(A) The cartoon schematics for the ‘mechanical-optical’ recording system (left) and the contact model between a spherical probe and the larval fillet (right). Vin was the driving voltage of the … see more
Numerical data for Figure 1.
(A) The mechanical device was mounted on the working stage of a spinning-disk confocal microscope. (B) The mechanical device. Yellow box: the piezo actuator. Cyan box: the beam coupled with a strain … see more
(A) A representative image of c4da. The forces were applied at about 100 μm from the soma, i.e. along the green dashed circle. The representative force application points were marked using the … see more
Numerical data for Figure 2.
(A) Left panel: the representative image showing that c1da made no responses to a 4 mN compressive force. Right panel: Statistical quantification of the responses of c1da to compressive forces (4 … see more
Numerical data for Figure 2—figure supplement 1.
The responses of c4da to distal stimuli versus the pressures measured at 5 μm, 10 μm and 15 μm away from the center of the force (P5 μm, P10 μm, P15 μm). Data were presented as mean ± sem (n=10 … see more
Numerical data for Figure 2—figure supplement 2.
For each probe size, five fibers were used to measure the poking forces. The poking forces had no dependence on the probe size. Mann Whitney U test was used. ns: no significance.
Numerical data for Figure 2—figure supplement 3.
(A) The schematic showing the force application points (filled circles, green: 30 μm probe, red: 60 μm probe, orange: 100 μm probe) on c4da-cti. The dashed concentric circles were 100 and 200 μm in … see more
Numerical data for Figure 3.
(A) The representative images of the mechanosensory molecules in c4da-wt and c4da-cti. c4da-wt (GFP-Piezo): gfp-piezo/ppk-gal4; ppk-cd4-tdtom/+. c4da-cti (GFP-Piezo): gfp-piezo/ppk-gal4; … see more
Numerical data for Figure 3—figure supplement 1.
(A) The responses of c4da (ΔF/F0) to the d-off stimuli (4 mN, 60 μm probe) applied at different positions.The numbers of cells were indicated below the scattered data points. Mann Whitney U test was … see more
Numerical data for Figure 4.
(A) Cartoon schematics for the mechanical model used in the finite element analysis. PP, pressure perpendicular to the cuticle surface. TL, tension parallel with the cuticle surface. (B) … see more
Numerical data for Figure 4—figure supplement 1.
(A) Representative images of c4da-piezoKO (piezoKO; ppk-cd4-tdtom/+), c4da-ppk261 (uas-cd4-tdtom/ppk-gal4; ppk261), c4da-piezoKO, ppkΔ5 (piezoKO, ppkΔ5; ppk-cd4-tdtom/+). Scale bar: 100 μm. (B) … see more
Numerical data for Figure 5.
(A) Representative images and curves showing the responses (ΔF/F0) of the soma (red arrowhead), the homolateral dendrite (position p1: yellow arrowhead) and contralateral dendrite (position p2: … see more
Numerical data for Figure 6.
C4da-wt: ppk-gal4/+; ppk-cd4-tdtom/20×uas-ivs-gcamp6s. Scrambledi: ppk-gal4/20×uas-ivs-gcamp6s, ppk-cd4-tdtom/uas-Scrambledi. Ca-α1Di: ppk-gal4/20×uas-ivs-gcamp6s; ppk-cd4-tdtom/uas-Ca-α1Di. Ca-α1DX1… see more
Numerical data for Figure 6—figure supplement 1.
(A) The expression level of Ca-α1D in wild-type and Ca-α1Di was examined using RT-PCR. Because Ca-α1D expressed in various types of neurons and the number of c4da in the whole animal was small, we … see more
Gel image and numerical data for Figure 6—figure supplement 2.
Left panel: c4da showed a greater sensitivity to localized poking forces and made uniform responses to the forces applied at different dendritic regions (p) proximal stimuli. (d) distal stimuli. … see more
As shown in the picture, the force probe was firmly mounted in the double-layered holder. These glass probes were short and solid, so they would not be bent during the experiments.
The thickness of the cuticle was indicated. The samples were prepared using high pressure freezing and freeze substitution as previously described9.
(A) The representative color map for the distance to the nearest dendrite, in which the distance was labeled by different colors. Scale bar, 100 μm. Random positions were chosen (the red crosses) as … see more
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The asterisk indicated the force application point and the white arrowhead indicated the soma.