1. Cell Biology
  2. Developmental Biology

Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration

  1. Audrey Miller Williams
  2. Seth Donoughe
  3. Edwin Munro
  4. Sally Horne-Badovinac  Is a corresponding author
  1. Department of Molecular Genetics and Cell Biology, University of Chicago, United States
  2. Committee on Development, Regeneration, and Stem Cell Biology, University of Chicago, United States
  3. Institute for Biophysical Dynamics, University of Chicago, United States
https://doi.org/10.7554/eLife.78343
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Cite this article
  1. Audrey Miller Williams
  2. Seth Donoughe
  3. Edwin Munro
  4. Sally Horne-Badovinac
(2022)
Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration
eLife 11:e78343.
https://doi.org/10.7554/eLife.78343
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  3. Download .RIS
8 figures, 3 tables and 1 additional file

Figures

Figure 1
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Introduction to egg chamber rotation and follicle cell protrusions.

(A) Diagram of a stage 6 egg chamber in cross-section. Anterior is left, posterior right. (B) Three-dimensional diagram of an egg chamber with the anterior half shown. Arrows indicate the migration …

Figure 2
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Method used to segment and measure membrane protrusions.

Top row shows an example of a pair of neighboring cells in which one cell is protruding across their shared interface. Bottom row shows a case in which both cells are protruding across the …

Figure 3 with 5 supplements
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Fat2 increases and polarizes follicle cell protrusivity.

(A) Timelapse frames of control, fat2, and CK-666-treated epithelia labeled with a membrane dye. Middle row shows segmented edges. Protrusive edges, defined as edges with average membrane extension …

Figure 3—source data 1

Sample sizes of dataset used to generate plots in Figure 3B-D, Figure 3—figure supplement 1, and Figure 7C.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig3-data1-v2.csv
Figure 3—source data 2

Membrane protrusivity of control, fat2, and CK-666-treated epithelia.

Used to generate Figure 3C.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig3-data2-v2.csv
Figure 3—figure supplement 1
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Membrane extension length and protrusion orientation in individual egg chambers.

(A) Plot showing the ratio of protrusive to total edges, with protrusivity defined in terms of membrane extension longest length (see Methods). In agreement with the average length definition, the …

Figure 3—figure supplement 2
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Actin protrusions are reduced and unpolarized without Fat2 and further reduced without the WAVE complex.

(A) Images showing phalloidin staining of F-actin in control, fat2, and abi-RNAi-expressing epithelia. Bottom row shows the same images with displayed brightness increased. (B) Examples of segmented …

Figure 3—figure supplement 2—source data 1

F-actin enrichment at cell-cell interfaces of control, fat2, and abi-RNAi-expressing epithelia.

Used to generate Figure 3—figure supplement 2C.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig3-figsupp2-data1-v2.csv
Figure 3—figure supplement 2—source data 2

F-actin interface enrichment by angle in control and fat2 epithelia.

Used to generate Figure 3—figure supplement 2.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig3-figsupp2-data2-v2.csv
Figure 3—figure supplement 2—source data 3

F-actin enrichment at leading-trailing interfaces in control and fat2 epithelia.

Used to generate Figure 3—figure supplement 2.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig3-figsupp2-data3-v2.csv
Figure 3—video 1
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Membrane protrusivity of control, fat2, and CK-666-treated epithelia.

Control, fat2, and CK-666-treated epithelia are labeled with a membrane dye. Bottom row shows segmented edges. Protrusive edges, defined as ones with with membrane extensions longer than the 98th …

Figure 3—video 2
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F-actin protrusivity and protrusion polarity of control and fat2 epithelia.

Control, fat2, and abi-RNAi epithelia with F-actin labeled with F-Tractin-tdTomato. The protrusivity of fat2 epithelia is intermediate between that of control and abi-RNAi epithelia. Brightness …

Figure 3—video 3
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Protrusion orientation in control and fat2 epithelia.

Control and fat2 epithelia are labeled with a membrane dye, and arrows indicating the orientation of protrusions are overlayed. Arrows originate at protrusion bases and have lengths proportional to …

Figure 4 with 1 supplement
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Fat2 acts locally across the cell interface to orient membrane protrusions.

(A) Timelapse frame of a fat2 mosaic epithelium with cell membrane labeled, used to evaluate protrusion orientations in control or fat2 cells within a migratory context. Boxes indicate examples of …

Figure 4—video 1
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Membrane protrusion in a fat2 mosaic epithelium.

Used to evaluate protrusion orientations in control or fat2 cells within a migratory context. Boxes indicate examples of leading-trailing interfaces between neighbor pairs with each possible …

Figure 5 with 2 supplements
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Fat2 in each cell concentrates the WAVE complex at the leading edge of the cell behind.

(A) Diagram showing Fat2 localization at the trailing edge and WAVE complex at the leading edge of the basal surface of follicle cells. The WAVE complex subunits referenced in this paper listed. (B) …

Figure 5—source data 1

Sra1 levels at the basal surface of fat2 mosaic epithelia.

Used to generate Figure 5E and F.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig5-data1-v2.csv
Figure 5—figure supplement 1
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Evaluation of endogenous Sra1-GFP functionality.

(A) Images comparing the localization of markers of WAVE complex subunits: Sra1-GFP, Scar antibody, and Abi-mCherry, at the basal surface (top row) and in cross-section (bottom row). (B) Images of …

Figure 5—figure supplement 1—source data 1

Migration speeds of epithelia expressing Sra1-GFP.

Used to generate Figure 5—figure supplement 1D.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig5-figsupp1-data1-v2.csv
Figure 5—figure supplement 2
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Fat2 concentrates the WAVE complex at cell-cell interfaces and polarizes it across the epithelium.

(A) Images of Sra1-GFP at the basal surface in control and fat2 epithelia. (B) Examples of segmented cell-cell interfaces or medial basal surfaces overlaid on Sra1-GFP images. (C) Plot of mean …

Figure 5—figure supplement 2—source data 1

Sra 1 enrichment at cell-cell interfaces of control and fat2 epithelia.

Used to generate Figure 5—figure supplement 2.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig5-figsupp2-data1-v2.csv
Figure 5—figure supplement 2—source data 2

Sra 1 interface enrichment by angle in control and fat2 epithelia.

Used to generate Figure 5—figure supplement 2.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig5-figsupp2-data2-v2.csv
Figure 5—figure supplement 2—source data 3

Sra 1 enrichment at leading-trailing interfaces in control and fat2 epithelia.

Used to generate Figure 5—figure supplement 2.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig5-figsupp2-data3-v2.csv
Figure 5—figure supplement 2—source data 4

Sra 1 levels at the basal surface of cells in fat2 mosaic epithelia.

Used to generate Figure 5—figure supplement 2.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig5-figsupp2-data4-v2.csv
Figure 6 with 4 supplements
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Fat2 stabilizes a region of WAVE complex enrichment in each cell.

(A) Images of phalloidin-stained F-actin and mosaically expressed Sra1-GFP in an entirely fat2 mutant epithelium. Filled and open arrows indicate genotype boundary interfaces with and without …

Figure 6—figure supplement 1
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The WAVE complex is still associated with protrusions in the absence of Fat2.

Timelapse frames showing pairs of cell interfaces from control or fat2 epithelia expressing Sra1-GFP and labeled with a membrane dye. Arrows indicate membrane protrusions. Sra1-GFP is enriched at …

Figure 6—video 1
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Sra1 enrichment at protrusion tips in control and fat2 epithelia.

Pairs of cell edges from control or fat2 epithelia expressing Sra1-GFP and labeled with a membrane dye. Sra1-GFP is enriched at protrusion tips in both control and fat2 epithelia. The control movie …

Figure 6—video 2
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WAVE complex-enriched region dynamics in control and fat2 epithelia.

Part one shows fields of cells from control and fat2 epithelia expressing Sra1-GFP. Part two shows individual cells from the same epithelia, with arrows indicating regions of WAVE complex enrichment …

Figure 6—video 3
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WAVE complex-enriched region dynamics in a fat2 mosaic epithelium.

Used to evaluate WAVE complex-enriched region dynamics in control or fat2 cells within a migratory context. Associated with Figure 6E and F .

Figure 7 with 1 supplement
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Fat2 stabilizes a protrusive region in each cell.

(A) Timelapse frames of control and fat2 epithelia labeled with a membrane dye, showing the position of a cell’s protrusions over time. Top row shows the interfaces and protrusions of one cell and …

Figure 7—source data 1

Frequency of interface protrusion polarity switches in control and fat2 epithelia.

Used to generate Figure 7C.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig7-data1-v2.csv
Figure 7—video 1
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Dynamics of protrusive regions in control and fat2 cells.

Top row shows the interfaces and membrane protrusions of one cell and its neighbors, labeled with a membrane dye. The segmented membrane extensions originating from the centered cell are overlaid in …

Figure 8 with 3 supplements
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Fat2 colocalizes with the WAVE complex across leading-trailing cell-cell interfaces.

(A) Images of cells expressing Abi-mCherry and endogenous full-length Fat2-3xGFP or endogenous Fat2-3xGFP lacking the intracellular domain (Fat2ΔICD), used to assess colocalization. (B) Timelapse …

Figure 8—source data 1

Colocalization of Fat2 and Abi along leading-trailing interfaces.

Used to generate Figure 8E, Figure 8—figure supplement 1.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig8-data1-v2.csv
Figure 8—source data 2

Fat2, Abi, and F-actin distributions along the length of filopodia.

Used to generate Figure 8G.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig8-data2-v2.csv
Figure 8—figure supplement 1
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Ena and Lar are not required for colocalization between Fat2 and the WAVE complex.

(A) Images of cells expressing Fat2-3xGFP and Abi-mCherry in control, lar, and ena-RNAi backgrounds (top 3 rows) or E-cadherin-GFP and Abi-mCherry (bottom row, negative control for colocalization …

Figure 8—figure supplement 1—source data 1

Ena, Abi, and F-actin distributions along the length of filipodia.

Used to generate Figure 8—figure supplement 1.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig8-figsupp1-data1-v2.csv
Figure 8—figure supplement 1—source data 2

Abi enrichment at leading-trailing interfaces in the absence of Lar.

Used to generate Figure 8—figure supplement 1.

https://cdn.elifesciences.org/articles/78343/elife-78343-fig8-figsupp1-data2-v2.csv
Figure 8—video 1
Download asset
Colocalization of puncta of Fat2 and the WAVE complex along leading-trailing interfaces.

The leading-trailing interfaces of two cells expressing Fat2-3xGFP and Abi-mCherry, used to compare the distributions of Fat2 and the WAVE complex over time. The Fat2-3xGFP channel is offset 2 …

Figure 8—video 2
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WAVE complex accumulation at side-facing protrusions away from Fat2.

A side-facing cell-cell interface from an epithelium expressing Abi-mCherry and Fat2-3xGFP. Arrows indicate a site of transient Abi-mCherry accumulation, protrusion, and dissipation with no …

Tables

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Gene (Drosophila melanogaster)AbiNAFLYB:FBgn0020510FlyBase Name: Abelson interacting protein
Gene (Drosophila melanogaster)DlgNAFLYB:FBgn0001624FlyBase Name: discs large 1
Gene (Drosophila melanogaster)E-cadherinNAFLYB:FBgn0003391FlyBase Name: shotgun
Gene (Drosophila melanogaster)EnaNAFLYB:FBgn0000578FlyBase Name: enabled
Gene (Drosophila melanogaster)Fat2 (kug)NAFLYB:FBgn0261574FlyBase Name: kugelei
Gene (Drosophila melanogaster)LarNAFLYB:FBgn0000464FlyBase Name: Leukocyte-antigen- related-like
Gene (Drosophila melanogaster)ScarNAFLYB:FBgn0041781FlyBase Name: SCAR
Gene (Drosophila melanogaster)Sra1 (CYFIP)NAFLYB:FBgn0038320FlyBase Name: Cytoplasmic FMR1 interacting protein
Genetic reagent (Drosophila melanogaster)Abi-mCherry or ubi >Abi-mCherryBloomington Drosophila Stock Center; FLYB:FBrf0227194 (S. Huelsmann)FLYB:FBst0058729; BDSC:58729FlyBase Symbol: P{Ubi-mCherry.Abi}3
Genetic reagent (Drosophila melanogaster)abi-RNAiNational Institute of Genetics, JapanFLYB:FBtp0079430; NIG:9749 R
Genetic reagent (Drosophila melanogaster)E-cadherin-GFPBloomington Drosophila Stock Center; PMID:19429710FLYB:FBst0060584; BDSC:60584FlyBase Genotype: y[1] w*; TI{TI}shg[GFP]
Genetic reagent (Drosophila melanogaster)GFP-Ena or ubi >GFP-EnaBloomington Drosophila Stock Center; FLYB:FBrf0208868 (S. Nowotarski and M. Peiger)FLYB:FBst0028798; BDSC:28798FlyBase Genotype: w*; P{Ubi-GFP.ena}3
Genetic reagent (Drosophila melanogaster)ena-RNAiVienna Drosophila Resource CenterFLYB:FBst0464896; VDRC:43058
Genetic reagent (Drosophila melanogaster)Fat2-3xGFP FRT80BLaboratory of S. Horne-Badovinac; PMID:28292425FLYB:FBal0326664FlyBase Symbol: kug[3xGFP]
Genetic reagent (Drosophila melanogaster)Fat2ΔICD-3xGFP FRT80BLaboratory of S. Horne-Badovinac; PMID:28292425FLYB:FBal0326665FlyBase Symbol: kug[ΔICD.3xGFP]
Genetic reagent (Drosophila melanogaster)fat2 or fat2N103-2 FRT80BLaboratory of Sally Horne-Badovinac; PMID:22413091FLYB:FBal0267777FlyBase Symbol: kug[N103-2]
Genetic reagent (Drosophila melanogaster)UAS >FlpBloomington Drosophila Stock Center; PMID:9584125FFLYB:FBst0004539; BDSC:4539FlyBase Genotype: y[1] w[*]; PUAS-FLP.DJD1
Genetic reagent (Drosophila melanogaster)FRT80BBloomington Drosophila Stock Center; PMID:8404527FLYB:FBti0002073FlyBase Symbol: P{neoFRT}80B
Genetic reagent (Drosophila melanogaster)UAS >F-Tractin-tdTomatoBloomington Drosophila Stock Center; FLYB:FBrf0226873 (T. Tootle); PMID:24995797FLYB:FBst0058989; BDSC:58989FlyBase Genotype: w*; P{UASp-F-Tractin.tdTomato}15 A/SM6b; MKRS/TM2
Genetic reagent (Drosophila melanogaster)ubi >GFP NLS (3 L) FRT80BBloomington Drosophila Stock Center; FLYB:FBrf0108530 (D. Bilder and N. Perrimon)FLYB:FBst0001620; BDSC:1620FlyBase Genotype: w*; P{Ubi-GFP.D}61EF P{neoFRT}80B
Genetic reagent (Drosophila melanogaster)lar13.2 FRT40ABloomington Drosophila Stock Center; PMID:8598047FLYB:FBst0008774; BDSC8774
Genetic reagent (Drosophila melanogaster)larbola1Bloomington Drosophila Stock Center; PMID:11688569FLYB:FBst0091654; BDSC:91654
Genetic reagent (Drosophila melanogaster)MKRS hsFLP/TM6b, CreBloomington Drosophila Stock CenterFLYB:FBst0001501; BDSC:1501y[1] w[67c23]; MKRS, P{hsFLP}86E/TM6B, P{Crew}DH2, Tb[1]
Genetic reagent (Drosophila melanogaster)nanos-Cas9Bloomington Drosophila Stock Center; FLYB:FBrf0223952 (F. Port and S. Bullock); PMID:25002478FLYB:FBst0054591; BSDC:54591FlyBase Genotype: y[1] M{nos-Cas9.P}ZH-2A w*
Genetic reagent (Drosophila melanogaster)ubi >mRFP NLS (3 L) FRT80BBloomington Drosophila Stock Center; FLYB:FBrf0210705 (J. Lipsick)FLYB:FBti0129786; BDSC:30852FlyBase Genotype: w1118; P{Ubi-mRFP.nls}3 L P{neoFRT}80B
Genetic reagent (Drosophila melanogaster)FRT82b ubi >mRFP NLS (3 R)Bloomington Drosophila Stock Center; FLYB:FBrf0210705 (J. Lipsick)FLYB:FBst0030555; BDSC:30555FlyBase Genotype: w1118; P{neoFRT}82B P{Ubi-mRFP.nls}3 R
Genetic reagent (Drosophila melanogaster)Sra1-GFPProduced for this studySra1 endogenously tagged with GFP using CRISPR. Available from Horne-Badovinac Lab upon request to shorne@uchicago.edu
Genetic reagent (Drosophila melanogaster)Sra1-GFP FRT80BProduced for this studySra1 endogenously tagged with GFP using CRISPR, with FRT80B. Available from Horne-Badovinac Lab upon request to shorne@uchicago.edu
Genetic reagent (Drosophila melanogaster)sra1-RNAiBloomington Drosophila Stock Center; PMID:21460824FLYB:FBst0038294; BDSC:38294FlyBase Genotype: y[1] sc* v[1] sev[21]; P{TRiP.HMS01754}attP2
Genetic reagent (Drosophila melanogaster)tj >Gal4National Institute of Genetics, Japan; PMID:12324948FLYB:FBtp0089190; DGRC:104055FlyBase Symbol: P{tj-GAL4.U}
Genetic reagent (Drosophila melanogaster)w1118Bloomington Drosophila Stock CenterFLYB:FBal0018186
AntibodyDiscs Large; Dlg (mouse monoclonal)Developmental Studies Hybridoma BankDSHB:4F3; RRID:AB_528203(1:20)
AntibodyScar (mouse monoclonal)Developmental Studies Hybridoma BankAB_2618386(1:200)
AntibodyAlexa Fluor 647, anti-mouse secondary (donkey polyclonal)Thermo Fisher ScientificCat:A31571; RRID:AB_162542(1:200)
Chemical compound, drugCellMask Orange Plasma Membrane StainThermo Fisher ScientificCat:C1004515 min (1:250)
Chemical compound, drugCellMask Deep Red Plasma Membrane StainThermo Fisher ScientificCat:C1004615 min (1:250)
Chemical compound, drugTRITC PhalloidinMillipore SigmaCat:195115 min at room temp (1:300)
Chemical compound, drugAlexa Fluor 647 phalloidinThermo Fisher ScientificCat:C100452 hr at room temp (1:50)
Chemical compound, drugCK-666, Arp2/3 complex inhibitorMillipore SigmaCat:553502750 μM
Chemical compound, drugFormaldehyde, 16%, methanol free, ultra purePolysciencesCat:18814–10
Chemical compound, drugRecombinant human insulinMillipore SigmaCat:12643
Recombinant DNA reagentplasmid: pU6-BbsI-chiRNAAddgeneAddgene:45946; RRID:Addgene_45946PMID:23709638
Recombinant DNA reagentplasmid: pU6 chiRNA Sra1 C-termProduced for this studyCRISPR chiRNA construct for generation of Sra1-GFP. available from Horne-Badovinac Lab upon request to shorne@uchicago.edu
Recombinant DNA reagentplasmid: pDsRed-attPAddgeneAddgene:51019; RRID:Addgene_51019PMID:24478335. Vector used to make pDsRed-attP Sra1-GFP HR
Recombinant DNA reagentplasmid: pTWGDrosophila Genome Resource CenterDGRC:1076source of enhanced GFP for generation of Sra1-GFP
Recombinant DNA reagentplasmid: pDsRed-attP Sra1-GFP HRProduced for this studyCRISPR homologous recombinaton construct for generation of Sra1-GFP. Available from Horne-Badovinac Lab upon request to shorne@uchicago.edu
Software, algorithmZen BlueZeiss
Software, algorithmMetaMorphMolecular Devices
Software, algorithmFIJI (ImageJ)PMID:22743772
Software, algorithmGraphPad Prism 9 for MacGraphPad Software
Software, algorithmMicrosoft Excel for Mac, version 16.47Microsoft
Software, algorithmPython 3Python Software Foundation
Software, algorithmimageioimageio contributors
Software, algorithmmatplotlibThe Matplotlib Development team
Software, algorithmnaparinapari contributors
Software, algorithmnumpynumpy contributors
Software, algorithmpimspims contributors
Software, algorithmpandaspandas contributors
Software, algorithmscikit-imagescikit-image development team
Software, algorithmscikit-ffmscikit-fmm contributors
Software, algorithmscipyscipy contributors
Table 1
Experimental genotypes.
FigurePanelNameGenotype
1DF-actin +Ena + Abiw; ubi >GFP-EnaBDSC:28798/ubi >Abi-mCherryBDSC:58729
1FControlw; tj >Gal4DGRC:104055/+
1Fena-RNAiw; tj >Gal4DGRC:104055/UAS-ena-RNAiVDRC:43058
1Fabi-RNAiw; tj >Gal4DGRC:104055/+; UAS-abi-RNAiNIG:9749R-3
2Top rowProtrusion in 1 directionw1118
2Bottom rowProtrusion in both directionsw; fat2N103-2 FRT80B
3A-CControlw1118
3A-Cfat2w; fat2N103-2 FRT80B
3A-CCK-666w1118
3DControlw1118
3Dfat2w; fat2N103-2 FRT80B
4A,Bfat2 mosaicw; tj >Gal4DGRC:104055, UAS >FlpBDSC:4539/+; fat2N103-2 FRT80B/ubi >GFP NLS FRT80BBDSC1620
5BSra1-GFP mosaicw; tj >Gal4DGRC:104055, UAS >FlpBDSC:4539/+; FRT82B Sra1-GFP/FRT82B ubi >mRFP-NLSBDSC:30555
5Cfat2 mosaicw; tj >Gal4DGRC:104055, UAS >FlpBDSC:4539/+; fat2N103-2 FRT80B/ubi >GFP NLS FRT80BBDSC1620
5D-FSra1-GFP mosaicw; tj >Gal4DGRC:104055, UAS >FlpBDSC:4539/+; fat2N103-2 FRT80B Sra1-GFP/ubi >mRFP NLS FRT80BDSC:30852
6ASra1-GFP mosaic +fat2w; tj >Gal4DGRC:104055, UAS >FlpBDSC:4539/+; fat2N103-2 FRT80B Sra1-GFP/fat2N103-2 FRT80B FRT82B
6B,DControlw;; Sra1-GFP/+
6B,Dfat2w;; fat2N103-2 FRT80B Sra1-GFP/fat2N103-2 FRT80B
6E,Ffat2 mosaic +Sra1w; tj >Gal4DGRC:104055, UAS >FlpBDSC:4539/+; fat2N103-2 FRT80B Sra1-GFP/ubi >mRFP NLS FRT80BDSC:30852
7A,CControlw1118
7A,Cfat2w;; fat2N103-2 FRT80B
7BExample of switchw;; fat2N103-2 FRT80B
8A,EFat2 +Abiw;; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
8A,EFat2ΔICD + Abiw;; ubi >Abi-mCherryBDSC:58729, Fat2ΔICD-3xGFP FRT80B/Fat2ΔICD-3xGFP FRT80B
8BFat2 +Abiw;; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
8C,D,F,GFat2 +Abi + F-actinw;; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
3S1AControlw1118
3S1Afat2w;; fat2N103-2 FRT80B
3S1ACK-666w1118
3S1BControlw1118
3S1Bfat2w;; fat2N103-2 FRT80B
3S2A-CControlw; tj >Gal4DGRC:104055/+
3S2A-Cfat2w; tj >Gal4DGRC:104055/+; fat2N103-2 FRT80B
3S2A-Cabi-RNAiw; tj >Gal4DGRC:104055/+UAS-abi-RNAiNIG:9749R-3/+
3S2DControlw; tj >Gal4DGRC:104055/UAS >F-Tractin-tdTomatoBDSC:58989
3S2Dfat2w; tj >Gal4DGRC:104055/UAS >F-Tractin-tdTomatoBDSC:58989; fat2N103-2 FRT80B
3S2Dabi-RNAiw; tj >Gal4DGRC:104055/UAS >F-Tractin-tdTomatoBDSC:58989; UAS-abi-RNAiNIG:9749R-3/+
3S2E,FControlw1118
3S2E,Ffat2w;; fat2N103-2 FRT80B
5S1ASra1-GFPw;; Sra1-GFP
5S1Aanti-SCARw;; Sra1-GFP
5S1Aubi >Abi-mCherryw;; ubi >Abi-mCherryBDSC:58729/+
5S1BControlw; tj >Gal4DGRC:104055/+
5S1Babi-RNAitj >Gal4DGRC:104055/+; UAS-abi-RNAiNIG:9749R-3/+
5S1CControlw1118
5S1CSra1-GFP x1w;; Sra1-GFP/+
5S1CSra1-GFP x2w;; Sra1-GFP
5S1Csra1-RNAiw; tj >Gal4DGRC:104055/+; UAS >sra1-RNAiBDSC:38294/+
5S1DControlw1118
5S1DSra1-GFP x1w;; Sra1-GFP/+
5S1DSra1-GFP x2w;; Sra1-GFP
5S2A, C-EControlw;; Sra1-GFP/+
5S2A, C-Efat2w;; fat2N103-2 FRT80B Sra1-GFP/fat2N103-2 FRT80B
6S1Controlw;; Sra1-GFP/+
6S1fat2w;; fat2N103-2 FRT80B Sra1-GFP/fat2N103-2 FRT80B
8S1A,BControl Fat2 +Abiw;; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
8S1A,BFat2ΔICD + Abiw;; ubi >Abi-mCherryBDSC:58729, Fat2ΔICD-3xGFP FRT80B/Fat2ΔICD-3xGFP FRT80B
8S1A,Bena-RNAi, Fat2 +Abiw; tj >Gal4DGRC:104055/UAS >ena RNAiVDRC:43058; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
8S1A,BControl Fat2 +Abiw; larbola 1BDSC:91654/lar13.2 BDSC:8774 FRT40A; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
8S1CFat2 +Abiw;; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
8S1D-FEna +Abi + F-actinw; ubi >GFP-EnaBDSC:28798/ubi >Abi-mCherryBDSC:58729
8S1GControlw;; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
8S1Glarw; larbola 1BDSC:91654/lar13.2 BDSC8774 FRT40A; ubi >Abi-mCherryBDSC:58729, Fat2-3xGFP FRT80B/Fat2-3xGFP FRT80B
Table 2
Yeasting conditions.
FigurePanelDays on yeastTemp. (°C)
1D2–325
1F329
22–325
3A-D2–325
4A,B2–325
5B725
5C325
5D-F325
6A525
6B,D2–325
6E2–325
7A-C2–325
8A,E2–325
8B2–325
8C,D,F,G2–325
S1A-C2–325
S2A-C,E,F2–329
S2D2–329
S3A2–325
S3B329
S3C329
S3D2–325
S4A-E2–325
S52–325
S6A,B,F329
S6C2–325
S6D-F2–325

Additional files

Transparent reporting form
https://cdn.elifesciences.org/articles/78343/elife-78343-transrepform1-v2.pdf

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