(A) PCA3 and PRUNE2 expression in nonneoplastic prostatic glandular tissue and in prostatic adenocarcinoma in the TCGA cohort illustrating consistent gene expression differences between tumor (n=497) and nonneoplastic (n=52) prostate in both cohorts. (B, C) PCA3 expression in the cohort from TCGA (n=497) across Gleason grades (B) showing lower expression in higher grade (>7) tumors and across tumor stages (C) showing lower expression in higher stage tumors. All illustrated tumor grades and stages show higher expression than nonneoplastic prostatic epithelium. (D) PCA3 expression in patients without versus with biochemical recurrence in the TCGA cohort (n=429). In the TCGA cohort, lower PCA3 median expression was associated with biochemical recurrence. Box plots of gene expression in the TCGA cohort is reported as log2RSEM data. The horizontal line within each box represents the median value, while the box represents the interquartile range, and the whiskers extend out to 1.5 times the interquartile range. Outliers are represented by circles. p-Values are noted for the indicated comparisons. RSEM: RNA-Seq by Expectation-Maximization.