A low-cost, open-source evolutionary bioreactor and its educational use
A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate Escherichia coli populations under chloramphenicol challenge, thereby enabling students to learn about bacterial growth and antibiotic resistance.
We provide the materials to build an EVE on our Github: https://github.com/vishhvaan/eve-pi. We have also included the data to generate figure 2 on the Github.
Article and author information
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Peter Rodgers, eLife, United Kingdom
- Preprint posted: August 9, 2019 (view preprint)
- Received: August 30, 2022
- Accepted: October 20, 2022
- Accepted Manuscript published: November 1, 2022 (version 1)
- Version of Record published: November 23, 2022 (version 2)
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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