Higher-order unimodal olfactory sensory preconditioning in Drosophila
- Department of Biology, Georgetown University, United States
- Department of Biological Sciences and Neuroscience & Mental Health Institute, University of Alberta, Canada
- Interdisciplinary Program in Neuroscience, Georgetown University, United States
Figures
Figure 1 with 1 supplement
One-second interstimulus interval (ISI) during preconditioning induces depression to non-trained, pre-paired odor S2 in control flies.
(A) Diagram of the in vivo, under the microscope training imaging setup, MBON-γ1pedc>α/β diagram, and Rac1N17 expression induction. (B) Diagram of the experimental setup (odor schedule): …
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Figure 1—source data 1
One-second interstimulus interval (ISI) during preconditioning induces depression to non-trained, pre-paired odor S2 in control flies.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
Rac1 inhibition induces depression to non-trained, pre-paired odor S2 in MBON-γ1pedc>α/β.
(A) Diagram of experimental setup (odor schedule): preconditioning responses were obtained for 4-methylcyclohexanol (MCH) and 3-octanol (OCT) (S1 and S2); later flies were aversively trained to MCH …
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Figure 1—figure supplement 1—source data 1
Rac1 inhibition induces depression to non-trained, pre-paired odor S2 in MBON-γ1pedc>α/β.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig1-figsupp1-data1-v2.xlsx
Figure 2 with 1 supplement
Rac1 inhibition induces depression to sensory preconditioned odor S2 in MBON-γ1pedc>α/β using a different odor pair with orthogonal representation.
(A) Diagram of experimental setup (odor schedule): preconditioning responses were obtained for penthyl acetate (PA) and ethyl lactate (EL) (S1 and S2, respectively); later flies were aversively …
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Figure 2—source data 1
Rac1 inhibition induces depression to sensory preconditioned odor S2 in MBON-γ1pedc>α/β using a different odor pair with orthogonal representation.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig2-data1-v2.xlsx
Figure 2—figure supplement 1
Rac1 inhibition does not induce depression to repeated odor presentation in MBON-γ1pedc>α/β when the electric shock is excluded.
(A) Upper part, diagram of experimental setup (odor schedule): preconditioning responses were obtained for 4-methylcyclohexanol (MCH) and 3-octanol (OCT) (S1 and S2); later flies were presented with …
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Figure 2—figure supplement 1—source data 1
Rac1 inhibition does not induce depression to repeated odor presentation in MBON-γ1pedc>α/β when the electric shock is excluded.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig2-figsupp1-data1-v2.xlsx
Figure 3
Excluding preconditioning eliminates depression to the S2 odor.
(A) Diagram of experimental procedure (odor schedule): Flies were aversively trained to 4-methylcyclohexanol (MCH) (S1) and 5 min later post-conditioning responses to four odors were recorded …
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Figure 3—source data 1
Excluding preconditioning eliminates depression to the S2 odor.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig3-data1-v2.xlsx
Figure 4 with 4 supplements
Repeated presentations of paired odors (S1/S2) induce behavioral sensory preconditioning.
(A) Flies were trained using an Arduino microcontroller for precise odor delivery: Flies were preconditioned by 10 repeated presentations of S1/S2 odor pairs with 1-s interstimulus interval (ISI). …
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Figure 4—source data 1
Repeated presentations of paired odors (S1/S2) induce behavioral sensory preconditioning.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig4-data1-v2.xlsx
Figure 4—figure supplement 1
A single presentation of paired odors (S1/S2) does not induce behavioral sensory preconditioning.
(A) Control flies were trained using an Arduino microcontroller for precise odor delivery. Diagram of experimental setup (odor schedule): Flies were preconditioned by a single presentation of a …
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Figure 4—figure supplement 1—source data 1
A single presentation of paired odors (S1/S2) does not induce behavioral sensory preconditioning.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig4-figsupp1-data1-v2.xlsx
Figure 4—figure supplement 2
Repeated presentations of paired odors (S1/S2) induces sensory preconditioning in MBON-γ1pedc>α/β.
(A) Upper part, diagram of experimental setup (odor schedule): preconditioning was induced by 10 repeated presentations of 4-methylcyclohexanol (MCH) and 3-octanol (OCT) (S1 and S2) with a 1-s …
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Figure 4—figure supplement 2—source data 1
Repeated presentations of paired odors (S1/S2) induces sensory preconditioning in MBON-γ1pedc>α/β.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig4-figsupp2-data1-v2.xlsx
Figure 4—figure supplement 3
TARGET temperature control for Rac1 inhibition does not show behavioral expression of sensory preconditioning.
(A) Diagram of experimental setup (odor schedule): Flies were kept at 18°C then transferred to 25°C before training. Flies were preconditioned by 10 repeated presentations of S1/S2 odor pairs with …
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Figure 4—figure supplement 3—source data 1
TARGET temperature control for Rac1 inhibition does not show behavioral expression of sensory preconditioning.
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig4-figsupp3-data1-v2.xlsx
Figure 4—figure supplement 4
Rac1 inhibition does not changes dynamics of calcium odor responses in γ lobes of Kenyon cells (KC).
Top part, diagram of the in vivo, under the microscope imaging setup. Rac1N17 was expressed in KC using R13F02-gal4>uas-rac1N17 using the temperature conditional system Target (tub-gal80ts). At the …
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Figure 4—figure supplement 4—source data 1
Rac1 inhibition does not changes dynamics of calcium odor responses in γ lobes of Kenyon cells (KC).
- https://cdn.elifesciences.org/articles/79107/elife-79107-fig4-figsupp4-data1-v2.xlsx
Videos
Video 1
Expression patterns of MB112C-splitGal4 driver expressing GFP.
Brains where dissected and stained using anti-GFP and anti-nc82 as a counterstain.
Video 2
Expression patterns of R12G04-LexA driver expressing GFP.
Brains where dissected and stained using anti-GFP and anti-nc82 as a counterstain.
Video 3
Expression patterns of R13F02-Gal4 driver expressing GFP.
Brains where dissected and stained using anti-GFP and anti-nc82 as a counterstain.
