The centrosomal protein 83 (CEP83) regulates human pluripotent stem cell differentiation towards the kidney lineage
- Charité-Universitätsmedizin Berlin, Germany
- Max Delbrück Center for Molecular Medicine, Germany
- University of Colorado Anschutz Medical Campus, United States
- Cairo University, Egypt
- Hannover Medical School, Germany
Abstract
During embryonic development, the mesoderm undergoes patterning into diverse lineages including axial, paraxial, and lateral plate mesoderm (LPM). Within the LPM, the so-called intermediate mesoderm (IM) forms kidney and urogenital tract progenitor cells, while the remaining LPM forms cardiovascular, hematopoietic, mesothelial, and additional progenitor cells. The signals that regulate these early lineage decisions are incompletely understood. Here, we found that the centrosomal protein 83 (CEP83), a centriolar component necessary for primary cilia formation and mutated in pediatric kidney disease, influences the differentiation of human induced pluripotent stem cells (hiPSCs) towards intermediate mesoderm. We induced inactivating deletions of CEP83 in hiPSCs and applied a 7 day in vitro protocol of intermediate mesoderm kidney progenitor differentiation, based on timed application of WNT and FGF agonists. We characterized induced mesodermal cell populations using single cell and bulk transcriptomics and tested their ability to form kidney structures in subsequent organoid culture. While hiPSCs with homozygous CEP83 inactivation were normal regarding morphology and transcriptome, their induced differentiation into IM progenitor cells was perturbed. Mesodermal cells induced after 7 days of monolayer culture of CEP83-deficient hiPCS exhibited absent or elongated primary cilia, displayed decreased expression of critical IM genes (PAX8, EYA1, HOXB7), and an aberrant induction of LPM markers (e. g. FOXF1, FOXF2, FENDRR, HAND1, HAND2). Upon subsequent organoid culture, wildtype cells differentiated to form kidney tubules and glomerular-like structures, whereas CEP83-deficient cells failed to generate kidney cell types, instead upregulating cardiomyocyte, vascular, and more general LPM progenitor markers. Our data suggest that CEP83 regulates the balance of intermediate mesoderm and lateral plate mesoderm formation from human pluripotent stem cells, identifying a potential link between centriolar or ciliary function and mesodermal lineage induction.
Data availability
Sequencing data have been deposited in GEO under accession code GSE205978.
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Parallel generation of easily selectable multiple nephronal cell types from human pluripotent stem cellsNCBI Gene Expression Omnibus, GSE75711.
Article and author information
Author details
Christian Mosimann
Funding
Deutsche Forschungsgemeinschaft (DFG; SFB 1365,GRK 2318,and FOR 2841))
- Kai M Schmidt-Ott
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Veronika Sander, University of Auckland, New Zealand
Version history
- Received: May 11, 2022
- Preprint posted: June 22, 2022 (view preprint)
- Accepted: October 11, 2022
- Accepted Manuscript published: October 12, 2022 (version 1)
- Version of Record published: November 2, 2022 (version 2)
Copyright
© 2022, Mansour et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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The centrosomal protein 83 (CEP83) regulates human pluripotent stem cell differentiation towards the kidney lineage
eLife 11:e80165.
https://doi.org/10.7554/eLife.80165
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