Activity of MukBEF for chromosome management in E. coli and its inhibition by MatP
eLife assessment
This important work combines DNA contact analysis and controlled genome rearrangements to investigate the processes that organize the E. coli chromosome, with a particular focus on how the SMC-related complex MukBEF is regulated. The evidence supporting the conclusions is compelling, with time-resolved experiments and analysis of mutant strains. The work will be of broad interest to chromosome biologists and bacterial cell biologists.
https://doi.org/10.7554/eLife.91185.3.sa0Important: Findings that have theoretical or practical implications beyond a single subfield
- Landmark
- Fundamental
- Important
- Valuable
- Useful
Compelling: Evidence that features methods, data and analyses more rigorous than the current state-of-the-art
- Exceptional
- Compelling
- Convincing
- Solid
- Incomplete
- Inadequate
During the peer-review process the editor and reviewers write an eLife Assessment that summarises the significance of the findings reported in the article (on a scale ranging from landmark to useful) and the strength of the evidence (on a scale ranging from exceptional to inadequate). Learn more about eLife Assessments
Abstract
Structural maintenance of chromosomes (SMC) complexes share conserved structures and serve a common role in maintaining chromosome architecture. In the bacterium Escherichia coli, the SMC complex MukBEF is necessary for rapid growth and the accurate segregation and positioning of the chromosome, although the specific molecular mechanisms involved are still unknown. Here, we used a number of in vivo assays to reveal how MukBEF controls chromosome conformation and how the MatP/matS system prevents MukBEF activity. Our results indicate that the loading of MukBEF occurs preferentially on newly replicated DNA, at multiple loci on the chromosome where it can promote long-range contacts in cis even though MukBEF can promote long-range contacts in the absence of replication. Using Hi-C and ChIP-seq analyses in strains with rearranged chromosomes, the prevention of MukBEF activity increases with the number of matS sites and this effect likely results from the unloading of MukBEF by MatP. Altogether, our results reveal how MukBEF operates to control chromosome folding and segregation in E. coli.
Introduction
Structural maintenance of chromosomes (SMC) complexes play key roles in many processes involved in chromosome management, from genome maintenance, interphase chromatin organization, sister chromatids alignment, chromosome folding and condensing, to DNA recombination at specific stages of the cell cycle (Yatskevich et al., 2019). A general model for SMC complex activity relies on their properties to bridge DNA elements and by doing so build DNA loops in cis and hold together the sister chromatids in trans; to do that, they bind DNA and processively extrude a DNA loop in an ATPase-driven loop extrusion, thereby compacting and organizing DNA (Davidson et al., 2019; Ganji et al., 2018; Kong et al., 2020). Yet many molecular features that determine the activity of the various SMC complexes are still unclear and it is not known whether they work using the same basal mechanisms (Bürmann et al., 2021; Davidson et al., 2019; Pradhan et al., 2022). For example, it is not yet clear whether (i) the activity of SMC complexes is mediated by a single complex or if it involves cooperation between several complexes that organize into dimers or even oligomers (Badrinarayanan et al., 2012b; Hassler et al., 2018), (ii) the DNA extrusion involves a topological or nontopological mechanism, i.e., does the DNA pass through the SMC ring topologically (Bürmann et al., 2021; Davidson et al., 2019; Pradhan et al., 2022), (iii) the activity of SMC complexes relies on the folding capacities of the SMC coiled-coil arms facilitating large-scale conformational changes (Bürmann et al., 2019), and (iv) multiple complexes that encounter one another on the same DNA in living cells bypass each other or collide (Anchimiuk et al., 2021; Brandão et al., 2021). Cohesin and condensin are the most characterized SMC complexes found in many eukaryotes. In bacteria, three different forms of SMC-like complexes defined as bacterial condensins have been identified, Smc-ScpAB, MukBEF, MksBEF; they are considered functionally related to condensins as they are thought to compact chromosomes and facilitate the segregation of sister chromosomes (Lioy et al., 2018; Lioy et al., 2020; Marbouty et al., 2015). In bacteria, Smc-ScpAB represents the most highly conserved complex, while MukBEF and MksBEF represent diverged SMC complexes (Cobbe and Heck, 2004; Yoshinaga and Inagaki, 2021).
Eukaryotic and prokaryotic SMC complexes are composed of at least five subunits: two Smc subunits, a kleisin subunit, and two additional subunits (referred to as kite and hawk subunits, depending on the type of SMC complex). Smc proteins associate with the kleisin protein to form a ring-shaped ATPase assembly. Additional subunits associate with this tripartite complex: the ‘Kite’ family associates with bacterial and archaeal SMC complexes and also with the eukaryotic SMC5/6 complex while the ‘Hawk’ family interacts with condensin and cohesin (Yatskevich et al., 2019). These additional subunits are thought to be required for the activity and to differentiate functions; for example, while condensin I and II share the same pair of Smc proteins, the difference in the subunit composition specifies their spatiotemporal dynamics and functional contributions to mitotic chromosome assembly (Hirota et al., 2004; Kong et al., 2020).
SMC function and dynamics on DNA requires additional auxiliary proteins (Baxter et al., 2019). More specifically, its loading on DNA and unloading of the DNA may depend on specific factors, at specific sites. For example, in Bacillus subtilis, the segregation ParB protein bound to parS site directly binds the Smc subunit; the ParB clamp presumably presents DNA to the SMC complex to initiate DNA loop extrusion (Gruber and Errington, 2009; Sullivan et al., 2009; Wang et al., 2015; Wang et al., 2017). Upon translocation, the site-specific recombinase XerD bound to its binding site unloads SMC complexes in the terminus region of the chromosome and this process is thought to involve specific interactions between the different components (Karaboja et al., 2021).
MukBEF was the first SMC complex identified. In Escherichia coli, MukBEF is thought to be required for chromosome segregation as muk mutants present many anucleate cells or mis-segregated chromosomes (Niki et al., 1991). How MukBEF may promote chromosome segregation and organization has remained elusive for a long time and is still not clear (Nolivos and Sherratt, 2014). The effect of the MukBEF complex in E. coli appears to be radically different from that of SMC in B. subtilis or other bacteria. Instead of aligning the chromosome arms from a centromere-like locus, MukBEF promotes DNA contacts in the megabase range within each replication arm (Lioy et al., 2018; Lioy et al., 2020). MukBEF promotes long-range interactions along the chromosome except in the 800-kb-long terminal domain (Ter) where MatP prevents its activity (Lioy et al., 2018). Under conditions of increased chromosome occupancy of MukBEF, the E. coli chromosome appears to be organized as a series of loops around a thin (<130 nm) MukBEF axial core (Mäkelä and Sherratt, 2020). Whether MukBEF is loaded at a particular locus is still an open question. The complete atomic structure of MukBEF in complex with MatP and DNA has been determined by electron cryomicroscopy (Bürmann et al., 2021); it also contains the MukBEF binding partner AcpP protein (Prince et al., 2021). It revealed that the complex binds two distinct DNA double helices reminiscent of the arms of an extruded loop, MatP-bound DNA threads through the MukBEF ring, while the second DNA is clamped by MukF, MukE, and the MukB ATPase heads. The presence of MatP in the complex together with its ability to prevent MukBEF activity prompted authors to propose that MatP might be an unloader of MukBEF (Bürmann et al., 2021).
Here, we have performed a number of experiments using different in vivo approaches to further characterize how MukBEF contributes to chromosome management in E. coli. We have used ChIP-seq and Chromosome Conformation Capture (3C-seq and Hi-C) experiments to study how MukBEF is loaded on the chromosome and promotes long-range DNA contacts. By using strains with various chromosome configurations obtained by programmed genetic rearrangements, we have explored how MukBEF activity proceeds along the chromosome and how MatP bound to matS sites prevents its activity. Our results together with comparative genomics analyses allow us to address the biological significance of multiple matS sites in the Ter region of chromosomes in enterobacteria and of the absence of MukBEF activity.
Results
MukBEF activity does not initiate at a single locus
In order to characterize how MukBEF interacts with the chromosome and initiate its activity of long-range contacts, it was necessary to set up a system to reveal using Hi-C the appearance and spread of long-range contacts along the chromosome upon MukBEF synthesis. The rationale was based on previous findings showing that long-range DNA contacts within replication arms, outside Ter, result from MukBEF activity. If MukBEF loads at a specific locus as observed for Smc-ScpAB at parS sites, we would expect to detect the appearance and spreading of long-range contacts from this site upon MukBEF synthesis. By contrast, if MukBEF loads stochastically or at multiple loci on the chromosome, long-range contacts should occur at multiple sites.
The analysis of MukBEF activity required an efficient system to control its activity. To conditionally synthesize MukBEF, the mukBEF operon was cloned onto a medium-copy number plasmid under control of a pLac promoter and introduced in a mukF mutant. In the absence of inducer, as observed for a mukF mutant, no growth was detected at 37°C and the amount of anucleated cells at 22°C was similar to that of the mukF mutant (13% vs 15%). Induced expression of mukBEF functionally complemented the absence of MukF, restoring growth at 37°C in Lennox Broth (LB) (Figure 1A) and accurately segregating the chromosome at 22°C, as evidenced by the low amount of anucleate cells (<2%) observed in the presence of the inducer (Figure 1B).
To determine how MukBEF activity initiates in the E. coli chromosome, we induced the expression of mukBEF and monitored the appearance of long-range DNA contacts at different times after induction (Figure 1). MukBEF synthesis was monitored by western blot (Figure 1—figure supplement 1D, E). Hi-C contact maps were established 20min, 40min, and 2 hr after induction and compared to that obtained in the absence of induction (Figure 1C with Figure 1—figure supplement 1B, C): long-range DNA contacts were hardly visible after 20 min (Figure 1C with Figure 1—figure supplement 1A), were readily observed after 40 min, and reached after 2 hr a level similar to that observed in wild-type (WT) strains (Figure 1 with Figure 1—figure supplement 1C). The ratio of normalized contact maps of the induced strain at different time points to the non-induced strain allowed to visualize the presence of long-range contacts all over the chromosome except in Ter (Figure 1D).
The range of DNA contacts along the chromosome was quantified by measuring the width of the diagonal perpendicular to it, using an adapted version of the quantification method developed before (Wang et al., 2017; Lioy et al., 2020). The plot obtained (Figure 1E) allows to estimate the effect of MukBEF activity on all loci of the E. coli genome. This confirmed the increase in the range of contacts following induction of MukBEF predicted from the ratio of normalized contact maps, with 40 min necessary to observe a significant effect and 2 hr to reach the maximum range of contact, as observed in the WT strain (Lioy et al., 2020; Figure 1 with Figure 1—figure supplement 1). As expected, Ter region was not affected by the induction of MukBEF because of the presence of MatP bound to matS sites.
Altogether, the results indicate that the increase of contacts does not originate from a specific position on the chromosome but rather appears from numerous sites, suggesting that the activity of MukBEF does not initiate at a single locus but rather from multiple loci along the chromosome.
MukBEF activity initiates in different regions of the E. coli genome
The MatP/matS system has been shown to prevent MukBEF activity in Ter (Lioy et al., 2018). We took advantage of this property to unravel mechanistic aspects of MukBEF activity and address the following issues: could MukBEF interacts with a region flanked by different Ter segments and could MukBEF operate from different regions of the chromosome. To answer these questions, we used bacteriophage λ site-specific recombination as described before (Thiel et al., 2012) to perform large transpositions of a segment of the right (‘RiTer’) and left (‘LiTer’) replichores at different loci (Figure 2—figure supplement 1A), in Ter, and analyze MukBEF activity in the resulting strain (Figure 2A).
The transposition in the RiTer11 configuration results in a region of 450 kb devoid of matS, located between two Ter segments of 378 and 437 kb, which contain 11 matS and 17 matS, respectively (Figure 2—figure supplement 1A). 3C-seq experiments were performed with this strain (‘RiTer11’) and on the same strain deleted for matP or for mukF (Figure 2A with Figure 2—figure supplement 1B). The ratio of normalized contact maps of the RiTer11 strain to the RiTer11 ΔmatP allowed to visualize the reduction of long-range contacts in the two Ter segments in the presence of MatP (Figure 2B). On the other hand, the ratio of normalized contact maps of the RiTer11 strain to the RiTer11ΔmukB allowed to visualize the increase of long-range contacts between and outside the two Ter segments in the presence of MukBEF. Remarkably, long-range DNA contacts specific of MukBEF activity were observed over the chromosome except in the two Ter segments. The range of DNA contacts along the chromosome was quantified by measuring the width of the diagonal in the two strains; these plots (Figure 2C) allow to estimate the effect of MukBEF activity on all loci inside and outside Ter segments. These results indicated that MukBEF can operate to form long-range contacts between the two Ter segments and that MatP can prevent MukBEF activity when segments carrying matS sites are moved in different locations of the genome.
Similar experiments were performed with a strain carrying a transposed segment from the left replichore in the Ter MD (LiTer 15). In this configuration, a region of 1085 kb devoid of matS is flanked by two Ter segments of 427 and 374 kb containing 13 matS and 15 matS, respectively (Figure 2 with Figure 2—figure supplement 1A). Hi-C experiments were performed on this strain (‘LiTer’) (Figure 2A). As observed with the RiTer11 strain, long-range DNA contacts specific of MukBEF activity were observed over the chromosome except in the two Ter segments. The ratio of normalized contact maps of the LiTer15 strain to the LiTer15 ΔmukB allowed to visualize the increase of long-range contacts outside the two Ter segments in the presence of MukBEF (Figure 2B and C with Figure 2—figure supplement 1B).
Altogether, these results demonstrate that MukBEF activity can be initiated from multiple regions of the chromosome, in the right, left, or Ori regions, and that Ter segments cannot insulate DNA segments devoid of matS sites. They also reveal that MukBEF does not globally translocate from the Ori region to the terminus of the chromosome as observed with Smc-ScpAB in different bacteria. Furthermore, the results indicated that Ter segments carrying 17 and 11 matS can prevent MukBEF activity and restrict DNA contacts.
matS determinants to prevent MukBEF activity
The method described above, splitting Ter in two parts, revealed that two parts of Ter can prevent MukBEF activity. Thus, by varying the way Ter is split in two parts, one should be able to identify matS determinants required to affect MukBEF activity.
Two previous studies differ slightly in the prediction for the number and consensus sequence of matS (Mercier et al., 2008; Nolivos et al., 2016). To clarify this, three independent ChIP-seq experiments were performed, revealing 28 matS and a newly derived consensus sequence (Figure 3—figure supplement 1). To examine the requirements for the inhibition of MukBEF by MatP, a 1 Mb region of the left replichore was transposed at different positions into the Ter domain, thus dividing Ter into two parts (referred to as Ter1 and Ter2, Ter1 comprising the dif site and the terminus of replication) of different sizes and carrying different number of matS (Figure 3A). Three transpositions were performed generating Ter2 domains of 253, 209, and 136 kb containing nine, seven, or four matS, respectively (strains LiTer9, LiTer7, LiTer4). We used Hi-C to test the ability of these Ter2 regions to inhibit MukBEF (LiTer9 in Figure 3—figure supplement 2, LiTer7 and LiTer4 in Figure 3B). Long-range DNA interactions were readily observed on both regions flanking Ter2 in both three strains, and long-range contacts are affected in the Ter2 segment, even when only four matS were present (Figure 3B). Similar Hi-C experiments were performed with the same strains deleted for matP (Figure 3—figure supplement 2B). The ratio of normalized contact maps of the LiTer4 strains to the corresponding ΔmatP derivatives (Figure 3C) clearly revealed that long-range contacts were limited in Ter2 of the different strains. Remarkably, the 136 kb region carrying four matS sites in LiTer4 was sufficient to decrease long-range contacts promoted by MukBEF activity.
The ability of different Ter segments to inhibit the formation of long-range DNA contacts was quantified by measuring the range of contacts in the different Ter segments (Figure 3D and E). The median range of contact for Ter in the WT condition is 263±24 kb. Remarkably, no significant differences in the range of contacts (275±9 kb) were observed in strain Liter9 for the Ter2 segment carrying nine matS; it is noteworthy that this region carrying nine matS sites shows the same range of contacts (280±35 kb) in the WT configuration. This result shows that a transposed 253 kb region carrying nine matS prevent MukBEF activity as much as the same segment present in the 838-kb-long Ter MD.
To further characterize how MatP/matS can prevent MukBEF activity, we measured the range of contacts in Ter2 segments carrying seven or four matS sites. For the 209 kb segment carrying seven matS sites, the range of contact was increased to 330±9 kb while it increased to 352±12 kb for the 136 kb segment with four matS. Values obtained for these segments when present as part of the WT Ter were slightly lower (Figure 3E). These results indicate that segments carrying seven or four matS affect MukBEF activity, even though at a lower level than a 250 kb segment with nine matS sites.
The four matS sites present in the 136 kb Ter2 region of LiTer4 are not distributed regularly in that region (Figure 3A). We took advantage of this irregular spacing to test the capacity of a segment carrying three matS sites to prevent MukBEF activity and to explore whether the density of matS sites can modulate MukBEF activity. To address these two questions, we deleted either matS26 or matS28 from strain LiTer4 and probed using Hi-C the long-range of contacts. In strain LiTer4 ΔmatS26, three matS sites are distant from 78 and 58 kb whereas the matS sites in LiTer4 ΔmatS28 strain are separated by 13 and 65 kb. The ratio of normalized contact maps of the LiTer4 ΔmatS26 or LiTer4 ΔmatS28 strains to the corresponding ΔmatP derivative (Figure 3B and C and Figure 3—figure supplement 2C) revealed that long-range contacts were affected by the presence of only three matS sites in the 136 kb segment. By measuring the range of contacts in strains LiTer4 ΔmatS26 and LiTer4 ΔmatS28 (Figure 3E), the density of three matS sites (matS25-matS26 and matS27) in strain LiTer4 ΔmatS28 seems to be as efficient (range contact of 357±11 kb) as the four matS sites in strain LiTer4 (range contact of 352±12 kb). By contrast, in LiTer4 ΔmatS26, the range of contact is increased to 370±6 kb indicating a reduced ability to prevent MukBEF activity. Altogether, assuming that differences in the matS sequences do not modify MatP’s ability to bind to the chromosome and affect its capacity to inhibit MukBEF, these results suggested that the density of matS sites in a small chromosomal region has a greater impact than dispersion of the same number of matS sites over a larger segment.
MukBEF preferentially binds in newly replicated regions
Results presented above indicated that MukBEF activity can be initiated from multiple regions of the chromosome and that, unlike Smc-ScpAB, MukBEF does not initiate its activity in the Ori region and translocate linearly to the terminus of the chromosome. To further characterize the loading and translocation process of MukBEF, we performed ChIP-seq experiments using a Flag version of MukB on synchronized cells using a dnaC2 thermosensitive allele that allows to control the timing of replication initiation (Figure 4A). The cells were grown at a permissive temperature, then shifted to 40°C for 2 hr to allow the ongoing round of replication to complete without being able to initiate a new round; the cells were then shifted back to 30°C and samples were taken for ChIP-seq analyses after 10, 20, and 40 min. At t0, there is no variation in the number of sequences along the chromosome indicating the absence of replication. In this condition, the ChIP-seq signals show a slight enrichment of signals outside Ter. After 10 min at the permissive temperature, a fraction of >500 kb centered on oriC was replicated as revealed by an increase of sequencing reads in this region. After 20 min, a large zone of over 1.4 Mb was replicated, and after 40 min, the chromosome was fully replicated and the replication profile was similar to that of non-synchronized cells because of multiple new replication cycles initiated. Normalized ChIP-seq experiments were performed by normalizing the quantity of immunoprecipitated fragments to the input of MukB-Flag. This experiment showed a two- to fourfold enrichment in the regions that have been replicated (Figure 4A with Figure 4—figure supplement 1). After 10 min, the signal was increased over 500 kb on each side of oriC. At 20 min, the signal progressed and corresponded to the regions that have been replicated. At 40 min, when the chromosome has been fully replicated, the signals obtained in the ChIP-seq samples indicated an enrichment of MukBEF all along the chromosome except in a 1.5 Mb region centered on the Ter region. As shown in Figure 4B, MukBEF enrichment drops to basal levels 250 kb before Ter (Figure 4A). This enrichment followed the progression of replication and spread from oriC toward Ter.
Altogether, these findings indicate that MukBEF is loaded into regions newly replicated either at the replication fork or even further behind it, except in the Ter region from which it would be excluded.
Ter segments prevent MukBEF binding
To further explore the ability of MatP to exclude MukBEF from the Ter region, we tested the ability of MatP to exclude MukBEF from chromosomal regions containing matS sequences. Chip-seq experiments were performed on the Liter7 strain, which has a Ter2 segment closer to oriC. As in the WT background, MukBEF is preferentially associated with newly replicated sequences and shows a twofold increase following replication fork progression. However, a break in this enrichment profile is detected in the sequence corresponding to Ter2 (Figure 4B). These results suggest that MukBEF does not bind or persist in segments carrying matS sites, and that the MatP/matS system prevent residence of MukBEF in that region.
MukBEF promotes long-range contacts in the absence of replication
Since MukBEF was shown to bind preferentially in newly replicated regions, we wanted to test if a preferential activity of MukBEF was detectable in those regions. To do this, we performed Hi-C on non-replicating cells lacking MukF, and induced mukBEF expression with or without restarting replication. In the absence of replication and of MukBEF, long-range contacts were constrained by barriers that delimit domains, called chromosome-interacting domains (CIDs) (Figure 4C with Figure 4—figure supplement 2), previously detected in WT cells in growing conditions (Lioy et al., 2018). Remarkably, after 2 hr of MukBEF induction in non-replicating cells, long-range contacts were detected except in the Ter region, indicating that MukBEF activity does not require newly replicated DNA to promote long-range contacts (Figure 4C, Figure 4—figure supplement 3). Finally, restarting replication for 40 min after 2 hr of MukBEF induction did not alter significantly the range and distribution of long-range DNA contacts observed in the absence of replication but in the presence of MukBEF outside Ter. As previously observed by microscopy in E. coli, replication does not appear to be an essential process for the activity of this SMC complex (Badrinarayanan et al., 2012a). Altogether, these results indicate that MukBEF promotes long-range DNA contacts independently of the replication process even though it binds preferentially in newly replicated regions.
Functional implications, comparative genomics of matS distribution
In E. coli, there are 28 matS sequences dispersed throughout the 1.03 Mb Ter domain. The average matS density is three matS per 100 kb, but this distribution is not uniform and the matS density doubles in the vicinity of the dif sequence (Figure 5 with Figure 5—figure supplement 1). Despite the fact that only nine matS are sufficient to completely inhibit MukBEF activity, matS sites have been selected and consequently a large portion of the E. coli chromosome is inaccessible to MukBEF.
To determine if other species also possess a large Ter domain, we used the matS consensus sequence to identify the Ter domain in 16 bacteria from the enterobacterales, pasteurellales, and vibrionales groups with the higher number of sequenced genomes. The Ter domain was defined as the longest stretch of matS sequences between areas that are at least 100 kb devoid of matS. The size of the Ter domain varies from 300 kb to 1 Mb, representing 6–25% of the chromosome, and contains 6–77 matS (Table 1). In most of these species, more than 14 matS sequences are distributed over 540 kb, and the Ter domain always contains the dif sequence. To test if the matS distribution might differ in the vicinity of the dif sequence, we measured the matS density and centered the distribution on the dif sequence. As shown in Figure 5, the number of matS per kb increases for all species and reaches its maximum near the dif site.
To determine if other species also possess a large Ter domain, we used the matS consensus sequence to identify the Ter domain in 16 bacteria from the enterobacterales, pasteurellales, and vibrionales groups with the higher number of sequenced genomes. The Ter domain was defined as the longest stretch of matS sequences between areas that are at least 100 kb devoid of matS. The size of the Ter domain varies from 300 kb to 1 Mb, representing 6–25% of the chromosome, and contains 6–77 matS (Table 1). In most of these species, more than 14 matS sequences are distributed over 540 kb, and the Ter domain always contains the dif sequence. To test if the matS distribution might differ in the vicinity of the dif sequence, we measured the matS density and centered the distribution on the dif sequence. As shown in Figure 5, the number of matS per kb increases for all species and reaches its maximum near the dif site.
Discussion
SMC complexes play a fundamental role in the organization of genomes in all domains of life. Different models involving their loading, translocation, or extrusion of DNA loops as well as their unloading have been proposed, based on results obtained with different complexes and in different models. Among the SMC complexes, MukBEF has specific features: MukBEF is only found in enterobacteria and some related bacterial genera where it is involved in chromosome segregation; the mukBEF genes belong to a group of genes co-occurring with the Dam methylase gene including also matP and other genes involved in DNA metabolism (Brézellec et al., 2006); MukBEF exists as dimers of dimers connected by the kleisin subunit MukF (Badrinarayanan et al., 2012b; Zawadzka et al., 2018); by its activity, MukBEF does not align the two arms of the chromosome like the canonical bacterial Smc-ScpAB complex but instead promotes long-distance contacts in cis like a eukaryotic condensin (Lioy et al., 2018). Remarkably, MukBEF activity is not detected within the specific chromosomal Ter domain, one-fifth of the E. coli chromosome, due to the presence of MatP associated with this region. Although details about MukBEF and its activity have been revealed in recent years, key steps in its function remain to be characterized, including loading onto the DNA molecule, its actual loop extrusion activity, and its unloading by MatP.
Altogether, our data provide an integrated view of MukBEF activity to organize the E. coli chromosome. By different ChIP-seq and Hi-C approaches performed in different strains, some of which have undergone programmed genetic rearrangements, we showed that MukBEF loading does not only involve the Ori region but also different regions of the chromosome. Our results also indicated that although MukBEF binds preferentially in newly replicated regions, its activity is detected even in the absence of replication and long-range contacts appear similarly throughout the genome, except in the Ter region. These results support a model in which MukBEF molecules are bound to the chromosome, molecules are removed or displaced by replication, MukBEF molecules readily reassociate in newly replicated regions except in Ter region in which the unloading of MukBEF is enhanced by MatP bound to matS sites. Our results are in agreement with the previous proposal that MukBEF may organize a series of loops around a thin MukBEF axial core (Mäkelä and Sherratt, 2020). Altogether, our results reveal a striking contrast with the way Smc-ScpAB loads on DNA by interacting with the ParB at parS sites and then translocates toward the ter region (Wang et al., 2017). Instead, long-distance contacts promoted by MukBEF did not occur as a wave from a specific region but rather initiate at different positions, in different regions of the genome. Thus, the activity of MukBEF appears to be more similar to that of eukaryotic condensins than to that of the Smc-ScpAB complex.
The fact that MukBEF is found associated with newly replicated DNA could also suggest preferential loading at the replication fork or behind. However, in the absence of replication, MukBEF is still able to load and form long-range DNA contacts, as well as segregated chromosomes (Badrinarayanan et al., 2012a). Interestingly, this situation is reminiscent to that of SMC-ScpAB. Smc-ScpAB is loaded onto the B. subtilis chromosome at the parS site in association with parB/SpoJ (Gruber and Errington, 2009; Sullivan et al., 2009). Remarkably, in the absence of parB, Smc-ScpAB is still able to perform chromosome segregation almost perfectly (Ireton et al., 1994; Wang et al., 2014). This demonstrates that, even with a strong preferential loading mechanism, the SMC complex can load on chromosomal DNA. A similar situation may occur in E. coli, where MukBEF is preferentially loaded depending on the progression of replication but can still bind to and act on the chromosome in the absence of replication.
Our results showed that MukBEF was not detected in regions containing matS sites and that these regions were devoid of contacts extending over 600 kb. By varying the number of matS sites at an ectopic position, we showed that MukBEF inhibition is graded with the number of matS; while an effect is already visible with three matS scattered over a 78 kb region, the maximal effect seems to be reached with nine matS sites distributed over a 253 kb region. As proposed for Smc-ScpAB and its unloading XDS site (Karaboja et al., 2021), if the movement of DNA in the loop extrusion process involves large steps, it is conceivable that several matS sites are required for a complex to be trapped and discharged by MatP bound to a matS site. Accordingly, the density of matS sites seems to affect the inhibition efficiency supporting this assumption. Further experiments will be needed to analyze in detail the optimal spacing of matS sites to inhibit MukBEF activity.
The structure of the MukBEF-AcpP-MatP/matS complex obtained by cryoEM revealed the entrapment of two topologically separated DNA segments in two distinct compartments called ‘ring’ and ‘clamp’, with the matS site present in the ring compartment. The proposed model for MukBEF unloading stipulates that the unloading of the segment carrying matS site in the ring compartment is coupled to the unloading of the other segment in the clamp. One of the MatP monomers forms a contact with one of the MukE monomers while the joint binds and positions MatP between the MukB arms. The joint interface is much larger than the MukE-MatP bridge and likely provides the major binding energy for association (Bürmann et al., 2021). Indeed, a change to alanine of the five residues between H38 and D42 in contact with MukE did not affect MatP’s ability to inhibit MukBEF activity (Figure 3—figure supplement 3). Refined experiments will be required to assess the outcome of mutating MatP residues involved in the interaction with the joint as mutations of those residues also affect matS binding (Dupaigne et al., 2012).
MukBEF activity is detected by the appearance of contacts over a distance of approximately 1 Mb, spanning around 4 Mb of the chromosome (excluding the Ter domain). Even if the molecular details of this activity remain to be characterized, it is tempting to speculate that MukBEF molecules can be discharged from DNA in the absence of MatP, i.e., outside Ter. Furthermore, it can be noted that MukBEF activity does not appear to be significantly disrupted in a matP mutant, suggesting that MukBEF can be discharged from DNA in the absence of MatP. As proposed by Bürmann et al., 2021, MatP would act as a structural element that ensures ideal positioning of DNA close to the exit gate in the MukBEF complex that might ensure an efficient unloading of MukBEF from the DNA. Altogether, the results would indicate that MatP is not the MukBEF unloader per se, but rather that its ability to prevent MukBEF activity has been selected to protect the Ter region from a condensin activity.
The distribution of matS sites over a large region of the chromosome in enterobacteria results in the inhibition of MukBEF in Ter. MatP has already been shown to confer another property to Ter, through an interaction of its C-terminus with the septum-associated protein ZapB and localizing Ter at midcell. Remarkably, these two properties are independent as inhibiting MukBEF does not require its anchoring of Ter at the septum of division (Lioy et al., 2018). Given that the number of matS sites far exceeds the number required to prevent MukBEF activity and that the density of matS sites increases as we approach dif, we may speculate that MukBEF presence is mostly banished from the dif region. At least two activities of DNA metabolism occur in this region: resolution of chromosome dimers by XerC-XerD recombinases and the post-replicative decatenation of circular chromosomes. We may speculate, based on in vitro observations (Kumar et al., 2022), that MukBEF could interfere with TopIV activity and delay potential chromosome decatenation. Another possibility is that chromosome dimers resolved at the dif site may become trapped in loops formed by MukBEF, thus delaying segregation. However, none of these possible scenarios are supported by data yet, and a major challenge for the future will be to determine whether and how MukBEF may interfere with one or both of these processes.
In the absence of replication and of MukBEF activity, the contact map displayed a single strong diagonal composed of very well-defined CIDs, ranging in size from 20 to 400 kb. The CIDs, clearly visible in populations of cells growing exponentially, were prominent in these conditions revealing the impact of transcription on the structuring of the genome. Upon replication, the CID organization is less apparent because of the new contacts that occur between loci belonging to different CIDs. The induction of MukBEF in the presence of replication further attenuates the patterning of CIDs. Altogether, these results highlight the respective contribution of these three processes, transcription, replication, and condensin activity, on the organization of bacterial genomes.
Materials and methods
Bacterial strains and plasmids
Request a detailed protocolAll E. coli strains used in this study are derived from E. coli MG1655. Deletion mutants were constructed as described in Datsenko and Wanner, 2000. Mutations were combined via P1 transduction. A plasmid capable of synthesizing MukBEF was constructed by cloning the entire mukBEF operon into the pPSV38 plasmid using EcoRI/XbaI. MukBEF synthesis was monitored by western blot after the addition of IPTG at 0, 20, 40, and 120 min (Figure 1—figure supplement 1D).
Media and growth conditions
Request a detailed protocolE. coli cells were cultured at 22°C and 30°C in either LB or liquid minimal medium A (MM) supplemented with 0.12% casamino acids and 0.4% glucose. When necessary, antibiotics were added to the growth media at the following concentrations: ampicillin at 100 μg/ml, kanamycin at 50 μg/ml, chloramphenicol at 15 μg/ml, spectinomycin at 50 μg/ml, apramycin at 50 μg/ml, and zeocin at 25 μg/ml.
3C-seq protocol
Request a detailed protocol3C-seq libraries were generated as described (Lioy et al., 2018). Briefly 100 ml of culture were crosslinked with formaldehyde (7% final concentration) for 30 min at room temperature followed by 30 min at 4°C. Formaldehyde was quenched with a final concentration of 0.25 M glycine for 20 min at 4°C. Fixed cells were collected by centrifugation and stored at −80°C until use. Frozen pellets consisting of approximately 1–2 × 109 cells were thawed and suspended in 600 μl of TE buffer (10 mM Tris-HCl, 0.5 mM EDTA, pH 8) with 4 μl of lysozyme (35 U/μl). The mixture was incubated at room temperature for 20 min. Subsequently, SDS was added to a final concentration of 0.5% and the cells were incubated for an additional 10 min at room temperature. Lysed cells were then diluted 10 times in several tubes containing 450 μl of digestion mix (1× NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hr at 37°C. To stop the digestion reaction, the mixture was centrifuged for 20 min at 20,000 × g, and the resulting pellets were resuspended in 500 μl of sterile water. The resulting digested DNA (4 ml in total) was divided into four aliquots and diluted in 8 ml of ligation buffer (1× ligation buffer NEB without ATP, 1 mM ATP, 0.1 mg/ml BSA, 125 units of T4 DNA ligase, 5 U/μl). Ligation was performed at 16°C for 4 hr, followed by overnight incubation at 65°C with 100 μl of proteinase K (20 mg/ml) and 100 μl EDTA 500 mM. DNA was then precipitated with an equal volume of 3 M Na-acetate (pH 5.2) and two volumes of iso-propanol. After 1 hr at –80°C, the DNA was pelleted and suspended in 500 μl of 1× TE buffer. The tubes were incubated directly with 50 μl of proteinase K (20 mg/ml) for an overnight period at 65°C. Subsequently, all tubes were transferred to 2 ml centrifuge tubes and extracted with 400 μl of phenol-chloroform pH 8.0. The DNA was then precipitated, washed with 1 ml of 70% cold ethanol, and resuspend in 30 μl of 1× TE buffer in the presence of RNase A (1 μg/ml). The tubes containing ligated DNA (3C libraries), digested DNA, and non-digested DNA were pooled into three separate tubes. The efficiency of the 3C preparation was evaluated by running a 1% agarose gel.
Hi-C protocol
Request a detailed protocolHi-C libraries were generated as described (Thierry and Cockram, 2022).
108 cells growing in the exponential growth phase of E. coli were chemically crosslinked by the addition of formaldehyde directly to the cultures (3% final concentration) for 30 min at room temperature with gentle agitation. Crosslinking was quenched by the addition of glycine (0.5 M final concentration) for 20 min at room temperature. Cells were washed in 50 ml PBS 1× and centrifuged. The pellet was resuspended in 1 ml 1× PBS and transferred to a 1.5 ml Eppendorf tube before a final centrifugation step (4000 × g, 5 min, room temperature), the supernatant was then removed and the pellet stored at −80°C. The pellet was then resuspended in 1 ml of 1× TE + complete protease inhibitor cocktail (EDTA-free, Sigma-Aldrich) and transferred to a 2 ml Eppendorf tube with 4 µl of ready-to-lyse lysozyme for 20 min at room temperature. SDS was added to a final concentration of 0.5% and the cells were incubated for an additional 10 min. In a 10 ml Falcon tube, DNA was then prepared for digestion by the addition of 3 ml H2O, 500 μl 10× Digestion buffer (200 mM Tris-HCl pH 7.5, 100 mM MgCl2, 10 mM DTT, 1 mg/ml BSA) and 500 μl 10% Triton X-100 (Thermo Fisher). After thoroughly mixing the reaction, 400 μl were removed and transferred to a 1.5 ml Eppendorf tube as a non-digested (ND) control. The restriction enzyme HpaII (New England Biolabs, 1000 U) was then added to the remaining sample and the tube incubated with gentle agitation for 3 hr at 37°C. The solution was centrifuged at 16,000 × g, 20 min, room temperature. After removing the supernatant, the pellet was resuspended in 400 μl H2O, completed subsequently by adding 50 μl 10× Ligation Buffer (500 mM Tris-HCl pH 7.5, 100 mM MgCl2, 100 mM DTT), 4.5 μl 10 mM dAGTTp, 37.5 μl Biotin-14-dCTP (Thermo Fisher), 50 units of DNA Polymerase I - Large Klenow Fragment (New England Biolabs). After briefly mixing, the reaction was incubated with agitation for 45 min at 37°C. The ligation was set up by adding the following: 120 μl 10× Ligation Buffer, 12 μl 10 mg/ml BSA, 12 μl 100 mM ATP, 540 μl H2O, 480 U T4 DNA ligase (Thermo Fisher). The reaction was mixed gently and then incubated with gentle agitation for 3 hr at room temperature. Following ligation, proteins were denatured by the addition of 20 μl 500 mM EDTA, 20 μl 10% SDS, and 100 μl 20 mg/ml proteinase K (EuroBio). The following day, DNA was purified using the standard procedure described for the 3C-seq.
Chip-seq protocol
Request a detailed protocol109 cells in the exponential growth phase of E. coli were chemically crosslinked by adding formaldehyde directly to the cultures (final concentration of 1%) for 30 min at room temperature with gentle agitation. Crosslinking was quenched by adding glycine (final concentration of 0.25 M) for 15 min at room temperature. The cells were washed twice with 1× TBS (50 mM Tris-HCl pH 7.6, 0.15 M NaCl) and the pellet was resuspended in 1 ml of 1× TBS before a final centrifugation step (4000 × g, 5 min, room temperature). The supernatant was then removed and the pellet was stored at –80°C.
The pellet was resuspended in 500 µl of lysis buffer 1 (20% sucrose, 10 mM Tris pH 8, 50 mM NaCl, 10 mM EDTA) and 4 µl of ready-to-lyse lysozyme was added, followed by incubation at 37°C for 30 min. Then, 500 µl of lysis buffer 2 (50 mM Tris-HCl pH 4.7, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) and a tablet of antiprotease cocktail (Roche) were added. The solution was transferred to a 1 ml Covaris tube and sonicated for 10 min (peak incident power 140 W/duty cycle 5%/cycle per burst 200). Cell debris were eliminated by centrifugation at 13,000 rpm for 30 min at 4°C. The supernatant was transferred to a fresh Eppendorf tube and 50 µl was used as input. The rest of the cell extract was mixed with 40 µl of anti-Flag M2 resin previously washed in TBS and resuspended in lysis buffer 2. The solution was incubated overnight at 4°C with gentle mixing and then centrifuged for 30 s at 5000 × g. The pellet was washed twice with TBS containing 0.05% Tween 20 and three times with TBS.
To elute the immunoprecipitation, 100 µl of 1× TBS containing 15 µg of 3× Flag peptide was mixed with the resin and incubated for 30 min at 4°C. Then, the solution was centrifuged for 30 s at 5000 × g and the supernatant was transferred to a new tube. A second elution step was performed on the resin before decrosslinking. IP and input was purified using MinElute QIAGEN columns and then sequenced.
Processing of libraries for Illumina sequencing
Request a detailed protocolThe samples were sonicated using a Covaris S220 instrument to obtain fragments ranging in size from 300 to 500 base pairs. These fragments were then purified using AMPure XP beads and resuspended in 10 mM Tris-HCl. For Hi-C libraries, a biotinylated pull-down step was performed by adding 30 µl of streptavidin C1 MyOne Dynabeads from Invitrogen to 300 µl of binding buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 2 M NaCl) and mixing with the Hi-C libraries for 15 min. DNA ends were then prepared for adaptor ligation following standard protocols as described in Thierry and Cockram, 2022.
The Illumina sequencing process was performed in accordance with the manufacturer’s recommendations, with 15 cycles of amplification. The size of the DNA fragments in the libraries was assessed using TAE 1% agarose gel and tape station, followed by paired-end sequencing on an Illumina sequencer.
Chip-seq analysis
Request a detailed protocolBetween 10 and 20 million reads were recovered for each sample. We used Bowtie2 software to perform mapping in local mode, and the mpileup software from Samtools to calculate coverage for each position in the genome. Normalized ChIP was then calculated by normalizing the number of reads at each position by the total number of reads, and dividing this number by the normalized input. A sliding window of 50 kb was applied to smooth the variations. Peaks for MatP ChIP-seq (Figure 3—figure supplement 1B) were identified by extracting positions where the number of reads was 10 times higher than the background for at least 30 consecutive base pairs. The center of these distributions was considered the center of the peak. The sequences were then extracted and used on the MEME suite to identify a common motif.
Generation of contact maps
Request a detailed protocolContact maps were constructed as described previously (Lioy et al., 2018). Briefly, each read was assigned to a restriction fragment. Non-informative events such as self-circularized restriction fragments, or uncut co-linear restriction fragments were discarded, as in Cournac et al., 2012. The genome was then divided into 5 kb units, and the corresponding contact map was generated and normalized through the sequential component normalization procedure of SCN (based on the sequential component normalization; https://github.com/koszullab/E_coli_analysis; Koszul, 2020; Lioy et al., 2018). Contact maps were visualized as logarithmic matrices to aid in visualization.
Ratio of contact maps
Request a detailed protocolThe comparison of contact maps was facilitated by displaying their ratio. The ratio was calculated for each point on the map by dividing the number of contacts in one condition by the number of contacts in the other condition. The Log2 of the ratio was then plotted using a Gaussian filter. The color code represented a decrease or increase in contacts in one condition relative to the other (a blue or red signal, respectively); a white signal indicated no change.
Quantification of the range of cis contacts along the chromosome
Request a detailed protocolWe used a three-step process adapted from Lioy et al., 2020, and Wang et al., 2017, to determine the width of the diagonal in contact maps. To improve the resolution of the Ter limit, we measured the width perpendicular to the main diagonal.
First, we calculated the median of the contact map and estimated the standard deviation (σ) using a robust statistic, where σ=1.4826 * mad (mad = median absolute deviation). Next, we used a point connecting algorithm to differentiate significant interactions from background noise. The size of each connected element identified by the ‘bwlabel’ function of MATLAB was determined. We considered a connected element with a size ≥30 points to be significant and used the ‘imclose()’ function of MATLAB to fill in the empty points within the connected elements using a diamond shape with a size of 5.
Subsequently, we calculated the width of the primary diagonal for each 5 kb bin of the genome. The range of cis contact was estimated from the width of the primary diagonal by multiplying the number of measured bins by the bin size (5 kb) and dividing by two (since the range is symmetric on both sides of the chromosomal locus being considered). Finally, a boxplot was used to visualize the entire range of cis contacts for all the analyzed chromosome regions.
Directional index analysis
Request a detailed protocolDirectional index was calculated as described in Lioy et al., 2018. The directional index is a statistical metric that quantifies the level of upstream or downstream contact bias for a given genomic region (Dixon et al., 2012). This metric is based on a t-test comparison of contact vectors to the left and right of each bin, up to a certain scale. The boundaries between topological domains often generate fluctuating signals that result in a transition in the directional preference. Specifically, for each 5 kb bin, we extracted the contact vector from the correlation matrix between that bin and neighboring bins at regular 5 kb intervals, up to 100 kb, in both left and right directions. At each step, the paired t-test was used to determine whether the strength of interactions was significantly stronger in one direction relative to the other. A threshold of 0.05 was used to assess statistical significance. The directional preferences for each bin along the chromosome were visualized as a bar plot, with positive and negative t-values shown as red and green bars, respectively. To improve the clarity of presentation, bars for bins with t-values below –2 or above 2 (corresponding to a p-value of 0.05) were truncated. Between two identified domains in the contact matrices, the directional preference of bins changed, which was indicated by alternating red and green colors.
Data availability
The DNA sequencing raw data was deposited on the NCBI website under the bioproject numbers: PRJNA1019269 (HiC induction of MukBEF), PRJNA1021387 (HiC on transposed strains), PRJNA1024671 (HiC on non-replicating strains), and PRJNA1024669 (ChIP-seq of MukB on synchronized strains).
-
NCBI BioProjectID PRJNA1019269. HiC Ecoli MukBEF induction.
-
NCBI BioProjectID PRJNA1021387. HiC transposed Ter strain.
-
NCBI BioProjectID PRJNA1024671. HiC +/- repliction.
-
NCBI BioProjectID PRJNA1024669. MukB ChIP-seq on synchronized cells.
References
-
The Escherichia coli SMC complex, MukBEF, shapes nucleoid organization independently of DNA replicationJournal of Bacteriology 194:4669–4676.https://doi.org/10.1128/JB.00957-12
-
DNA-loop-extruding SMC complexes can traverse one another in vivoNature Structural & Molecular Biology 28:642–651.https://doi.org/10.1038/s41594-021-00626-1
-
A folded conformation of MukBEF and cohesinNature Structural & Molecular Biology 26:227–236.https://doi.org/10.1038/s41594-019-0196-z
-
The evolution of SMC proteins: phylogenetic analysis and structural implicationsMolecular Biology and Evolution 21:332–347.https://doi.org/10.1093/molbev/msh023
-
Towards a Unified Model of SMC Complex FunctionCurrent Biology 28:R1266–R1281.https://doi.org/10.1016/j.cub.2018.08.034
-
Distinct functions of condensin I and II in mitotic chromosome assemblyJournal of Cell Science 117:6435–6445.https://doi.org/10.1242/jcs.01604
-
The MukB-topoisomerase IV interaction mutually suppresses their catalytic activitiesNucleic Acids Research 50:2621–2634.https://doi.org/10.1093/nar/gkab1027
-
High-resolution mapping of the spatial organization of a bacterial chromosomeScience 342:731–734.
-
The bacterial chromosome: architecture and action of bacterial SMC and SMC-like complexesFEMS Microbiology Reviews 38:380–392.https://doi.org/10.1111/1574-6976.12045
-
BookGenerating high-resolution Hi-C contact maps of bacteriaIn: Bicciato S, Ferrari F, editors. Hi-C Data Analysis, Methods in Molecular Biology. Springer US. pp. 183–195.https://doi.org/10.1007/978-1-0716-1390-0_9
-
Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtilisGenes & Development 29:1661–1675.https://doi.org/10.1101/gad.265876.115
-
Organization of Chromosomal DNA by SMC ComplexesAnnual Review of Genetics 53:445–482.https://doi.org/10.1146/annurev-genet-112618-043633
-
Ubiquity and Origins of Structural Maintenance of Chromosomes (SMC) Proteins in EukaryotesGenome Biology and Evolution 13:evab256.https://doi.org/10.1093/gbe/evab256
Article and author information
Author details
Funding
Agence Nationale de la Recherche (ANR-CE12-0013-01)
- Frederic Boccard
Fondation ARC pour la Recherche sur le Cancer (PJA 20181208006)
- Mohammed Seba
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank members of the FB laboratory for help and fruitful discussions. We also thank the I2BC NGS facility for high-throughput sequencing. This project was supported by grants from 'Agence Nationale de la Recherche' (ANR-CE12-0013-01) and the 'Association pour la Recherche contre le Cancer' (Projet Fondation ARC 2018). MS was supported by a doctoral fellowship from CNRS and from the 'Association pour la Recherche contre le Cancer'.
Version history
- Sent for peer review:
- Preprint posted:
- Reviewed Preprint version 1:
- Reviewed Preprint version 2:
- Version of Record published:
- Version of Record updated:
Cite all versions
You can cite all versions using the DOI https://doi.org/10.7554/eLife.91185. This DOI represents all versions, and will always resolve to the latest one.
Copyright
© 2023, Seba et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 763
- views
-
- 87
- downloads
-
- 5
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Further reading
-
- Biochemistry and Chemical Biology
- Chromosomes and Gene Expression
The mRNA 5'-cap structure removal by the decapping enzyme DCP2 is a critical step in gene regulation. While DCP2 is the catalytic subunit in the decapping complex, its activity is strongly enhanced by multiple factors, particularly DCP1, which is the major activator in yeast. However, the precise role of DCP1 in metazoans has yet to be fully elucidated. Moreover, in humans, the specific biological functions of the two DCP1 paralogs, DCP1a and DCP1b, remain largely unknown. To investigate the role of human DCP1, we generated cell lines that were deficient in DCP1a, DCP1b, or both to evaluate the importance of DCP1 in the decapping machinery. Our results highlight the importance of human DCP1 in decapping process and show that the EVH1 domain of DCP1 enhances the mRNA-binding affinity of DCP2. Transcriptome and metabolome analyses outline the distinct functions of DCP1a and DCP1b in human cells, regulating specific endogenous mRNA targets and biological processes. Overall, our findings provide insights into the molecular mechanism of human DCP1 in mRNA decapping and shed light on the distinct functions of its paralogs.
-
- Chromosomes and Gene Expression
- Computational and Systems Biology
Genes are often regulated by multiple enhancers. It is poorly understood how the individual enhancer activities are combined to control promoter activity. Anecdotal evidence has shown that enhancers can combine sub-additively, additively, synergistically, or redundantly. However, it is not clear which of these modes are more frequent in mammalian genomes. Here, we systematically tested how pairs of enhancers activate promoters using a three-way combinatorial reporter assay in mouse embryonic stem cells. By assaying about 69,000 enhancer-enhancer-promoter combinations we found that enhancer pairs generally combine near-additively. This behaviour was conserved across seven developmental promoters tested. Surprisingly, these promoters scale the enhancer signals in a non-linear manner that depends on promoter strength. A housekeeping promoter showed an overall different response to enhancer pairs, and a smaller dynamic range. Thus, our data indicate that enhancers mostly act additively, but promoters transform their collective effect non-linearly.