Splice site prediction was carried out by using SpliceRover. Splice donor sites are given in red, splice acceptor sites in green. SpliceRover calculates splice sites with a score between 0 and 1, but only splice sites with >0.15 were displayed. Sites were displayed in four colors as indicated. Splice sites were numbered with ‘0’, ‘+1’, or ‘+2’, to indicate how the open reading frame is disrupted. Therefore, all splice events between splice sites with identical numbers will be in-frame, while all splice reactions between unequal numbers will result in out-of-frame fusions. Below, the protein domain structure of Spike is displayed to explain, which domains are being deleted by splice events (NTD: N-terminal domain, RBD: (ACE2) receptor-binding domain, RRAR: furin cleavage site, HR1: heptad repeat1, CH: central helix, CD: connector domain, TM: transmembrane domain). White asterisks mark three splice donor sites that are present in the codon-optimized Spike reading frame of the Vaxzevria but not of the Janssen vaccine.