoding residues 715–804 of human BicD2 cloned into a pet28a vector via the NdeI and XhoI restriction sites | For the protein sequence expressed from this vector, see ‘Supplementary methods’ |
Recombinant DNA reagent | Human Nup358-min pGEX-6P-1 | GenScript Reference Noell et al., 2019doi:10.1021/acs.jpclett.9b01865 | Sequence encoding residues 2148–2240 of human Nup358 cloned into a pGEX-6P-1 vector via the BamHI and XhoI restriction sites | For the protein sequence expressed from this vector, see ‘Supplementary methods’ |
Recombinant DNA reagent | Nup358-min-pGEX-6P-1 | GenScriptThis paper | Modified Nup358-min pGEX-6P-1 vector that includes a SNAP-tag at its C-terminal domain | For the protein sequence expressed from this vector, see ‘Supplementary methods’This vector can be obtained from Dr. Solmaz’s lab |
Recombinant DNA reagent | Nup358-min-zip pGEX-6P-1 | GenScriptThis paper | Modified Nup358-min-pGEX-6P-1 vector that includes a leucine zipper that was added at the C-terminus before the snap-tag | For the protein sequence expressed from this vector, see ‘Supplementary methods’This vector can be obtained from Dr. Solmaz’s lab |
Recombinant DNA reagent | Human Nup358 (residues 2158–2199) pGEX-6P-1 | GenScriptThis paper | Sequence encoding residues 2158–2199 of human Nup358 cloned into a pGEX-6P-1 vector via the BamHI and XhoI restriction sites | Protein overexpression plasmid, which can be obtained from Dr. Solmaz’s lab |
Peptide, recombinant protein | PreScission protease | Cytiva | Cat# 27084301 | |
Peptide, Recombinant protein | Thrombin, human plasma | Fisher Scientific | Cat# 6051951000U | |
Chemical compound, drug | 13C D-Glucose (U-13C6) | Cambridge Isotope Laboratories | Cat# CLM-1396-PK | |
Chemical compound, drug | 15NH4Cl | Cambridge Isotope Laboratories | Cat# NLM-467-10 | |
Chemical compound, drug | cOmplete EDTA-free Protease Inhibitor Cocktail tablets | Roche | Cat# 45-5056489001-EA | |
Software, algorithm | Origin Student 2018 | OriginLab | | CD spectroscopy |
Software, algorithm | Origin GE Microcal ITC200 | OriginLab | | ITC |
Software, algorithm | BioXTAS RAW software suite (version 2.0.3). | Reference Hopkins et al., 2017 | | |
Software, algorithm | ImageJ 1.52v | Reference Schneider et al., 2012 | | |
Software, algorithm | UCSF Chimera (version 1.14) | Resource for Biocomputing, Visualization, and Informatics at the University of California, San FranciscoReference Pettersen et al., 2004 | | |
Chemical compound, drug | Coomassie brilliant blue R-250 | VWR | Cat# VWRV0472-25G | |
Chemical compound, drug | RNase Inhibitor | Promega | N261B | |
Chemical compound, drug | Q-dot 525 streptavidin conjugate | Invitrogen | Q10141MP | |
Chemical compound, drug | Q-dot 565 streptavidin conjugate | Invitrogen | Q10131MP | |
Chemical compound, drug | Q-dot 655 streptavidin conjugate | Invitrogen | Q10121MP | |
Chemical compound, drug | SNAP-Biotin | New England BioLabs | S9110S | |
Chemical compound, drug | Tubulin protein (X-rhodamine): bovine brain | Cytoskeleton, Inc | TL620M-A | |
Chemical compound, drug | Paclitaxel | Cytoskeleton, Inc | TXD01 | |
Recombinant DNA reagent | Bicaudal D homolog 2 isoform 2 (Homo sapiens) | This paper | NCBI:NP_056065.1 | Protein overexpression plasmid, which can be obtained from Dr. Solmaz’s lab |
Biological sample (Bos taurus) | Dynein-dynactin | Bovine brain | | |
Biological sample(B. taurus) | Tubulin | Bovine brain | | |
Software, algorithm | Nikon ECLIPSE Ti microscope | Nikon | | |
Software, algorithm | Nikon NIS Elements | Nikon | | |
Software, algorithm | Andor EMCCD Camera | Andor Technology USA | | |
Software, algorithm | Prism | GraphPad | v7; RRID:SCR_002798 | |
Chemical compound, drug | 2-[Methoxy(polyethyleneoxy)propyl]trimethoxysilane | J&K Scientific | 967192 | |
Chemical compound, drug | n-Butylamine | Acros Organics | A0344582 | |
Software, algorithm | ImageJ Fiji | NIH | 1.53c | |
Software, algorithm | NMRPipe | NIH, reference Delaglio et al., 1995 | | |
Software, algorithm | NMRFAM_SPARKY | Reference Lee et al., 2015 | | |