(A) Cell coating with recombinant gp120 HIV protein. Detection was performed by flow cytometry using the anti-gp120 antibody A32, and a FITC-labeled anti-human secondary antibody. Mean Fluorescence Intensity (MFI) values are shown. (B) Gating strategy used to identify cell killing after the ADCC assay in the different cell subsets. Cell doublets were excluded by forward and side scatter signals and B and myeloid cells discarded based on their high expression of CD20 and CD32. Beads for absolute cell counting were included to calculate ADCC killing by measuring the disappearance of cells in each cell subset. (C) Frequency of expression measured by flow cytometry of CD32 on CD4+ T cells from healthy donors (HD, n=8), Elite controllers (EC, n=7) and ART-treated and virologically-suppressed PLWH (ART, n=15). Median with interquartile range are shown. Statistical comparisons were performed using the Mann-Whitney test. ***p<0.001; ****p<0.0001. (D–E) Correlations of the HIV-reservoir size and CD32 expression. Spearman correlations are shown in samples from HIV-infected individuals. ART-treated and EC are shown in blue and green dots, respectively. (D) Spearman correlation between the total HIV DNA reservoir size and the frequency of expression of CD32 in CD4+ T cells. ART-treated and EC are shown in blue and green dots, respectively. (E) Spearman correlation between HIV RNA levels in CD4+ T cells and the frequency of expression of CD32 in CD4+ T cells. (F–G) Intrinsic susceptibility to NK-mediated ADCC of Naïve (TNA), Stem Cell Memory (TSCM), Central Memory (TCM), Effector Memory (TEM), TCD32dim, and TCD20dim subsets in (F) EC, and (G) HD. Median with interquartile range is shown. Statistical comparisons were performed using the ANOVA Friedman test. *p<0.05; **p<0.01.