(A) Prefrontal cortex injection sites and spread, blue overlays show injections made using previously published coordinates (Rajasethupathy et al., 2015), green overlays show representative viral spreads of each experimental mouse using in-house initial optimisation attempt, n = 3 and n = 3, respectively. Intensity of colours shows number of animals with the corresponding viral spread, (B) Prefrontal cortex injection site and spread of individual injections using in-house optimised coordinates; each overlay shape represents one injection, n = 7. (C) Representative images of dCA1 area of hippocampus, Ci closeup image of CA1, showing no fluorescent signal, (D) ipsilateral claustrum, (E) ACA at +0.8 Bregma, (F) contralateral claustrum, (G) fibres in some thalamic nuclei, (H) fibres striatum, nucleus accumbens lateral septum, and nucleus of the diagonal band, (I) fibres in prefrontal cortex at +1.2 Bregma. A24a, area 24a (infralimbic); A24b, area 24b (cingulate cortex 1); A25, area 25 (dorsal peduncular cortex); A32, area 32 (prelimbic); ac, anterior commissure; ACA, anterior cingulate area; AD, anterodorsal nucleus; AM, anteromedial nucleus; AV, anteroventral nucleus; CA1, Cornu Ammonis 1;; CA3, Cornu Ammonis 3; Cl, claustrum; DG, dentate gyrus; H, habenula; LD, lateral dorsal nucleus; M1, primary motor area; M2, secondary motor area; MD, mediodorsal nucleus; NAs, nucleus accumbens shell area; PVT, paraventricular nucleus of the thalamus; RE, nucleus reuniens; RH, rhomboid nucleus; VPM, ventral posteromedial nucleus. Transduced neurons and their axons are shown in green while DAPI is shown in blue. Scale bars 100 microns.