Antagonistic role of the BTB-zinc finger transcription factors chinmo and broad-complex in the juvenile/pupal transition and in growth control
- Barcelona Institute of Science and Technology, Spain
- Institute of Evolutionary Biology (CSIC-UPF), Spain
Abstract
During development, the growing organism transits through a series of temporally regulated morphological stages to generate the adult form. In humans, for example, development progresses from childhood through to puberty and then to adulthood, when sexual maturity is attained. Similarly, in holometabolous insects, immature juveniles transit to the adult form through an intermediate pupal stage when larval tissues are eliminated and the imaginal progenitor cells form the adult structures. The identity of the larval, pupal and adult stages depends on the sequential expression of the transcription factors chinmo, Br-C and E93. However, how these transcription factors determine temporal identity in developing tissues is poorly understood. Here we report on the role of the larval specifier chinmo in larval and adult progenitor cells during fly development. Interestingly, chinmo promotes growth in larval and imaginal tissues in a Br-C-independent and -dependent manner, respectively. In addition, we found that the absence of chinmo during metamorphosis is critical for proper adult differentiation. Importantly, we also provide evidence that, in contrast to the well-known role of chinmo as a pro-oncogene, Br-C and E93 act as tumour suppressors. Finally, we reveal that the function of chinmo as a juvenile specifier is conserved in hemimetabolous insects as its homolog has a similar role in Blatella germanica. Taken together, our results suggest that the sequential expression of the transcription factors Chinmo, Br-C and E93 during larva, pupa an adult respectively, coordinate the formation of the different organs that constitute the adut organism.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting file. Source Data files have been provided for Figures 1 A and E (Figure1- SourceData1), Figure 2B, C, D and E (Figure2-SourceData1), Figure 5 B, C , D, E and F (Figure5-SourceData1), Figure 8G (Figure8-SourceData1), Figure 9 A and D (Figure9-SourceData1) and Figure2-figure suplement 1 D, E and F (Figure2-figure suplement 1-SourceData1).
Article and author information
Author details
Panagiotis Giannios
Xavier Franch-Marro
Funding
Ministerio de Ciencia e Innovación (PGC2018-098427-B-I00)
- David Martin
- Xavier Franch-Marro
Ministerio de Ciencia e Innovación (PID2021-125661NB-100)
- David Martin
- Xavier Franch-Marro
Ministerio de Ciencia e Innovación (PGC2018-094254-B-100)
- Jordi Casanova
Agència de Gestió d'Ajuts Universitaris i de Recerca (2017-SGR 1030)
- David Martin
- Xavier Franch-Marro
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2023, Chafino et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 2,002
- views
-
- 364
- downloads
-
- 12
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 12
- citations for umbrella DOI https://doi.org/10.7554/eLife.84648
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Antagonistic role of the BTB-zinc finger transcription factors chinmo and broad-complex in the juvenile/pupal transition and in growth control
eLife 12:e84648.
https://doi.org/10.7554/eLife.84648
