Neocortical pyramidal neurons with axons emerging from dendrites are frequent in non-primates, but rare in monkey and human

  1. Petra Wahle  Is a corresponding author
  2. Eric Sobierajski
  3. Ina Gasterstädt
  4. Nadja Lehmann
  5. Susanna Weber
  6. Joachim HR Lübke
  7. Maren Engelhardt
  8. Claudia Distler
  9. Gundela Meyer
  1. Ruhr University Bochum, Faculty of Biology and Biotechnology, Developmental Neurobiology, Germany
  2. Heidelberg University, Medical Faculty Mannheim, Mannheim Center for Translational Neuroscience, Institute of Neuroanatomy, Germany
  3. JARA-Institute Brain Structure Function Relationship, Germany
  4. Johannes Kepler University Linz, Faculty of Medicine, Institute of Anatomy and Cell Biology, Austria
  5. Ruhr University Bochum, Faculty of Biology and Biotechnology, Zoology and Neurobiology, Germany
  6. University of La Laguna, Faculty of Medicine, Department of Basic Medical Science, Spain
8 figures, 5 tables and 1 additional file

Figures

Confocal tile scan of dorsal neocortex (premotor area) of P60 infant macaque.

(A) Pyramidal cells were stained with SMI-32/βIV-spectrin to label dendrites and the axon initial segment, respectively. Insets depict neurons with an axon emerging from the soma (B), or from an …

Figure 2 with 5 supplements
Representative axon carrying dendrite (AcD) neurons.

(A1, A2) From rat visual cortex (biocytin, immunofluorescence); (B1, B2) cat visual cortex (immunofluorescence); (C1, C2) ferret visual cortex (biocytin); (D1, D2) macaque premotor cortex (biocytin, …

Figure 2—figure supplement 1
Variations of axon origins of biocytin-stained pyramidal neurons of rat (A–D) and ferret (E–G) visual cortex, and macaque premotor cortex (H–K), and macaque intraparietal sulcus (L).

The neurons in (L), an AcD cell next to a somatic axon cell, reside millimeters from the injection site, they are long projecting layer III pyramidal neurons. Cells with somatic axons are in B, …

Figure 2—figure supplement 2
Variations of axon origins of Golgi-impregnated pyramidal neurons of human temporal cortex, all taken from Individual 2, 56 years of age and sampled from all layers.

Cells with somatic axons are in A, B, C, D, E, F. Cells with shared root configuration are in G, H, I. Cells with axon carrying dendrites are J, K, L, M, N, O. Axons marked by white arrows, axon …

Figure 2—video 1
Neuron classified as AcD cell in macaque cortex.
Figure 2—video 2
Neuron classified as shared root cell in macaque cortex.
Figure 2—video 3
Neuron classified as Von Economo neuron in macaque cortex.
Proportion of axon carrying dendrite (AcD) neurons across species.

(A) Shown are mean ± SEM. of the individual percentages listed in Tables 1–4, which also indicate the staining methods. Numbers above the bars are the total number of pyramidal neurons assessed per …

Figure 3—source data 1

Data and statistical analysis of experiments shown in Figure 3A, B.

https://cdn.elifesciences.org/articles/76101/elife-76101-fig3-data1-v2.xlsx
Within-species areal comparisons.

(A) Upper left is a photomicrograph of one of the coronal sections stained for immunofluorescence. The regions of interest are color coded. Upper right is the macaque brain (after Paxinos et al., …

Proportion of axon carrying dendrite (AcD) cells versus shared root cells.

(A) Data from rat (biocytin), ferret (biocytin), macaque (biocytin, immunofluorescence, Golgi), and human (Golgi). The species cluster along the ordinate as already seen in Figure 3B. The …

Spine density did not systematically differ between regular dendrites (non-axon carrying dendrites [non-AcDs]), dendrites sharing a root with a neighboring axon, and AcDs.

Data from adult rat and ferret biocytin material, values from each cell are connected by a line.

For normalization, the average of the non-AcD has been set to 1, and all values were expressed relative to this. Mann-Whitney rank sum test p-values and the sample size are reported above each plot.

Figure 6—source data 1

Data and statistical analysis of experiments shown in Figure 6.

https://cdn.elifesciences.org/articles/76101/elife-76101-fig6-data1-v2.xlsx
Axon carrying dendrite (AcD) interneurons in human and cat cortex.

(A) Photomicrograph of a representative Golgi-impregnated bitufted neuron with arcade-like initial axon from supragranular layers next to its reconstruction, followed by three further examples of …

Author response image 1

Tables

Table 1
Proportion of pyramidal neurons with AcD: rodents.
Species; cortical area; staining method; age; sexProportion of AcD cells [%];n of cells assessedBy layers: supra %, infra %
Mouse S1 cortex, layer V;Thy1-EGFP/βIV-spectrin immunofluorescence
Adult, female17.42%, 178 cellsn.a.
Adult, female11.78%, 348 cellsn.a.
Adult, female15.79%, 36 cellsn.a.
Adult, female18.84%, 138 cellsn.a.
Adult, male21.93%, 187 cellsn.a.
Adult, male14.82%, 54 cellsn.a.
Adult, male10.17%, 59 cellsn.a.
Average [%], total n of cells15.82%, 1000 cells
Rat visual cortex; biocytin tracing
Adult, male, two hemispheres17.82%, 174 cells15.06%, 17.69%
Adult, male, two hemispheres15.07%, 803 cells15.65%, 16.46%
Average [%], total n of cells16.45%, 977 cells15.36%, 17.08%
  1. AcD, axon carrying dendrite; n.a., not applicable.

Table 2
Proportion of pyramidal neurons with AcD: ungulate, carnivores.
Species; cortical area; staining method; age; sexProportion of AcD cells [%];n of cells assessedBy layers: supra %, infra %
Kitten visual cortex gyral white matter; intracellular Lucifer yellow
P1, P2 (n = 2, sex n.d.)37.93%, 58 cellsn.a.
P10, P11 (n = 2, sex n.d.)44.64%, 56 cellsn.a.
P12, P14 (n = 2, sex n.d.)47.37%, 19 cellsn.a.
Average [%], total n of cells43.31%, 133 cells
Kitten and adult cat visual cortex layer VI; intracellular Lucifer yellow
P1, P5 (n = 2, sex n.d.)20.18%, 114 cellsn.a.
P11 (n = 2, sex n.d.)17.65%, 51 cellsn.a.
P19, P30 (n = 2, sex n.d.)14.89%, 47 cellsn.a.
P52, P60 (n = 2, sex n.d.)20.00%, 30 cellsn.a.
Adult (n = 2, sex n.d.)17.95%, 39 cellsn.a.
Average [%], total n of cells18.13%, 281 cells
Adult cat visual cortex;SMI-32/βIV-spectrin immunofluorescence
Individual 1, 3 months, sex n.d.13.35%, 978 cells14.42%, 14.55%
Individual 2, adult, sex n.d.14.92%, 496 cellsn.d.
Adult cat visual cortex; biocytin tracing
Individual 3, adult, sex n.d.13.44%, 655 cells12.50%, 15.85%
Individual 4, adult, sex n.d.17.14%, 70 cells18.52%, 12.50%
Individual 5, adult, sex n.d.13.93%, 316 cells13.12%, 15.79%
Individual 6, adult, sex n.d.19.13%, 230 cells19.34%,18.37%
Individual 7, adult, sex n.d.12.64%, 174 cells11.49 %, 19.23%
Average [%] individuals 1–7, total n of cells14.94%, 2919 cells14.90%, 16.05%
Ferret visual cortex; biocytin tracing
Individual 1, adult, female16.56%, 302 cells15.95%, 18.57%
Individual 2, adult, female14.66%, 191 cells15.39%, 13.11%
Individual 3, adult, female11.30%, 230 cells10.24%, 14.06%
Individual 4, adult, male13.07%, 329 cells13.45%, 12.26%
Average [%], total n of cells13.90%, 1052 cells13.76%, 14.50%
Pig dorsoparietal cortex;SMI-32/βIV-spectrin immunofluorescence
3 months, European wild boar, female20.11%, 189 cells22.04%, 19.05%
5 months, domestic, sex n.d.20.99%, 181 cellsn.d.
Average [%], total n of cells20.55%, 370 cells22.04%, 19.05%
  1. AcD, axon carrying dendrite; n.a., not applicable; n.d., not determined due to too weak staining.

Table 3
Proportion of pyramidal neurons with AcD: primates - macaque.
Species; cortical area; staining method; age; sexProportion of AcD cells [%];n of cells assessedBy layers: supra %, infra %
Macaca mulatta premotor cortex; biocytin tracing
Individual 1, 11 years, male4.93%, 954 cells2.79%, 11.38%
Individual 2, 7 years, male3.10%, 816 cells2.93%, 6.32%
Individual 3, 5 years, male4.26%, 423 cells3.23%, 6.25%
Macaca mulatta parietal and visual cortex; SMI-32/βIV-spectrin immunofluorescence
Individual 4, 5 years, male5.99%, 717 cells2.59%, 13.95%
Individual 5, 10 years, male6.75%, 681 cells2.51%, 12.72%
Macaca fascicularis parietal cortex;Golgi-Kopsch method
Individual 6, adult, female3.58%, 307 cells4.82%, 6.90%
Individual 7, adult, male3.07%, 228 cells1.57%, 5.67%
Average [%] individuals 1–7, total n of cells4.53%, 4126 cells2.92%, 9.03%
Macaca mulatta visual cortex; biocytin tracing
Individual 8, P60, female4.51%, 377 cells1.28%, 5.88%
Macaca mulatta cingulate cortex;SMI-32/βIV-spectrin immunofluorescence
Individual 8, P60, female5.80%, 500 cells1.28%, 7.85%
Macaca mulatta premotor/M2 cortex;SMI-32/βIV-spectrin immunofluorescence
Individual 8, P60, female5.36%, 1249 cells1.34%, 9.05%
Average [%] individual 8, total n of cells5.22%, 2126 cells1.30%, 7.59%
  1. AcD, axon carrying dendrite.

Table 4
Proportion of pyramidal neurons with AcD: primates - human.
Species; cortical area; staining method; age; sexProportion of AcD cells [%];n of cells assessedBy layers: supra %, infra %
Human temporal lobe;Golgi-Cox method
Individual 1, 53 years, male2.56%, 646 cells1.45%, 4.40%
Individual 2, 56 years, male2.79%, 825 cells0.96%, 4.66%
Human auditory cortex;Golgi-Kopsch method
Individual 3, 63 years, male0.79%, 253 cells0.69%, 0.91%
Individual 4, 71 years, female1.92%, 677 cells0.80%, 2.58%
Individual 5, 75 years, male0.47%, 215 cells0.00%, 0.80%
Individual 6, 88 years, female0.71%, 140 cells0.00%, 1.28%
Individual 7, 56 years, female1.72%, 407 cells1.27%, 2.34%
Human prefrontal agranular cortex;Golgi-Kopsch method
Individual 8, 46 years, female2.02%, 247 cellsn.d.
Individual 9, 77 years, male2.59%, 424 cellsn.d.
Individual 10, 87 years, female1.53%, 653 cellsn.d.
Human visual cortex area 18Golgi-Kopsch method
Individual 10, 87 years, female2.48%, 242 cells1.77%, 3.10%
Average [%] individuals 1–10, total n of cells1.96%, 4729 cells0.99%, 2.87%
Human auditory cortex gyral white matter;Golgi-Kopsch method
Individual 3, 63 years, male8.69%, 115 cells,n.a.
Individual 4, 71 years, female8.88%, 135 cells,n.a.
Individual 5, 75 years, male8.70%, 69 cells,n.a.
Individual 6, 88 years, female9.18%, 98 cells,n.a.
Average [%] individuals 3–6, total n of cells8.86%, 417 cellsn.a.
  1. AcD, axon carrying dendrite.

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Antibodyanti-βIV-spectrin (Rabbit polyclonal)Höfflin et al., 2017self-madeIF (1:500)
Antibodyanti-SMI-32 unphosphorylated neurofilaments (Mouse polyclonal)Covance, Muenster, GermanyCat # SMI-32r, RRID: AB_2315331IF (1:1000)
Antibodyanti-NeuN(Mouse monoclonal)Merck (Millipore), Darmstadt, GermanyCat # MAB377,RRID: AB_2298772IF (1: 1000)
Antibodyanti-Parvalbumin,(Rabbit recombinant)SWANT, Marly SwitzerlandCode No. PV 27IHC (1:5000)
Antibodydonkey anti-rabbit (Alexa-488 polyclonal)Thermo Scientific, Waltham MA, USARRID: AB_2687506IF (1:1000)
Antibodygoat anti-rabbit (biotinylated polyclonal)Dako A/S, Glostrup, DenmarkRRID: AB_2313609IHC (1:1000)
Antibodygoat, anti-mouse(Alexa-568 polyclonal)Invitrogen, Carlsbad, CA, USARRID: AB_2534013IF (1:1000)
Antibodysheep anti-mouse (biotinylated polyclonal)GE Healthcare Life Sciences, Braunschweig Germany,Amersham Cat # RPN1001IHC (1:200)
Chemical compound, drugStreptavidin Alexa-488Thermo Scientific, Waltham MA, USACat #S11223(1:1000)
Chemical compound, drugABC Elite horseradish peroxidaseVector Labs Inc, Burlingame, CA, USA,RRID: AB_2336827(1:250)
OtherDAPI stainInvitrogenD1306(1 µg/mL)
Software, algorithmSigmaStat 12.3Systat Software GmbHFrankfurt am Main, Germany

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