The Arabidopsis SHORTROOT network coordinates shoot apical meristem development with auxin-dependent lateral organ initiation
- Institute for Developmental Genetics, Heinrich Heine University, Germany
- Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, Spain
- Laboratory of Plant Cell and Developmental Biology, Division of Biological and Environmental Sciences and Engineering, 4700 King Abdullah University of Science and Technology, Saudi Arabia
Figures
Figure 1 with 1 supplement
SHR and SCR functions modulate meristem size and auxin signalling in the shoot apical meristem.
(A–C) Top view of 21-day-old rosettes from WT (col-0) (A), shr-2 mutant (B) and scr-4 mutant (C). Scale bar represents 1 cm. (D) Mean flower plastochron in WT (n=21), shr-2 mutant (n=20) and scr-4 …
Figure 1—figure supplement 1
The shr and scr mutants phenotypes.
(A–D) Plant phenotypes of 42-day-old WT (n>30) (A), shr-2 mutant (n>35) (B), scr-3 mutant (n>30) (C) and scr-4 mutant (n>30) (D). Scale bars represent 1 cm. (A’-D’) Top view of 31-day-old …
Figure 2 with 2 supplements
Expression patterns of SHR and SCR in the shoot apical meristem and In vivo FRET–FLIM quantification of SHR–SCR association in the shoot apical meristem.
(A) Representative 3D projection of shoot apical meristem at 5 weeks after germination expressing pSHR:YFP-SHR reporter (green) (n≥6). The lower right inset shows a longitudinal optical section …
Figure 2—figure supplement 1
The expression pattern of SHR and SCR in the shoot apical meristem.
(A) Representative 3D projection of shoot apical meristem at 5 weeks after germination expressing pSHR:ntdTomato reporter (green). Cell walls were stained with DAPI (red) (n≥5). Scale bar represents …
Figure 2—figure supplement 2
SHR regulates SCR expression in the shoot apical meristem.
(A and B) Representative 3D projection of shoot apical meristem at 5 weeks after germination coexpressing pSHR:YFP-SHR reporter (green) and pSCR:SCR-RFP reporter (red) (A) (the lower left inset …
Figure 3 with 2 supplements
JKD functions and expression pattern in the shoot apical meristem.
SHR regulates CYCD6;1 expression in the shoot apical meristem. (A) Representative 3D projection of shoot apical meristems at 5 weeks after germination expressing pJKD:JKD-YFP reporter (green) …
Figure 3—figure supplement 1
The expression pattern of JKD in lfy and clv3 mutant shoot apical meristems and colocalization of the expression of JKD and SCR in the shoot apical meristem.
(A) Quantification of the epidermal cell area in the meristem region of WT (n=10) and jkd-4 mutant (n=10). (B–D) Representative 3D projection of shoot apical meristems at 5 weeks after germination …
Figure 3—figure supplement 2
The colocalization of the expression patterns of SHR, SCR, JKD and CYCD6;1 in the shoot apical meristem.
(A–D) Representative 3D projection of shoot apical meristem at 5 weeks after germination expressing pCYCD1;1-GFP reporter (green) (A), pCYCD3;2-GFP reporter (green) (B), pCYCD3;3-GFP reporter …
Figure 4 with 9 supplements
MP Regulates SHR and SCR expression in the shoot apical meristem.
(A) Quantitative real-time PCR analysis showing the relative expression levels of SHR and SCR expression in response to auxin (10 µm IAA) in WT shoot apical meristems. The expression level in Col-0 …
Figure 4—figure supplement 1
The expression patterns of SHR, SCR, MP and PIN1 in the shoot apical meristem.
(A and B) Representative 3D projection of shoot apical meristems at 5 weeks after germination coexpressing pSCR:SCR-YFP reporter (green) and pSHR:ntdTomato reporter (red) (A) and the pMP:MP-GFP …
Figure 4—figure supplement 2
CYCD6;1 expression responds to auxin.
(A and B) Representative 3D projection of shoot apical meristems at 5 weeks after germination expressing pSHR:SHR-YFP reporter (magenta) three days after mock (n≥6) (A) or 100 µM NPA treatment (n≥6) …
Figure 4—figure supplement 3
MP Regulates SHR and SCR expression in the shoot apical meristem.
(A and B) Representative 3D projection of shoot apical meristems at 7 days after germination expressing pSHR-ntdTomato reporter (magenta) in WT (n=4) (A) and mp-B4149 (n=4) (B) mutant vegetative …
Figure 4—figure supplement 4
shr mutant and shr mp-S319 double-mutant phenotypes.
(A–D) Plant phenotypes of 42-day-old WT (A), mp-S319 mutant (B), shr-2 mutant (C) and mp-S319 shr-2 double mutant (D). Scale bars represent 1 cm. (A’–D ’) Top view of 31-day-old inflorescences of WT …
Figure 4—figure supplement 5
MP induces the expression of SHR in planta.
(A) Schematic representation of the SHR promoter. The positions of two auxin response elements are shown. Overview of mutated promoter versions of pSHR. AuxREs were mutated and multiple combinations …
Figure 4—figure supplement 6
MP Regulates SHR expression in the shoot apical meristem.
(A) Schematic representation of the SHR promoter. The positions of two auxin response elements are shown. Overview of mutated promoter versions of pSHR. AuxREs were mutated and multiple combinations …
Figure 4—figure supplement 7
MP induces the expression of SHR in planta.
(A-H) Representative images of leaves transformed with pSHR variants pSHR (A and B), mAuxRE1 (C and D), mAuxRE2 (E and F) and mAuxRE1+2 (G and H) driving the reporter gene firefly luciferase (FLUC) …
Figure 4—figure supplement 8
The expression pattern of the different promoter versions of SHR in the root apical meristem.
(A–G) Representative images of root apical meristem at 5 days after germination expressing mVenus-NLS driven by the wild-type SHR promoter (A), and the SHR promoter with mutations in AuxRE motifs …
Figure 4—figure supplement 9
LFY act downstream of SHR in the shoot apical meristem.
(A) Representative 3D projection of shoot apical meristems at 5 weeks after germination coexpressing pLFY:LFY-GFP (green) and pSCR:SCR-RFP (red) reporters. Chlorophyll (blue). Scale bar represents …
Figure 5 with 4 supplements
Interplay of SCL23, SCR, SHR, SCL23-WUS Interaction in the Shoot Apical Meristem.
(A and B) Representative 3D projection of shoot apical meristems at 5 weeks after germination expressing pSCL23:H2B-YFP reporter (green) (n≥3) (A) and pSCL23:SCL23-YFP reporter (green) (n≥10) (B). …
Figure 5—figure supplement 1
SCL23 and WUS are negatively regulated by the CLV pathway in the shoot apical meristem.
(A–C) Representative 3D projection of shoot apical meristems at 5 weeks after germination from WT (A), scl23-2 mutant (B) and scl23-2/pSCL23:SCL23-YFP (C). Cell walls were stained with PI (gray). …
Figure 5—figure supplement 2
Mutant combinations of shr, scr, and scl23.
(A–H) Top view of 21-day-old rosettes of WT (A), scl23-2 mutant (B), shr-2 mutant (C), scr-3 mutant (D), scr-3 shr-2 double mutant (E), scl23-2 shr-2 double mutant (F), scl23-2 scr-3 double mutant (G…
Figure 5—figure supplement 3
Overexpression of SHR, SCR, and SCL23 in the SAM.
(A–D) Representative 3D projection of shoot apical meristems at 5 weeks after germination expressing p35S:SHR-GFP reporter (green) (A), p35S:SCR-GFP reporter (green) (B), pUBIQ10:SCL23-mVenus …
Figure 5—figure supplement 4
WUS and SCL23 cooperatively control shoot stem cell homeostasis in the shoot apical meristem.
(A–D) Top view of 21-day-old rosettes of Col-0 (A), scl23-2 mutant (B), wus-am mutant (C) and scl23-2 wus-am double mutant (D). Scale bar represents 1 cm. (E–I) plant phenotypes of 36 old L.er (E), …
Figure 6 with 1 supplement
Proposed model for SHR-SCR-SCL23-JKD regulatory network function in the SAM and the RAM.
(A and B) Schematic representation of observed expression patterns of WUS, SCL23, CLV3, JKD, SCR, LFY, SHR, MP and CYCD6;1 in the SAM (A) and WOX5, SCL23, JKD, SCR, SHR MP and CYCD6;1 in the RAM (B).…
Figure 6—figure supplement 1
Overlapping expression patterns of PIN1, MP, SHR, SCR, CYCD6;1, SCL23 and JKD in the RAM.
(A–D) Representative images of root apical meristem at 5 days after germination expressing, pPIN1:PIN1-GFP reporter (green) (A), pMP:MP-GFP reporter (green) (B), pSHR:YFP-SHR reporter (green) and pSC…
Tables
Table 1
Enzymes used in this study.
| Enzyme | Producer |
|---|---|
| BSA1 (ECORI) | Thermo Fisher Scientific, Braunschweig, Germany |
| Pfuµltra High-Fidelity DNA polymerase | Agilent, Santa Clara, USA |
| Phusion High-Fidelity DNA Polymerase | Thermo Fisher Scientific, Braunschweig, Germany |
| T4-Ligase | Thermo Fisher Scientific, Braunschweig, Germany |
| Taq-DNA-Polymerase | Made in the lab according to Pluthero, 1993 |
| Universal SYBR Green Supermix | Bio-Rad |
Table 2
Primers used for cloning.
| Purpose | Primer | Sequence |
|---|---|---|
| SHR promoter cloning | EB-pSHR-F | AAAGGTCTCAACCTGAAGCAGAGCGTGGGGTTTC |
| EB-pSHR-R | TTTGGTCTCATGTTTTTTAATGAATAAGAAAATGAATAGAAGAAAGGGGG | |
| EB-pSHR-BsaI-site-F | GTTCAAAAGTGGTCCCTTCTCTCTC | |
| EB-pSHR-BsaI-site-R | GAGAGAGAAGGGACCACTTTTGAAC | |
| SHR CDS cloning | EB-SHR-CDS-F | AAAGGTCTCAGGCTTAATGGATACTCTCTTTAGACTAGTCAG |
| EB-SHR-CDS-R | TTTGGTCTCACTGACGTTGGCCGCCACGCACTAG | |
| SCR CDS cloning | EB-SCR-CDS-F | AAAGGTCTCAGGCTTAATGGCGGAATCCGGCGATTTC |
| EB-SCR-CDS-R | TTTGGTCTCACTGAAGAACGAGGCGTCCAAGCTGAAG | |
| EB-SCR-CDS-BsaI-site-1-F | GCCATTATCAGGGACCTTATCC | |
| EB-SCR-CDS-BsaI-site-1-R | GGATAAGGTCCCTGATAATGGC | |
| EB-SCR-CDS-BsaI-site-2-F | GAAAATGGTATCTGCGTTTCAG | |
| EB-SCR-CDS-BsaI-site-2-R | CTGAAACGCAGATACCATTTTC | |
| JKD CDS cloning | EB-JKD-CDS-F | AAAGGTCTCAGGCTTAATGCAGATGATTCCAGGAGATCC |
| EB-JKD-CDS-R | TTTGGTCTCACTGAACCCAATGGAGCAAACCTTGCG | |
| EB-JKD-CDS-BsaI-site-F | GCCCTTGGTGACCTCACTGG | |
| EB-JKD-CDS-BsaI-site-R | CCAGTGAGGTCACCAAGGGC | |
| SCL23 CDS cloning | EB-SCL23-F | AAAGGTCTCAGGCTTAATGACTACAAAACGCATAGACAG |
| EB-SCL23-R | TTTGGTCTCACTGAATCGAACGGCTGAGATTTCC | |
| MP CDS cloning | EB-MP-GG-F | AAAGGTCTCAGGCTTAATGATGGCTTCATTGTCTT |
| EB-MP-GG-R | TTTGGTCTCACTGATGAAACAGAAGTCTTAAGATC | |
| pSHR site-directed mutagen-esis | EB-pSHRΔmAuxRE1-F | CTTTGTATCGAGCCAAACGAG |
| EB-pSHRΔmAuxRE1-R | CTCGTTTGGCTCGATACAAAG | |
| EB-pSHRΔmAuxRE2-F | TTCACATGGCTCTATGTTACTATG | |
| EB-pSHRΔmAuxRE1-R | CATAGTAACATAGAGCCATGTGAA | |
| EB-pSHRΔmAuxRE1-2-F | CTTTGTATCGAGCCAAACGAG | |
| EB-pSHRΔmAuxRE1-2-R | CTCGTTGTGCTCGATACAAAG | |
| EB-pSHRΔmAuxRE2-2-F | ATATTCACATGGGAGTATGTTACTATGTAAATG GTG ACC | |
| EB-pSHRΔmAuxRE2-2-R | GGTCACCATTTACATAGTAACATACTCCCATGTGAATAT |
Table 3
Primers used for qRT-PCR.
| Primer name | Sequence |
|---|---|
| EB-RT-SHR-F | GATATCGAGTTTCCGACGGT |
| EB-RT-SHR-R | CGAAGCAAACCCTAAACCAT |
| EB-RT-SCR-F | GTAACCCAAATCTCGGTGCT |
| EB-RT-SCR-R | TTGCTGTTGTGGAGGAGAAG |
| EB-RT-JKD-F | ATCAACCTGGCACTCCAGA |
| EB-RT-JKD-R | GCAGATCTCGCACACGAAT |
| EB-RT-LFY-F | TGATGCTCTCTCCCAAGAAGA |
| EB-RT-LFY-R | CTTGACCTGCGTCCCAGTA |
| EB-SAND-F | AACTCTATGCAGCATTTGATCCACT |
| EB-SAND-R | TGATTGCATATCTTTATCGCCATC |
| EB-TIP41-F | GTGAAAACTGTTGGAGAGAAGCAA |
| EB-TIP41-R | TCAACTGGATACCCTTTCGCA |
Table 4
Primers used for genotyping.
| Primer name | Sequence |
|---|---|
| EB-scl23-2-LP | ATGACTACAAAACGCATAGACAG |
| EB-scl23-2-RP | TTTGGTCTCACTGAATCGAACGGC |
| LBb1.3 | ATTTTGCCGATTTCGGAAC |
| mp-S319-LP | CCTGGAAACTGATGAGCTGAC |
| mp-S319-RP | CCTTCTTCACTCATCTGCTGG |
| LBb1.3 | ATTTTGCCGATTTCGGAAC |
| jkd-4-F | GGATGAAAGCAATGCAAAACA |
| jkd-4-R | AATGTCGGGATGATGAACTCC |
| RB | TCAAACAGGATTTTCGCCTGCT |
| scr-4F | CTGCTTCACCTACTGTATGGG |
| scr-4R | GGGTCAGAGGAAGAGGAAGG |
| Restriction enzyme: Eco57M which should not cut in the mutant | |
| shr-2-R | AAATCGAACTTGCGAATTCCT |
| shr-2-L | CGCTCAACGAGCTCTCTTCT |
| shr-2ins | CAGCAAGACAAGATGGGTCA |
| wus-7-F | CCGACCAAGAAAGCGGCAACA |
| wus-7-R | AGACGTTCTTGCCCTGAATCTTT |
| Restriction enzyme: XmNI which should cut in the mutant |
Table 5
Entry plasmids used in this study.
| Name | Description | Backbone | Reference |
|---|---|---|---|
| pSHR | SHR promoter 2.5 Kb upstream from transcription start | pBlunt | This study |
| SHR CDS | SHR coding sequence | pGGC000 | This study |
| SCL23 CDS | SCL23 coding sequence | pGGC000 | This study |
| linker NLS (pGGD007) | NUCLEAR LOCALIZATION SIGNAL | pGGD000 | Lampropoulos et al., 2013 |
| mVenus (pRD43) | mVenus | pGGD000 | Rebecca Burkhart |
| FLUC | Firefly luciferase | pGGC000 | Greg Denay |
| mVenus (pRD42) | mVenus | pGGC000 | Rebecca Burkhart |
| GUS (pGGC051) | coli ß-GLUCURONIDASE | pGGC000 | Lampropoulos et al., 2013 |
| MP CDS | MP coding sequence | pGGC000 | This study |
| 35 S promoter (pGGA004) | Cauliflower mosaic virus 35 S promoter | pGGA000 | Lampropoulos et al., 2013 |
| RPS5A promoter (pGGA012) | RIBOSOMAL PROTEIN 5 A promoter | pGGA000 | Lampropoulos et al., 2013 |
| UBIQ10 promoter (pGGA006) | UBIQUITIN10 promoter | pGGA000 | Lampropoulos et al., 2013 |
| d-dummy (pGGD002) | d-dummy | pGGD000 | Lampropoulos et al., 2013 |
| tCLV3 | CLV3 terminator 1257 bp downstream of transcription stop | pGGE000 | Jenia Schlegel |
| UBQ10 terminator (pGGE009) | UBQ10 terminator | pGGE000 | Lampropoulos et |
| BastaR (pGGF008) | pNOS:BastaR:tNOS | pGGF000 | Lampropoulos et al., 2013 |
| GR (pRD64) | Hormone-binding domain of the glucocorticoid receptor | pGGD000 | Rebecca Burkhart |
| pCLV3 | CLV3 promoter 1480 bp upstream from transcription start | pGGA000 | Jenia Schlegel |
| mega-element (pGGB002) | Omega- element | pGGB000 | Lampropoulos et al., 2013 |
Table 6
Destination plasmids used in this study.
| Name of construct | Promoter | N-Tag | CDS | C-Tag | Terminator | Resistance |
|---|---|---|---|---|---|---|
| pCLV3:SCL23-mVenus | pCLV3 | Ω- element (pGGB002) | SCL23 CDS | mVenus | tCLV3 | BastaR (pGGF008) |
| pRPS5A:SCL23-mVenus | pRPS5A | Ω- element (pGGB002) | SCL23 CDS | mVenus | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pUBIQ10:SCL23-mVenus | pUBIQ10 | Ω- element (pGGB002) | SCL23 CDS | mVenus | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pUBIQ10:MP-GR | pUBIQ10 | Ω- element (pGGB002) | MP CDS | GR | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| p35S:GUS | p35S | Ω- element (pGGB002) | GUS | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| p35S:MP | p35S | Ω- element (pGGB002) | MP CDS | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHR:mV-NLS | pSHR | Ω- element (pGGB002) | mVenus | linker NLS (pGGD007) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1:mV-NLS | pSHRΔmAuxRE1 | Ω- element (pGGB002) | mVenus | linker NLS (pGGD007) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE2:mV-NLS | pSHRΔmAuxRE2 | Ω- element (pGGB002) | mVenus | linker NLS (pGGD007) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1+2:mV-NLS | pSHRΔmAuxRE1+2 | Ω- element (pGGB002) | mVenus | linker NLS (pGGD007) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1-2:mV-NLS | pSHRΔmAuxRE1-2 | Ω- element (pGGB002) | mVenus | linker NLS (pGGD007) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE2-2:mV-NLS | pSHRΔmAuxRE2-2 | Ω- element (pGGB002) | mVenus | linker NLS (pGGD007) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1−2+2–2:mV-NLS | pSHRΔmAuxRE1−2+2–2 | Ω- element (pGGB002) | mVenus | linker NLS (pGGD007) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
Table 7
Destination plasmids used for luciferase assay.
| Name of construct | Promoter | N-Tag | CDS | C-Tag | Terminator | Resistance |
|---|---|---|---|---|---|---|
| pSHR:FLUC | pSHR | Ω- element (pGGB002) | FLUC | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1:FLUC | pSHRΔmAuxRE1 | Ω- element (pGGB002) | FLUC | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE2:FLUC | pSHRΔmAuxRE2 | Ω- element (pGGB002) | FLUC | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1+2:FLUC | pSHRΔmAuxRE1+2 | Ω- element (pGGB002) | FLUC | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1-2:FLUC | pSHRΔmAuxRE1-2 | Ω- element (pGGB002) | FLUC | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE2-2:FLUC | pSHRΔmAuxRE2-2 | Ω- element (pGGB002) | FLUC | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
| pSHRΔmAuxRE1−2+2–2:FLUC | pSHRΔmAuxRE1−2+2–2 | Ω- element (pGGB002) | FLUC | d-dummy (pGGD002) | tUBQ10 (pGGE009) | BastaR (pGGF008) |
Table 8
Mutants used in this study.
| Lines | Reference |
|---|---|
| scr-4 | Fukaki et al., 1998 |
| jkd-4 | Welch et al., 2007 |
| scr-3 | Fukaki et al., 1998 |
| shr-2 | Nakajima et al., 2001 |
| scl23-2 | Lee et al., 2008 |
| wus-7 | Graf et al., 2010 |
| wus-am | From Jan lohmann |
| mp-S319 | Schlereth et al., 2010 |
| mp-B4149 | Weijers et al., 2005 |
| lfy-12 | Huala and Sussex, 1992 |
| clv3-9 | Hobe et al., 2003 |
| cycd6;1 | GABI-Kat line (GK-368E07) Sozzani et al., 2010 |
Table 9
Transgenic lines used in this study.
| Lines | Plant Resistance | Reference |
|---|---|---|
| pSHR:mV-NLS | Basta | This study |
| pSHRΔmAuxRE1:mV-NLS | Basta | This study |
| pSHRΔmAuxRE2:mV-NLS | Basta | This study |
| pSHRΔmAuxRE1+2:mV-NLS | Basta | This study |
| pSHRΔmAuxRE1-2:mV-NLS | Basta | This study |
| pSHRΔmAuxRE2-2:mV-NLS | Basta | This study |
| pSHRΔmAuxRE1−2+2–2:mV-NLS | Basta | This study |
| pCLV3:SCL23-mVenus | Basta | This study |
| pRPS5A:SCL23-mVenus | Basta | This study |
| pUBIQ10:SCL23-mVenus | Basta | This study |
| pUBIQ10:MP-GR | Basta | This study |
| PlaCCI | Kanamycin | Desvoyes et al., 2020 |
| pSHR:mScarlet-SHR | - | This study |
| pSHR:YFP-SHR | Basta | Long et al., 2017 |
| pSCR:SCR-RFP | hyg | Long et al., 2017 |
| pSCR:SCR-YFP | basta | Long et al., 2017 |
| pSHR:SHR-YFP | Basta | Long et al., 2017 |
| pJKD:mRFP-YFP | norf | Long et al., 2017 |
| pJKD:JKD-YFP | Basta | Long et al., 2017 |
| pSCL23:SCL23-YFP | Kanamycin | Long et al., 2015a |
| pSCL23:H2B-YFP | Kanamycin | Long et al., 2015a |
| pCYCD6;1:GFP | Basta | Sozzani et al., 2010 |
| pSCR:H2B-YFP | Kanamycin | Heidstra et al., 2004 |
| pPIN1:PIN1-GFP | Kanamycin | Benková et al., 2003 |
| pSHR:nTdTOMATO | - | Möller et al., 2017 |
| pMP:MP-GFP | Kanamycin | Schlereth et al., 2010 |
| pLFY:LFY-GFP | kanamycin | Wu et al., 2003 |
| R2D2 | kanamycin | Liao et al., 2015 |
| pDR5v2:3xYFP-N7 | Basta | Heisler et al., 2005 |
| pWUS:3xVenus-NLS/pCLV3-mCherry-NLS | Kanamycin | Pfeiffer et al., 2016 |
| pCYCD1,1-GFP | - | Forzani et al., 2014 |
| pCYCD3,2-GFP | - | This study |
| pCYCD3,1-GFP | - | This study |
| pCYCD5,1-GFP | - | This study |
| pCYCD7,1-GFP | - | This study |
| pCYCD2,1-GFP | - | This study |
| pCYCD3,3-GFP | - | Forzani et al., 2014 |
| pCYCB1;1:CYCB1;1-GFP | Kanamycin | Ubeda-Tomás et al., 2009 |
