Colour polymorphism associated with a gene duplication in male wood tiger moths
- University of Helsinki, Finland
- University of Cambridge, United Kingdom
- University of Oulu, Finland
- University of Jyväskylä, Finland
- Fujita Health University, Japan
Abstract
Colour is often used as an aposematic warning signal, with predator learning expected to lead to a single colour pattern within a population. However, there are many puzzling cases where aposematic signals are also polymorphic. The wood tiger moth, Arctia plantaginis, displays bright hindwing colours associated with unpalatability, and males have discrete colour morphs which vary in frequency between localities. In Finland, both white and yellow morphs can be found, and these colour morphs also differ in behavioural and life-history traits. Here, we show that male colour is linked to an extra copy of a yellow family gene that is only present in the white morphs. This white-specific duplication, which we name valkea, is highly upregulated during wing development. CRISPR targeting valkea resulted in editing of both valkea and its paralog, yellow-e, and led to the production of yellow wings. We also characterise the pigments responsible for yellow, white and black colouration, showing that yellow is partly produced by pheomelanins, while black is dopamine-derived eumelanin. Our results add to a growing number of studies on the genetic architecture of complex and seemingly paradoxical polymorphisms, and the role of gene duplications and structural variation in adaptive evolution.
Data availability
Scripts and data for the QTL, GWAS and DE analyses can be found at doi:10.5281/zenodo.8208751. RADseq, RNAseq data, and WGS of CRISPR samples were deposited to SRA under study accession number PRJNA937225. Raw sequencing data of wild samples has previously been deposited in ENA, study accession No. PRJEB36595.
Article and author information
Author details
Kazumasa Wakamatsu
Funding
Academy of Finland (343356)
- Melanie N Brien
Academy of Finland (345091 and 328474)
- Johanna Mappes
BBSRC (046_BB_V0145X_1)
- Chris D Jiggins
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2023, Brien et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 1,420
- views
-
- 203
- downloads
-
- 12
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 12
- citations for umbrella DOI https://doi.org/10.7554/eLife.80116
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Colour polymorphism associated with a gene duplication in male wood tiger moths
eLife 12:e80116.
https://doi.org/10.7554/eLife.80116
