Tadr Is an axonal histidine transporter required for visual neurotransmission in Drosophila
- National Institute of Biological Sciences, Beijing, China
Abstract
Neurotransmitters are generated by de novo synthesis and are essential for sustained, high-frequency synaptic transmission. Histamine, a monoamine neurotransmitter, is synthesized through decarboxylation of histidine by Histidine decarboxylase (Hdc). However, little is known about how histidine is presented to Hdc as a precursor. Here, we identified a specific histidine transporter, TADR (Torn And Diminished Rhabdomeres), which is required for visual transmission in Drosophila. Both TADR and Hdc localized to neuronal terminals, and mutations in tadr reduced levels of histamine, thus disrupting visual synaptic transmission and phototaxis behavior. These results demonstrate that a specific amino acid transporter provides precursors for monoamine neurotransmitters, providing the first genetic evidence that a histidine amino acid transporter plays a critical role in synaptic transmission. These results suggest that TADR-dependent local de novo synthesis of histamine is required for synaptic transmission.
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All data generated or analysed during this study are included in the manuscript and supporting file.
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Author details
Funding
National Natural Science Foundation of China (81870693)
- Tao Wang
National Natural Science Foundation of China (81670891)
- Tao Wang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: The animal work for generating the antisera was conducted following the National Guidelines for Housing and Care of Laboratory Animals in China, and performed in accordance with institutional regulations after approval by the IACUC at NIBS (Reference# NIBS2016R0001).
Copyright
© 2022, Han et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Tadr Is an axonal histidine transporter required for visual neurotransmission in Drosophila
eLife 11:e75821.
https://doi.org/10.7554/eLife.75821
