Immunofluorescent images (A, B) and quantification (C, D) of spinous (K10, gray) layer thickness in AGS3 KOs (A, C) and LGN KOs (B, D) compared to wild-type (WT) littermate controls. (E) Graphical depiction of clonal lineage tracing strategy using Krt14CreER and the multi-colored Rosa26confetti reporter, for data shown in (F–I). (F) Representative images of showing three types of clones. (G) Quantification of proportion of symmetric cell division (SCD) vs. asymmetric cell division (ACD) clones in AGS3 KOs (Gpsm1−/−) (blue) compared to heterozygote and WT (Gpsm1+/) controls (black). (H) Same analysis as (G) but for WT (black), LGN KO (Gpsm2−/−, red), and AGS3 + LGN dKO (Gpsm1−/−; Gpsm2−/−, magenta) clones. (I) Proportions of differentiative clones occurring by ACD vs. delamination for indicated genotypes. Scale bars: 50 µm in (A, B), 10 µm in (F); n values indicate measurements (C, D) or clones (G–I) from >3 independent embryos per genotype; n.s., not significant, **p < 0.01, ***p < 0.001 by unpaired t-test (C, D) or chi-square (G–I).