Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2
- University of California, Berkeley, United States
- California Institute for Quantitative Biosciences, United States
Abstract
Meiotic chromosome segregation relies on synapsis and crossover recombination between homologous chromosomes. These processes require multiple steps that are coordinated by the meiotic cell cycle and monitored by surveillance mechanisms. In diverse species, failures in chromosome synapsis can trigger a cell cycle delay and/or lead to apoptosis. How this key step in 'homolog engagement' is sensed and transduced by meiotic cells is unknown. Here we report that in C. elegans, recruitment of the Polo-like kinase PLK-2 to the synaptonemal complex triggers phosphorylation and inactivation of CHK-2, an early meiotic kinase required for pairing, synapsis, and double-strand break induction. Inactivation of CHK-2 terminates double-strand break formation and enables crossover designation and cell cycle progression. These findings illuminate how meiotic cells ensure crossover formation and accurate chromosome segregation.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting file; Source Data files have been provided for Figure 3-figure supplement 1 and Figure 3-figure supplement 2.
Article and author information
Author details
Funding
Howard Hughes Medical Institute
- Abby F Dernburg
National Institutes of Health (R01 GM065591)
- Abby F Dernburg
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Federico Pelisch, University of Dundee, United Kingdom
Version history
- Preprint posted: June 21, 2021 (view preprint)
- Received: October 26, 2022
- Accepted: January 25, 2023
- Accepted Manuscript published: January 26, 2023 (version 1)
- Version of Record published: March 9, 2023 (version 2)
Copyright
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2
eLife 12:e84492.
https://doi.org/10.7554/eLife.84492
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