1. Developmental Biology

FBXO24 modulates mRNA alternative splicing and MIWI degradation and is required for normal sperm formation and male fertility

  1. Zhiming Li  Is a corresponding author
  2. Xingping Liu
  3. Yan Zhang
  4. Yuanyuan Li
  5. Liquan Zhou  Is a corresponding author
  6. Shuiqiao Yuan  Is a corresponding author
  1. Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, China
  2. Laboratory of Animal Center, Huazhong University of Science and Technology, China
https://doi.org/10.7554/eLife.91666.3
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  1. Zhiming Li
  2. Xingping Liu
  3. Yan Zhang
  4. Yuanyuan Li
  5. Liquan Zhou
  6. Shuiqiao Yuan
(2024)
FBXO24 modulates mRNA alternative splicing and MIWI degradation and is required for normal sperm formation and male fertility
eLife 12:RP91666.
https://doi.org/10.7554/eLife.91666.3
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Abstract

Spermiogenesis is a critical, post-meiotic phase of male gametogenesis, in which the proper gene expression is essential for sperm maturation. However, the underFlying molecular mechanism that controls mRNA expression in the round spermatids remains elusive. Here, we identify that FBXO24, an orphan F-box protein, is highly expressed in the testis of humans and mice and interacts with the splicing factors (SRSF2, SRSF3, and SRSF9) to modulate the gene alternative splicing in the round spermatids. Genetic mutation of FBXO24 in mice causes many abnormal splicing events in round spermatids, thus affecting a large number of critical genes related to sperm formation that were dysregulated. Further molecular and phenotypical analyses revealed that FBXO24 deficiency results in aberrant histone retention, incomplete axonemes, oversized chromatoid body, and abnormal mitochondrial coiling along sperm flagella, ultimately leading to male sterility. In addition, we discovered that FBXO24 interacts with MIWI and SCF subunits and mediates the degradation of MIWI via K48-linked polyubiquitination. Furthermore, we show that FBXO24 depletion could lead to aberrant piRNA production in testes, which suggests FBXO24 is required for normal piRNA counts. Collectively, these data demonstrate that FBXO24 is essential for sperm formation by regulating mRNA alternative splicing and MIWI degradation during spermiogenesis.

eLife assessment

This important study provides insights into the role of FBXO24 in controlling spermiogenesis and male fertility in mice. The mouse models used and the data are convincing. This paper will interest biomedical researchers working on reproductive biology and fertility control.

https://doi.org/10.7554/eLife.91666.3.sa0

Introduction

Spermatogenesis is a dynamic but well-organized process in which germ cells develop in the seminiferous tubules of the testis. Spermatogenesis comprises three phases: mitosis of the spermatogonia, meiosis of spermatocytes, and differentiation of round spermatids into mature sperm. The third phase is also known as spermiogenesis. During spermiogenesis, round spermatids undergo many significant changes, including the loss of cytoplasm, migration of cytoplasmic organelles, formation of the acrosome and flagellum, condensation of nuclei, and reorganization of mitochondria around the sperm midpiece (Jha et al., 2017). In mice, spermiogenesis is subdivided into 16 steps based on changes in acrosome structure and nuclear compaction (Ahmed and de Rooij, 2009; Meistrich and Hess, 2013). Coordinated regulation of gene expressions in the round spermatids is essential for sperm formation. However, the underlying molecular mechanism that controls mRNA expression during spermiogenesis remains elusive.

FBXO24 (F-Box Protein 24) is a member of the F-box protein family characterized by an F-box domain. The F-box proteins constitute one of the subunits of the ubiquitin protein ligase complex called SCF (SKP1-cullin-F-box). The interaction between FBXO24 and the SCF subunits is unknown. The F-box domain recruits the F-box protein to the SCF complex, and the carboxy-terminal domain is a putative protein–protein interaction domain. According to the different substrate recognition domains, F-box protein was generally divided into three subclasses: F-box/WD repeat-containing protein (FBXW), leucine-rich repeat protein (FBXL), and F-box only protein (FBXO) with/without other unknown domain (Kipreos and Pagano, 2000). Compared with the studies of FBXW and FBXL, the roles of most members of the FBXO subfamily have yet to be defined. FBXO24 belongs to the FBXO class. Previous studies demonstrated some FBXO proteins could regulate spermatogenesis. For example, FBXO47 has been reported to be essential for meiosis I progression in the spermatocytes of mouse testis (Hua et al., 2019; Tanno et al., 2022), and FBXO43 was associated with male infertility and teratozoospermia (Ma et al., 2019). In addition, FBXO24 has been reported to recognize deacetylated nucleoside diphosphate kinase A (NDPK-A) to enhance its degradation in HeLa cells (Chen et al., 2015). In H1299 cells, FBXO24 could promote cell proliferation by mediating ubiquitination and degradation of protein arginine methyltransferase 6 (PRMT6) (Chen et al., 2020). However, the molecular and biological functions of FBXO24 remain unclear, especially its role in male fertility and sperm formation.

In this study, we find that the transcripts encode FBXO24 are abundantly expressed in the testis of humans and mice. The loss function of FBXO24 in mice resulted in male sterility, with sperm possessing a collapsed mitochondrial sheath and uncompacted chromatin. We revealed that FBXO24 interacts with splicing factors (e.g., SRSF2, SRSF3, and SRSF9) to mediate gene expressions during spermiogenesis. Unexpectedly, we discovered that FBXO24 mediates MIWI ubiquitination and regulates the chromatoid body (CB) architecture and piRNA production. Together, our study demonstrated that FBXO24 plays a critical role in controlling gene expression in haploid spermatids and sperm formation during spermiogenesis.

Results

FBXO24 expressed in haploid spermatids during spermiogenesis

Interestingly, qPCR (Realtime quantitative PCR) analysis of multiple mouse organs showed that FBXO24 mRNA was predominantly expressed in mouse testes (Figure 1A). Multi-alignment and phylogenetic analysis revealed that Fbxo24 encodes a highly conserved protein expressed in mammals, including humans and mice (Figure 1—figure supplement 1A). FBXO24 has two domains, F-box and regulator of chromatin condensation 1 (RCC1), which were also highly conserved in humans and mice (Figure 1—figure supplement 1B). Consistent with this result, transcription expression of analysis of FBXO24 in various human organs from the GE-mini database revealed FBXO24 also a testis-enriched expression in human testes (Figure 1—figure supplement 1C). Further qPCR analysis of the testis at different developmental stages and purified testicular cells showed that FBXO24 mRNA was highly expressed in the round spermatids and elongating spermatids (Figure 1B, C). We used the CRISPR/Cas9-mediated genome-editing system to generate a mouse model containing the Fbxo24 gene with a C-terminal HA epitope tag (Liu et al., 2019). To examine the protein level of FBXO24 in testes, we then characterized the tissue expression pattern of FBXO24 in various organs from adult Fbxo24-HA-tagged transgenic mice (Figure 1—figure supplement 1D). Consistent with the mRNA expression showing a testis-enriched expression of FBXO24, FBXO24HA-Tag protein was exclusively expressed in the testes (Figure 1D), suggesting FBXO24 plays a role in sperm formation. To explore the subcellular localization of FBXO24 in male germ cells, we performed immunofluorescence analysis by co-staining of FBXO24HA-Tag protein with γ-H2AX (a marker of meiotic DNA damage response) or PNA (an acrosome marker) in adult Fbxo24-HA-Tagged transgenic mouse testes. The results showed that FBXO24HA-Tag protein was exclusively localized in the nuclei and cytoplasm of spermatids at stages VI–IX (Figure 1E, F), demonstrating FBXO24 expressed in the round and elongating spermatids at steps 6–9. These results indicate that FBXO24 is an evolutionarily conserved testis-enriched protein specifically expressed in haploid spermatids during spermiogenesis.

Figure 1 with 1 supplement see all
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Expression profiles of FBXO24 during testicular development and spermatogenesis in mice.

(A) qPCR analysis of FBXO24 mRNA levels in multiple organs in mice. n = 3/group. Data are mean ± standard deviation (SD). *p < 0.05. (B) qPCR analysis of FBXO24 mRNA levels in developing testes at postnatal day 0 (P0), P3, P7, P14, P28, and P35. n = 3/group. Data are mean ± SD. *p < 0.05. (C) qPCR analysis of FBXO24 mRNA levels in isolated spermatogenic cell populations, including spermatogonia (SG), Sertoli cells (SE), spermatocytes (SC), round spermatids (RS), and elongating spermatids (ES). n = 3/group. Data are mean ± SD. *p < 0.05. (D) Western blot analysis of FBXO24-HA expression in the tissues of adult transgenic mice. (E–F) Co-immunostaining of FBXO24-HA red) and (E) γH2AX (green) or (F) PNA (green) in adult Fbxo24-HA testes. Scale bars = 25 μm. DNA (blue) is stained by DAPI (4,'6-Diamidin-2-phenylindole dihydrochloride). Spermatogenic stages are noted.

Figure 1—source data 1

Raw western blot for Figure 1D.

https://cdn.elifesciences.org/articles/91666/elife-91666-fig1-data1-v1.zip

FBXO24 deficiency results in defective spermiogenesis and male infertility in mice

To reveal the role of FBXO24 in male germ cell development and sperm formation, we generated Fbxo24 knockout (KO) mice using CRISPR/Cas9 gene editing technology. Two small guide RNAs (sgRNAs) were designed to target exon 3, which encodes the conserved F-box domain. To validate nucleotide deletion at the targeted loci, the corresponding genomic regions of Fbxo24 were evaluated by PCR. qPCR analysis verified the mRNA expression of Fbxo24 was almost undetectable in Fbxo24 KO mouse testes (Figure 2A; Figure 2—figure supplement 1A,B). To test the fecundity of Fbxo24 KO mice, we crossed Fbxo24 KO mice with fertility-proved wild-type (WT) mice for at least 6 months. The fertility test results showed that Fbxo24 KO males were completely infertile, but the Fbxo24 KO females were fertile (Figure 2B). Interestingly, we observed the testis size and the ratio of testis weight to body weight were comparable between Fbxo24 KO males and controls (Figure 2C; Figure 2—figure supplement 1C). Consistent with these observations, histological analysis of adult testes and epididymis showed no obvious abnormalities in Fbxo24 KO mice compared with WT (Figure 2—figure supplement 1D). However, in Fbxo24 KO mice, we found fewer elongating spermatids (at stages IX–X) and condensing spermatids (at stage XII) and a reduced number of condensed spermatids (at stages II–VIII) (Figure 2D,E), concomitant with increased apoptotic signals detected in late spermatids (Figure 2—figure supplement 1E,F), which suggesting FBXO24 deficiency could affect spermatid development during spermiogenesis. To further demonstrate the critical function of FBXO24 during spermiogenesis, we attempted to use the Fbxo24-HA-tagged transgenic mice to rescue sperm formation and fertility in Fbxo24 KO males. As expected, we found that expression of the transgenic FBXO24 could rescue the defects in spermiogenesis and infertility observed in Fbxo24 KO male mice (Figure 2F; Figure 2—figure supplement 1G), which confirmed the specific targeting of Fbxo24 in the mutants and the true role of FBXO24 in spermiogenesis. Together, these data indicate that depletion of FBXO24 causes defective spermiogenesis, leading to male infertility.

Figure 2 with 1 supplement see all
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FBXO24 deletion impairs spermatogenic defects in the late steps of spermiogenesis.

(A) qPCR analysis indicates that FBXO24 mRNA is markedly decreased in the testis of Fbxo24 knockout (KO) as compared to wild-type (WT). n = 3 mice /group. ***p < 0.001. (B) The fertility tests of WT and Fbxo24 KO male mice mated with fertile female mice are shown. n = 3 mice /group. Error bars represent mean ± standard deviation (SD). ***p < 0.001; ns: not significant. (C) Histological images of testes and epididymis of WT and Fbxo24 KO mice at 8 weeks old are shown. (D) Periodic acid-Schiff (PAS)–hematoxylin staining of Fbxo24 KO testis at 8 weeks old contained less- condensed late spermatids (red arrows). Spermatogenic stages are noted. RS, round spermatids; ES, elongating spermatids; Pl, preleptotene; P, pachytene; Z, zygotene; D, diplotene. Scale bars = 25 μm. (E) The number of late spermatids is significantly reduced in Fbxo24 KO testis. Ratios of spermatids and Sertoli cells in tubule cross-sections of specific stages of seminiferous epithelial cycles and corresponding spermatid development steps are shown. n = 3 mice. Data are mean ± SD. *p < 0.05. (F) Litter sizes of mating tests. F1 generation of intercrosses between the indicated males and Fbxo24+/− females are shown. Each dot represents one litter.

Ablation of FBXO24 in mice causes disorganized mitochondrial sheath of spermatozoa

To elucidate the causes of sterility in Fbxo24 KO males, we examined sperm count, sperm motility, and sperm morphology in Fbxo24 KO mice. The results showed that the sperm count and motility were significantly reduced in Fbxo24 KO mice compared with WT (Figure 3A,B). Inspiringly, we found that most of the spermatozoa in Fbxo24 KO mice displayed a disorganized mitochondrial sheath and had abnormal bending of the flagella (Figure 3C). In WT spermatozoa, the midpiece connected smoothly to the principal piece; however, in Fbxo24 KO spermatozoa, a gap in the midpiece can be observed (Figure 3C,D), leading to many sperm with an abrupt bending of the tail in the region of the neck and midpiece (Figure 3—figure supplement 1A). In addition, we observed the midpiece length was significantly shorter in Fbxo24 KO mouse spermatozoa compared with WT (21.67 ± 1.524 μm in WT vs. 14.80 ± 3.430 μm in Fbxo24 KO) (Figure 3E). To better reveal spermatozoon abnormalities in Fbxo24 KO mice, we decided to examine the ultrastructure of the spermatozoa using a scanning electron microscope (SEM). In WT spermatozoa, the midpiece was normally populated with many mitochondria in a spiraling fashion. However, in Fbxo24 KO spermatozoa, mitochondria near the annulus were absent, resulting in a shorter mitochondrial sheath (Figure 3F). Transmission electron microscope (TEM) further confirmed that mitochondria were not present near the annulus or neck and left apparent gaps along the mitochondrial sheath in Fbxo24 KO spermatozoa (Figure 3G), indicating mitochondrial sheath formation was disrupted. In addition, some mitochondria in the midpiece of Fbxo24 KO spermatozoa appeared large and vacuolar. To determine whether the failure in the assembly of the mitochondrial sheath could cause disrupted flagellar development, we examined cross-sections of the Fbxo24 KO sperm flagellum. In the midpiece, well-defined outer dense fibers (ODFs) and the axoneme consisting of the typical ‘9 + 2’ microtubules were observed in WT spermatozoa, whereas the Fbxo24 KO spermatozoa displayed incomplete axonemes with missing axonemal microtubules (Figure 3H). In the principal piece, the Fbxo24 KO spermatozoa also showed completely or partially lacking ODFs and a typical ‘9 + 2’ microtubular structure (Figure 3H). These severe flagellar defects may explain why Fbxo24 KO spermatozoa displayed decreased motility.

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Sperm mitochondria and flagella are defective in FBXO24-deficient mice.

Quantification of sperm counts (A) and sperm motility (B) from wild-type (WT) and Fbxo24 knockout (KO) epididymis are shown. n = 3/group. Error bars represent mean ± standard deviation (SD). **p < 0.01. ***p < 0.001. (C) Sperm morphological images show the defective sperm of Fbxo24 KO mice. Red arrows indicate abnormal gaps in the mitochondrial sheath. Scale bars = 5 μm. (D) Immunofluorescence images of sperm from WT and Fbxo24 KO epididymis. PNA (acrosome, green), MitoTracker (mitochondria, red), and DAPI (nucleus, blue). White arrows indicate the weak or absent staining of MitoTracker. Scale bars = 5 μm. (E) Quantifications of the length of sperm midpiece from WT and Fbxo24 KO mice are shown. n = 100/group. Error bars represent mean ± SD. ***p < 0.001. (F) Scanning electronic microscopy (SEM) images indicate the mitochondria detachment from the flagellum of Fbxo24 KO sperm. Right panel insets show higher magnification of sperm midpiece. The arrowheads indicate the annulus. The dashed red line indicates a region where mitochondria are absent. Scale bars = 2 μm. (G) Transmission electronic microscopy (TEM) images indicate the mitochondria defects in three regions of Fbxo24 KO sperm midpiece in the longitudinal sections. Nu, nucleus. The dashed red lines indicate a region where mitochondria are absent. The white arrowhead indicates sperm annulus. Scale bar = 0.2 μm. (H) TEM images indicate the ultrastructure of the midpiece, principal piece and end piece of WT and Fbxo24 KO sperm flagellum in the cross-sections. Arrows indicate the mitochondria (MI), outer dense fiber (ODF) and axoneme. Scale bar = 0.2 μm. Western blot shows the levels of proteins of mitochondria (I) and axoneme (J) of WT and Fbxo24 KO sperm. GAPDH serves as a loading control.

Figure 3—source data 1

Raw western blot for Figure 3I, J.

https://cdn.elifesciences.org/articles/91666/elife-91666-fig3-data1-v1.zip

We next asked whether FBXO24 deletion influences the protein levels of mitochondrial and flagellum components in spermatozoa through Western blot analysis of spermatozoa. We found that the levels of MFN2 (a marker of mitochondrial fusion) and DRP1 (a marker of mitochondrial fission) were decreased in Fbxo24 KO spermatozoa (Figure 3I; Figure 3—figure supplement 1B). Moreover, the protein expression of ODF2, AKAP3, and TSSK4, which were directly related to the formation of the sperm tail (Wang et al., 2015; Donkor et al., 2004; Xu et al., 2020), were all decreased in Fbxo24 KO spermatozoa (Figure 3J; Figure 3—figure supplement 1B). This was supported by immunofluorescence analysis of ODF2 and AKAP3 with a reduced signals in Fbxo24 KO flagella (Figure 3—figure supplement 1C,D). Interestingly, we found that the level of the CUL1, a scaffold for E3 ubiquitin ligase, was also significantly reduced in Fbxo24 KO spermatozoa (Figure 3J). Together, these results indicate that FBXO24 is critical for mitochondrial sheath formation and could modulate the protein expression levels of mitochondrial and flagellar components to maintain sperm motility.

The mitochondrial and CB architecture were affected in FBXO24-deficient round spermatids

To further explore whether the mitochondrial organelle morphology in round spermatids was affected upon FBXO24 depletion, we examined the ultrastructure of WT and Fbxo24 KO testes by TEM. Notably, the mitochondria in the round spermatids of Fbxo24 KO testis displayed vacuolar and had abnormal central cristae (Figure 4A,B). Interestingly, we identified that the CB was structurally maintained in Fbxo24 KO round spermatids, whereas the size was often larger than WT (Figure 4C,D). CB was considered to have the functions in the assembly of the mitochondria during the spermiogenesis, and dysfunctional transformation of the CB in mouse spermatids could cause spermatozoa to possess a collapsed mitochondrial sheath (Shang et al., 2010), which resembled the phenotype observed in our Fbxo24 KO mice. To explore the molecular reason for the abnormal CB formation, we examined the protein expression levels of CB components in WT and Fbxo24 KO testes. Since MIWI was a core component of CB and also identified as an FBOX24 interacting partner from our immunoprecipitation-mass spectrometry (IP-MS) (Supplementary file 1), we focused on the examination of MIWI expression between WT and Fbxo24 KO testes. Western blot analyses revealed that the protein level of MIWI was remarkably increased in the Fbxo24 KO testes compared with WT (Figure 4E). However, the level of other proteins of CB, and PRMT6 (reported to be the substrate of FBXO24) (Chen et al., 2020) appeared to be unchanged in Fbxo24 KO testes compared with WT testis (Figure 4E). These results suggest that FBXO24 is essential for maintaining the normal architecture of mitochondria and CB in the round spermatids.

Figure 4
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Ablation of FBXO24 affects mitochondria and chromatoid body (CB) architecture in the round spermatids.

(A) Transmission electron microscope (TEM) images show vacuolar mitochondria with disorganized cristae in the round spermatids of Fbxo24 knockout (KO) testes. Right panel insets show higher magnification of mitochondria. Scale bars = 1 μm. (B) Quantification of the number of vacuolar mitochondria. Error bars represent mean ± SD. n = 3. ***p < 0.001. (C) TEM images showing decondensed and enlarged CB with an irregular network in the round spermatids of Fbxo24 KO testes. Right panel insets show a higher magnification of CB. Scale bars = 1 μm. (D) Quantification of size/diameters of CB. Error bars represent mean ± SD. n = 3. *p < 0.05. (E) Western blot analysis expression levels of CB components and PRMT6 in testes from wild-type (WT) and Fbxo24 KO mice at 8 weeks old. GAPDH serves as a loading control.

Figure 4—source data 1

Raw western blot for Figure 4E.

https://cdn.elifesciences.org/articles/91666/elife-91666-fig4-data1-v1.zip

Histones failed to be replaced in FBXO24-deficient mouse spermatozoa

To investigate the nuclear morphology of spermatozoa, we examined the sperm head structures of WT and Fbxo24 KO spermatozoa by SEM. The Fbxo24 KO spermatozoa exhibited abnormal head morphologies that deviated from the flat, crescent-shaped structures of their WT counterparts (Figure 5A). Specifically, the defects of Fbxo24 KO spermatozoa included a disorganized anterior acrosome (AS) and the absence of a distinct equatorial segment (EQ), post-acrosomal sheath (PAS), ventral spur (VS), and sharp hook rim (HR) (Figure 5A). TEM analysis further revealed less-condensed nuclei of Fbxo24 KO spermatozoa (Figure 5B), suggesting an abnormal nuclear chromatin compaction was affected in Fbxo24 KO sperm head. Fbxo24 KO spermatozoa exhibited elevated levels of DNA damage by TUNEL analysis (Figure 5—figure supplement 1A). In many KO mice studies, impaired chromatin condensation is frequently associated with abnormal sperm head morphology (Okada, 2022). We then investigated the potential causes of abnormal head structures in Fbxo24 KO spermatozoa. Given that impaired histone-to-protamine exchange can result in less-condensed nuclei in spermatozoa, we examined histone and protamine levels in Fbxo24 KO mouse sperm. Western blot revealed increased levels of all core histones H2A, H2B, H3, H4, and transition protein TNP1 but reduced levels of PRM2 in Fbxo24 KO sperm (Figure 5C,D). We found that FBXO24 did not have the interactions with histones H2A, H2B, H3, H4, and transition protein TNP1 (Figure 5—figure supplement 1B). Moreover, we found a reduced protein level of PHF7, TSSK6, and RNF8 in Fbxo24 KO testis (Figure 5E,F), which have been reported to regulate the chromatin structure to facilitate histone-to-protamine replacement (Jha et al., 2017; Lu et al., 2010; Wang et al., 2019). These data suggest that FBXO24 deletion causes an incomplete histone-to-protamine exchange and defective chromatin compaction during spermiogenesis.

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FBXO24 deficiency in mice impairs sperm histone-to-protamine exchange.

(A) Scanning electron microscope (SEM) images show the abnormality of Fbxo24 knockout (KO) sperm head. AA, anterior acrosome; EQ, equatorial segment; PAS, post-acrosomal segment; VS, ventral spur; HR, hook rim. Scale bars = 2 μm. (B) Transmission electron microscopy (TEM) images show the decondensed nucleus (Nu) of Fbxo24 KO sperm. Scale bars = 1 μm. (C) Western blot analysis of the expression of histones (H2A, H2B, H3, and H4), transition proteins (TNP1), and protamines (PRM2) from wild-type (WT) and Fbxo24 KO sperm are shown. GAPDH serves as a loading control. (D) Quantification of protein levels. Error bars represent mean ± standard deviation (SD), n = 3. *p < 0.05. (E) Western blot analysis of the expression of indicated proteins in WT and Fbxo24 KO testis. GAPDH serves as a loading control. (F) Quantification of protein levels. Error bars represent mean ± SD, n = 3. *p < 0.05.

Figure 5—source data 1

Raw western blot for Figure 5C, E.

https://cdn.elifesciences.org/articles/91666/elife-91666-fig5-data1-v1.zip

FBXO24 depletion leads to aberrant expression of spermiogenic genes in the round spermatids

To further investigate the underlying molecular changes in sperm formation upon FBXO24 depletion, we performed RNA-seq using purified round spermatids (RS) from adult Fbxo24 KO and WT males (Figure 6—figure supplement 1A). RNA-seq analysis showed a large number of genes significantly differentially expressed in Fbxo24 KO round spermatids, including 5368 up-regulated and 4097 down-regulated genes (Figure 6A and Supplementary file 2). The Gene Ontology (GO) analysis revealed that the down-regulated genes were related to mitochondrion localization, chromatin organization, and protein K48-linked ubiquitination (Figure 6B and Supplementary file 3). The expression of many critical genes involved in mitochondrion localization (e.g., Hif1a, Nefl, Mgarp, Zmynd12, Map7d1, Bbs7, Mfn1, and Ube2j2) and chromatin organization (e.g., Rnf20, Ep300, Sirt1, Hmgb2, Hsf1, Cul4b, Kat5, and Phf7) were significantly decreased in Fbxo24 KO round spermatids (Figure 6C,D). Interestingly, we found that Sept4 and Sept12, the members of Septin family known to generate annuli of spermatozoa, were also down-regulated in Fbxo24 KO round spermatids (Figure 6—figure supplement 1B). The most up-regulated genes in Fbxo24 KO round spermatids were mainly related to cellular metabolic processes and organelle organization (Figure 6—figure supplement 1C). Furthermore, we identified an overlap between the down-regulated genes and the altered alternative splicing (AS) genes in Fbxo24 KO round spermatids (Figure 6E). A large number of mRNA splicing events were identified in Fbxo24 KO round spermatids (Figure 6F), including skipped exons (SE), alternative 5′ splice sites (A5SS), alternative 3′ splice sites (A3SS), mutually exclusive exons (MXE), and retained introns (RI). Interestingly, GO analysis of the abnormally spliced genes highlighted the functional categories involved in sperm formation, such as outer dynein arm assembly, mitochondrion localization, and chromatin organization (Figure 6—figure supplement 1D). Thus, the RNA-seq data revealed genetic ablation of Fbxo24 has a significant impact on the transcriptome of round spermatids, indicating that FBXO24 is required for gene expression in round spermatids during spermiogenesis.

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RNA-seq analyses of the round spermatids from FBXO24-deficient testes.

(A) Volcano plot of differentially expressed transcripts in the round spermatids (RS) of Fbxo24 knockout (KO) vs. wild-type (WT) mice. Each red (up-regulation) or blue (down-regulation) dot represents a significantly changed gene. (B) Gene Ontology (GO) term enrichment analysis of down-regulated transcripts of Fbxo24 KO RS. Gene expressions of mitochondrion localization (C) and chromatin organization (D) in RNA-seq analysis. (E) Venn diagrams showing the overlap between down-regulated genes and abnormal alternative splicing genes in Fbxo24 KO RS. (F) Summary of differential splicing evens in Fbxo24 KO RS. The number of each category of alternative splicing is indicated.

Loss of FBXO24 results in aberrant mRNA alternative splicing in the round spermatids

Since FBXO24 expressed in the nucleus of the round spermatids, we speculated that FBXO24 might interact with the key splicing factors. To test this hypothesis, we performed IP-MS using the HA antibody to unbiasedly identify the interactome of FBXO24 in testes. The IP-MS results showed many splicing factors, such as SRSF2, SRSF3, and SRSF9, highly enriched in HA-immunoprecipitates (Supplementary file 1). Through co-immunoprecipitation assays, we confirmed FBXO24 has indeed interacted with splicing factors SRSF2, SRSF3, and SRSF9 in the testes (Figure 7A). Interestingly, we found a significant decrease in SRSF2, SRSF3, and SRSF9 protein expression levels in Fbxo24 KO testes compared to controls (Figure 7B,C). Because many alternative splicing genes related to sperm formation were affected in Fbxo24 KO round spermatids by RNA-seq analysis, we asked if the splicing levels of corresponding exons of those genes were altered. To this end, we performed RT-PCR on purified round spermatids to verify the alternative splicing alteration. The RT-PCR results showed that many genes related to sperm formation, including mitochondria (Mfn1), flagellum (Zmynd12, Map7d1, and Bbs7), chromatin (Phf7 and Kat5), acrosome (Nucb2), appeared to be significantly alternative splicing changes in their corresponding exons (Figure 7D–G). In addition, we found that the alternative splicing of Ube2j2, a ubiquitin-conjugating enzyme, was also affected in Fbxo24 KO round spermatids (Figure 7H). Of note, sperm motility of Ube2j1 KO mice was also severely impaired (Koenig et al., 2014). Together, these results indicate that FBXO24 could interplay with key splicing factors to regulate the mRNA splicing of some essential genes related to sperm formation.

Figure 7
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Aberrant alternative splicing of spermiogenesis genes in the round spermatids of FBXO24-deficient mice.

(A) Co-immunoprecipitation analysis of FBXO24 and the splicing regulators (SRSF2, SRSF3, and SRSF9) in Fbxo24-HA-tagged mice testis. Wild-type (WT) testis was used as a negative control. (B) Western blotting analysis of the splicing regulators in WT and Fbxo24 knockout (KO) testis. GAPDH was used as a loading control. (C) Quantification of protein levels. Error bars represent mean ± standard deviation (SD), n = 3. *p < 0.05. Validation of abnormal alternative splicing genes related to (D) mitochondria (Mfn1), (E) flagellum (Zmynd12, Map7d1, and Bbs7), (F) chromatin (Phf7 and Kat5), (G) acrosome (Nucb2), and (H) ubiquitination (Ube2j2). The top panels represent RT-PCR analysis of indicated genes in WT and Fbxo24 RS. Gapdh serves as a loading control. The middle panels show the quantification of percent spliced in (PSI) and alternative sites in RNA-seq. Error bars represent mean ± SD, n = 2. *p < 0.05. The bottom panels represent the schematic diagram of alternative sites exons.

Figure 7—source data 1

Raw western blot for Figure 7A, B.

https://cdn.elifesciences.org/articles/91666/elife-91666-fig7-data1-v1.zip
Figure 7—source data 2

Raw RT-PCR gel for Figure 7D–H.

https://cdn.elifesciences.org/articles/91666/elife-91666-fig7-data2-v1.zip

FBXO24 mediates K48-linked polyubiquitination of MIWI

Since the increased expression of MIWI was found in the testes of Fbxo24 KO mice, we sought to determine whether FBXO24 interacts with MIWI and affects its degradation via ubiquitination. We ectopically expressed FBXO24-mCherry in HEK293T cells and incubated FBXO24-mCherry with the testicular protein lysate. MIWI and CUL1 were detected in FBXO24 immunoprecipitates (Figure 8A), suggesting that FBXO24 could bind MIWI and CUL1 in vivo. To confirm that FBXO24 endogenously interacted with its candidates, we performed immunoprecipitation (IP) with the testis of Fbxo24-HA-tagged transgenic mice. Through co-immunoprecipitation assays, we identified that FBXO24 indeed interacts with MIWI and SCF subunits, including CUL1, SKP1, and RBX1. (Figure 8B). To further dissect the binding abilities between FBXO24 and MIWI, we examined which domain was responsible for the interaction through the truncated proteins of FBXO24, which only contain the F-box or RCC1 domain (Figure 8C). The IP results showed that both the F-box and RCC1 domains of FBXO24 could bind with MIWI (Figure 8D). Furthermore, we found that FBXO24 leads to a decreased level of MIWI in a concentration-dependent manner, suggesting FBXO24 modulates the degradation of MIWI (Figure 8E). Because K48- and/or K63-linked polyubiquitination was reported to be responsible for degradation, trafficking, and phosphatase activation (Tracz and Bialek, 2021), we then asked which kind of modification participates in MIWI ubiquitination. We found that overexpression of FBXO24 enhanced polyubiquitination of MIWI in the presence of Ub (K48) but did not enhance polyubiquitination of MIWI in the presence of Ub (K63) (Figure 8F). Interestingly, we also found that FBXO24 contributes to the decreased expression of MIWI in a ubiquitin-dependent manner, and the ubiquitin level of MIWI appeared to be reduced in FBXO24 KO round spermatids (Figure 8G,H). These data suggest that FBXO24 interacts with SCF subunits and mediates the degradation of MIWI via K48-linked polyubiquitination.

Figure 8
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FBXO24 interacts with MIWI and mediates its K48-linked polyubiquitination.

(A) HEK293T cells transfected with empty FBXO24-mCherry or mCherry vector. Anti-mCherry beads were used for immunoprecipitation (IP), and western blots were used to detect the CUL1 (left panel) and MIWI (right panel) expression. (B) The testis lysate of Fbxo24-HA-tagged mice were immunoprecipitated with anti-HA beads. Western blots were used to detect the HA, CUL1, MIWI, SKP1, and RBX1expression. (C) Schematic structures of the truncated FBXO24 protein are shown. Broken boxes show the domain of F-box and regulator of chromosome condensation 1 (RCC1). (D) HEK293T cells were transfected with indicated plasmids. IP was performed using the anti-mCherry antibody. (E) Western blot analysis of HEK293T cells transfected with indicated FBXO24-mCherry and 2 μg MIWI-myc plasmids. The cell lysates were immunoblotted with anti-mCherry and anti-myc antibodies. (F) FBXO24 mediated the ubiquitination of MIWI in the presence of Ub (K48) not Ub (K63). (G) HEK293T cells were transfected with indicated FBXO24-mCherry, 2 μg MIWI-myc, and 2 μg Ub-HA plasmids. The cell lysates were immunoblotted with the anti-mCherry, anti-myc, and anti-HA antibodies. GAPDH serves as a loading control. (H) Ubiquitination analysis of MIWI in the round spermatids of Fbxo24 knockout (KO) mice. The cells were treated with MG132 (10 µM) in the ubiquitination assay.

Figure 8—source data 1

Raw western blot for Figure 8A, B, D–H.

https://cdn.elifesciences.org/articles/91666/elife-91666-fig8-data1-v1.zip

FBXO24 is required for normal piRNA production in the testes

Because piRNAs could be loaded onto MIWI (Kawase et al., 2022), we next examined whether the population of piRNAs was affected in Fbxo24 KO testes. Sequencing of small RNA libraries constructed from total RNA revealed that the expression of miRNAs was not extensively changed in Fbxo24 KO testes, with only 20 up- and 13 down-regulated miRNAs (Figure 9A; Figure 9—figure supplement 1A, and Supplementary file 4). However, a large number of piRNAs were identified to be differentially expressed in Fbxo24 KO testes, 463 up- and 128 down-regulated piRNAs (Figure 9—figure supplement 1B and Supplementary file 5). After normalized with miRNA counts, the relative amount of total piRNAs was also increased in Fbxo24 KO testes (Figure 9—figure supplement 1C). Specifically, an increased piRNAs profile was observed in the genomic regions between 2 kb upstream of transcriptional start sites (TSS) and 2 kb downstream of transcriptional end sites (TES) in Fbxo24 KO testes (Figure 9B). To assess the potential functions of the association of piRNAs to different functional gene regions, we analyzed the piRNAs mapping density of varying gene regions, including coding region (CDS), 5′ and 3′ untranslated region (UTR), and intron, as well as transposable element (TE), including retrotransposon (LTR, LINE, and SINE) and DNA transposon. The results showed that the intron- and retrotransposon-derived piRNAs were the most affected types in Fbxo24 KO testes (Figure 9C and Supplementary file 6). Furthermore, we analyzed the abundance and size of piRNA populations in adult Fbxo24 KO and WT testes. The results showed that the number of 29–31 nt piRNAs were remarkably increased in Fbxo24 KO testes compared with WT (Figure 9D). In addition, we found many up-regulated piRNAs in Fbxo24 KO testes were also presented in previously published data of MIWI immunoprecipitates (Reuter et al., 2011; Figure 9E), suggesting that the increased piRNAs were MIWI-bound piRNAs. The first nucleotide and 10th nucleotide of repeat piRNAs of Fbxo24 KO testes exhibited a similar strong bias compared with piRNAs of WT testes, indicating the amplification process of the ping-pong cycle was not affected (Figure 9—figure supplement 1D). Together, these results show that the loss function of FBXO24 results in aberrant piRNA production in testes, suggesting FBXO24 is related to normal piRNA counts of the testis.

Figure 9 with 1 supplement see all
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Small RNA-seq analysis of testes from FBXO24-deficient mice.

(A) A scatter plot of differentially expressed miRNA is shown. Red and green dots represent up- and down-regulated miRNA (fold change >2, p < 0.05), respectively. (B) Genomic distribution of piRNA profile in Fbxo24 knockout (KO) vs. wild-type (WT) testis. piRNA levels were examined in each 200-bp interval of a 2-kb region up- and downstream of the annotated genes. (C) Scatter plots of differentially expressed piRNA mapping density (reads/kb) of the coding region (CDS), 5′ and 3′ untranslated region (UTR), and intron, as well as transposable element (TE), including retrotransposon (LTR, LINE, and SINE) and DNA transposon. The piRNA read counts were normalized with miRNA. Red and green dots represent up- and down-regulated piRNA (fold change >2, p < 0.05), respectively. (D) The size distribution of piRNAs in Fbxo24 KO vs. WT testis. (E) The top 10 up-regulated piRNAs in Fbxo24 KO testis exist in MIWI immunoprecipitates of GSM822760 data.

Discussion

In this study, we identified FBXO24 as a testis-enriched F-box protein conserved in mammals and highly expressed in the round spermatids and elongating spermatids. To define its biological roles in vivo, we generated genetically engineered Fbxo24 KO and Fbxo24 HA-tagged transgenic mouse models. Our functional study demonstrated that FBXO24 is essential for spermiogenesis and piRNA production. FBXO24 deficiency leads to remarkable histone retention and the disruption of histone-to-protamine transition in the mature sperm. A growing number of studies have demonstrated impaired chromatin condensation can cause nuclear damages as DNA denaturation or fragmentation often associated with male infertility (Filatov et al., 1999). The molecular basis of nuclear condensation is related to key events such as the expression of testis-specific histone variants, post-translational modifications of histones, and transient DNA strand breaks. Indeed, we found that, in the current study, FBXO24 could modulate the homeostasis of the histone-to-protamine transition regulators PHF7 (Wang et al., 2019), TSSK6 (Jha et al., 2017), and RNF8 (Lu et al., 2010), while RNF8 was reported to be independent to the process (Abe et al., 2021).

Although the abnormalities of mitochondrial sheaths are observed in infertile men (Kubo-Irie et al., 2005), the key proteins implicated in sperm mitochondrial sheath formation largely remain unclear due to a lack of good animal models with the typical phenotype. Notably, we found that the round spermatids and spermatozoon of Fbxo24 KO mice have impaired mitochondrial architecture, with mitochondrial size heterogeneity and a more open structure of the mitochondria with less crista. The defective mitochondria were associated with energetic disturbances and reduced sperm motility. Using MitoTracker staining and an electron microscope, we demonstrated the mitochondrial structure is severely disrupted in Fbxo24 KO spermatozoa. Previous studies showed abnormalities of the annulus (Sept4 and Sept12) (Kissel et al., 2005; Shen et al., 2017), and chromatid bodies (Tssk1 and Tssk2) (Shang et al., 2010; Dirami et al., 2015) can disrupt abnormal mitochondrial coiling. The CB was considered to migrate to the caudal end of the developing middle piece of the flagellum, moving in front of the mitochondria that are engaged in mitochondrial sheath morphogenesis (Fawcett et al., 1970). This study revealed that FBXO24 regulates spatiotemporal mitochondrial dynamics during spermiogenesis. FBXO24 might serve as a critical protein in regulating sperm mitochondrial sheath formation, which expands the knowledge of sperm mitochondria formation. In addition, we found that FBXO24 is indispensable for proper flagellum formation, such as axoneme and ODFs. Therefore, the reduction of sperm motility of Fbxo24 KO male mice observed in this study might attribute to the uncompleted mitochondrial formation of the middle piece and defective flagellum assembling.

It is worth mentioning that the RNA-seq data of the round spermatids showed that FBXO24 ablation leads to dysregulation in the mRNA expression of many genes related to chromatin, mitochondria, and flagellum. We found mRNA expression changes were related to the mRNA alternative splicing in Fbxo24 KO round spermatids. Combined with our IP evidence of molecular interactions between FBXO24 and the key splice factors (SRSF2, SRSF3, and SRSF9), it is reasonable to infer that dysregulation of these splicing factors inevitably would lead to more splicing errors in their target genes, thus amplifying the initial adverse effects and generating a vicious circle of aberrant splicing. Of note, many genetic changes identified in this study have been closely associated with sperm formation. For example, Zmynd12 (Dacheux et al., 2023), Bbs7 (Zhang et al., 2013), and Ube2j2 (Koenig et al., 2014) were required for flagellum function and male fertility. Map7d1 facilitates microtubule stabilization (Kikuchi et al., 2022) and Mfn1 deficiency leads to defects in mitochondrial activity and male infertility (Zhang et al., 2016). Phf7 modulates BRDT stability and histone-to-protamine exchange during spermiogenesis (Kim et al., 2020). Kat5 encodes an essential lysine acetyltransferase, which is involved in regulating gene expression and chromatin remodeling (Gehlen-Breitbach et al., 2023). Nucb2 suppresses the acrosome reaction in sperm within the mouse epididymis (Kim et al., 2023). Therefore, our study, for the first time, provides evidence that FBXO24 interacts with the splicing factors to regulate the alternative splicing of mRNA involved in round spermatid development. However, the exact regulatory mechanism of how FBXO24 facilitates the mRNA alternative splicing needs further study.

MIWI and piRNAs are highly abundant in round spermatids, but their levels start to decline normally in elongating spermatids and are completely eliminated in matured sperm (Deng and Lin, 2002). Interestingly, Mo-Fang Liu et al. reported that piRNA-triggered MIWI degradation occurs in late spermatids through the APC/C-26S proteasome pathway in vitro, which in turn leads to piRNA elimination (Zhao et al., 2013). As many piRNAs also appear to have the capacity to target diverse mRNA, they demonstrated that piRNAs act extensively as siRNAs to degrade specific mRNA (Zuo et al., 2016; Gou et al., 2014). Overexpression of a piRNA cluster in the mouse genome can reduce the expression of mRNA required for spermatogenesis, leading to male infertility (Goh et al., 2015). The level of 29–31 nt piRNAs was remarkably increased in Fbxo24 KO testes, which raises a possibility that piRNA-related transcriptional repression might contribute to the reduction of mature sperm in Fbxo24 KO mice. In the current study, we identified that testis-enriched FBXO24 could interact with MIWI and mediate its degradation via the K48-linked ubiquitination, which provides new insight into MIWI degradation during spermiogenesis.

In summary, this study revealed a novel role for FBXO24 in controlling mRNA alternative splicing of round spermatids and MIWI/piRNA pathway, and demonstrated that FBXO24 is required for mitochondrial organization and chromatin condensation in mouse spermatozoa (Figure 10). The results disclosed FBXO24 interacts with splicing factors to regulate the mRNA splicing of some essential genes required for spermiogenesis, which probably functionally connects the dysregulation of genes involved in mitochondria migration and histone removal during spermiogenesis. FBXO24 interplays with the subunits of the SCF complex and mediated MIWI degradation. This study extensively expands our understanding of the mechanistic explanation of FBXO24 for the regulation of spermiogenesis.

Figure 10
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A schematic model shows the FBXO24-mediated post-transcriptional regulation during spermiogenesis.

Fbxo24 interacts with key splicing factors (SRSF2, SRSF3, and SRSF9) to coordinate proper alternative splicing of the target mRNA transcripts involved in spermiogenesis. FBXO24 regulates the architectures of mitochondria and chromatid body through MIWI/piRNA pathway in the round spermatids.

Materials and methods

Ethics statement

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All the animal procedures were approved by the Institutional Animal Care and Use Committee protocols (#S2795) of Tongji Medical College, Huazhong University of Science and Technology, and the mice were housed in the specific pathogen-free facility of Huazhong University of Science and Technology. All experiments with mice were conducted ethically according to the Guide for the Care and Use of Laboratory Animal guidelines.

Generation of Fbxo24 mutant mice

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Fbxo24 KO mice were generated by CRISPR/Cas9 technology. Briefly, the two pairs of single-guided RNAs (sgRNAs) with the sequence sgRNA-1: 5′-TGTGGAGGCGCATCTGTCGAAGG-3′ and sgRNA-2 5′-GTCAAAGACTTGGTCGCCCTAGG-3′ were designed for targeting for the third exon of Fbxo24 gene. The sgRNAs were injected into the pronuclei of fertilized eggs, and the two-cell stage embryos were transferred into the oviducts of pseudopregnant C57BL/6J females in the next day to generate Fbxo24 mutant founder mice (F0). The F1 Fbxo24 heterozygous mice were produced by crossing the F0 founder mice with WT mice and further intercrossed F1 to obtain Fbxo24 KO mice carrying an 82-bp deletion in exon 3 of the Fbxo24 gene. A 197-bp band as the WT allele and a 115-bp band as the deleted allele were designed for genotyping by PCR amplification. The primers used are listed in Supplementary file 7.

Generation of Fbxo24-HA-tagged transgenic mice

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Fbxo24-HA-tagged transgenic mice were also generated by CRISPR/Cas9 technology and haploid embryonic stem cell microinjection. The sgRNAs of the C-terminus of the target gene Fbxo24 (AAGAAGAGGGGTTCAGCTCT) were synthesized, annealed, and ligated to the pX330-mCherry plasmid. For the construction of the HA-tag DNA donor, the sequences encoding the left homologous arm, the HA tag, and the right homologous arm were amplified and ligated to the linear pMD19T. DKO-AG-haESCs were transfected with CRISPR-Cas9 plasmid and HA tag DNA donor. At 24 hr after transfection, the mCherry-positive haploid cells were enriched. In 7–8 days after plating, single colonies were picked up, and positive colonies for the HA tag were selected by genomic DNA PCR amplification for Sanger sequencing. DKO-AG-haESCs with Fbxo24-C-HA were arrested in M-phase by culturing in a medium containing 0.05 μg/ml demecolcine for 10 hr and then used for intracytoplasmic injection as described previously (Zhong et al., 2015). Intracytoplasmic AG-haESC injection (ICAHCI) embryos were cultured in KSOM medium for 24 hr to reach the two-cell stage. 15–20 two-cell embryos were transferred into each oviduct of pseudo-pregnant females to produce Fbxo24-C-HA semi-cloned mice (F0). Then, the F0 female mice were crossed with WT male mice to generate heterozygous Fbxo24-C-HA F1 male mice for the experiments.

Antibodies

The details of all commercial antibodies used in this study are presented in Supplementary file 8.

Electron microscopy

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For TEM, samples were fixed in 4% paraformaldehyde (PFA) containing 0.05% glutaraldehyde in 0.1 M phosphate-buffered saline (PBS), then post-fixed in 1% osmium tetroxide. Dehydration was carried out in ethanol (25, 50, 75, and 95%), and the samples were embedded in Epon 812. Ultrathin sections with 80 nm thickness were prepared by ultramicrotome (Leica UC7, Leica Biosystems, Germany). Ultrathin sections were counterstained with uranyl acetate and lead citrate and examined with a TEM (Hitachi HT7700, Japan). For SEM, the samples were fixed in 2.5% glutaraldehyde solution in 0.1 M PBS and collected on poly-L-lysine-coated glass coverslips, followed by post-fixed in osmium tetroxide and dehydrated in graded ethanol series. Then, the samples were subjected to critical point drying and coated with gold/palladium for observation with an SEM (AZteclive Ultim Max 100, UK).

Sperm and histological analysis

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The epididymis isolated from 8-week-old mice and sperm allowed to swim into M16 medium (Sigma-Aldrich), which was maintained at 37°C and 5% CO2. After 30 min, the medium was collected and diluted for sperm number and motility assessment. To determine sperm percent motility, a 10-µl sample was loaded onto a clean slide glass and covered with a coverslip. Motility was graded according to the WHO criteria under positive phase contrast microscopy at a total magnification ×400 (Komori et al., 2006). The length of midpiece was measured from the sperm neck to the sperm annulus by MitoTracker staining. All results were obtained from experiments performed on at least 6 mice per genotype group and 100 sperm per mouse. Testes and epididymis from WT and KO mice were fixed in Bouin’s fixative and embedded in paraffin. For the histological analysis, sections of 5 μm were cut and stained with periodic acid-Schiff (PAS) or hematoxylin and eosin (H&E) after dewaxing and rehydration.

Immunofluorescence

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Testes were fixed in 4% PFA in PBS overnight at 4°C and then were sequentially soaked in 5, 10, 12.5, 15, and 20% sucrose in PBS and embedded in Tissue-Tek O.C.T. compound (Sakura 4583, Sakura Finetek USA, Inc, Torrance, CA) on dry ice. 5 μm cryosections were cut and washed with PBS three times (10 min per wash), then the cryo-sections were microwaved in 0.01 M sodium citrate buffer (pH 6.0) and cooled down to room temperature (RT) for antigen retrieval. After washing with PBS three times, the sections were blocked in a blocking solution (containing 3% normal donkey serum and 3% fetal bovine serum in 1% bovine serum albumin) for 1 hr. Then, the sections were incubated with primary antibodies overnight at 4°C and then incubated with secondary antibodies for 1 hr at RT. After washing with PBS and stained with DAPI, the sections were photographed under FluoView 1000 microscope (Olympus, Japan) with a digital camera (MSX2, Micro-shot Technology Limited, China). Images were merged using Adobe Photoshop (Adobe Systems, San Jose, CA).

TUNEL staining

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Testes were fixed in 4% PFA, embedded in Tissue-Tek O.C.T. compound, and cut section with 5 μm thick. Sperm were fixed in 4% PFA and permeated by 0.3% Triton X-100. TUNEL staining was performed using the TUNEL ApoGreen Detection Kit (YEASEN, 40307ES20, China). Images were obtained with a FluoView 1000 microscope (Olympus, Japan).

Western blot

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HEK293T cells (Cat# GNHu43, obtained from Stem cell Bank of Chinese Academic Science, the identity has been authenticated using Short Tandem Repeat profiling) were tested for mycoplasma contamination and showed negative result. Fresh mouse adult testes or HEK293T cells were collected, and proteins were extracted by using RIPA (Radioimmunoprecipitation assay) buffer (Beyotime, P0013J, China). In total, 40 μg of protein lysates were separated on a 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) gel, proteins were transferred to PVDF (Polyvinylidene Fluoride) membranes (Bio-Rad) and the membranes were blocked in 5% non-fat milk (blocking solution) for 1 hr. Primary antibodies were incubated overnight at 4°C after blocking. The membranes were washed with TBST three times and then incubated with a secondary antibody for 1 hr before using Luminol/enhancer solution and Clarity Western ECL Substrate (Bio-Rad Laboratories, Inc US). Western blot images were scanned by using Gel Doc XR system (Bio-Rad Laboratories, Inc US).

IP assays

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For testis tissue IP experiments, the testes were dissected and lysed in IP buffer (Beyotime, P0013J, China), clarified by centrifugation at 12,000 × g, and then pre-cleared with anti-HA nanobody agarose beads (AlpaLife, ktsm1305, China). The lysate was incubated with primary antibodies overnight at 4°C on a rotator and conjugated with anti-HA beads. The beads were washed with IP buffer and then boiled in 2× SDS loading buffer for western blotting analysis.

For cell line IP experiments, the HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 (Life Technologies). After 48 hr, IP was performed as described previously (Li et al., 2022). 25 μl mCherry-Trap bead 50% slurry (AlpaLife, ktsm1331, China) was used and all wash steps were performed with washing buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 0.5 mM Ethylenediaminetetraacetic Acid). mCherry-Trap beads were washed with dilution buffer prior to addition to the cell lysate. Beads were incubated with cell lysate at 4°C for 2 hr following another wash step. To elute the proteins from the beads, 40 μl sample buffer (120 mM Tris–HCl pH 6.8, 20% glycerol, 4% SDS, 0.04% Bromophenol blue, 10% β-mercaptoehanol) was added and boiled at 95°C for 5 min. mCherry-tagged proteins were detected by western blotting using an anti-mCherry antibody.

Spermatogenic cell isolation

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Spermatogenic cells were isolated from the whole mouse testis using the STA-PUT method described previously with slight modifications (Bellvé, 1993). In brief, testes were collected from 8-week-old mice WT and Fbxo24 KO mice and decapsulated with collagenase treatment to remove Leydig cells. The dispersed seminiferous tubules were then digested with trypsin and DNase I to single-cell suspensions. Next, Sertoli cells were separated from germ cells by filtration through a 40-mm cell strainer and by adhesion to lectin-coated culture plates. Store the cells in the 1× Krebs buffer on ice. Different germ cell populations were separated using a manually prepared 0.5–5% discontinuous BSA (Bovine Serum Albumin) density gradient for velocity sedimentation sediment. After sedimentation (1.5 hr, 4°C), enriched fractions of the cell were manually collected, and the cell concentration was determined.

RNA isolation and quantitative qPCR

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Total RNAs were extracted from purified germ cell fractions using TRIzol reagent (Invitrogen, UK) following the manufacturer’s procedure. The purity and concentration of RNA samples were determined using a Nanodrop ND-2000 spectrophotometer (Thermo Scientific, Madison, USA). Reverse transcription of 500 ng purified total RNA was performed by PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China). RT-qPCR was performed with SYBR green master mix (Takara, Dalian, China) on the ABI Step One System (Applied Biosystems) according to the manufacturers’ procedure. The relative gene expression was quantified using the comparative cycle threshold method, with the Gapdh expression used for normalization.

RNA-seq analysis

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1 μg of total RNA was used from each group to prepare the mRNA libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat. No. RS-122-2101, Illumina) according to the manufacturer’s instructions. All libraries were sequenced using the Illumina HiSeq 4000 platform. The FASTX-Toolkit was used to remove adaptor sequences, and low-quality reads from the sequencing data. To identify all the transcripts, we used Tophat2 and Cufflinks to assemble the sequencing reads based on the UCSC mm10 mouse genome. The differentially expression analysis was performed by Cuffdiff. The differential expressed genes were set with the threshold of p < 0.05 and fold change >2.

Small RNA-seq and annotation

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For small RNA-seq, the total RNA from adult testes was gel fractionated, and those 16–40 nt in length were enriched by polyacrylamide gel electrophoresis, and purified for small RNA-seq. The small RNA libraries were constructed using the Digital Gene Expression for Small RNA Sample prep kit (Illumina). Sequencing of the small RNA library was performed by Illumina HiSeq Xten. Small RNA annotation was performed as described previously (Kuramochi-Miyagawa et al., 2008).

Statistical analysis

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All data are presented as mean ± standard deviation unless otherwise noted in the figure legends. Statistical differences between datasets were assessed by one-way analysis of variance or Student’s t-test using the GraphPad Prism 8 software. p-values are denoted in figures by *p < 0.05; **p < 0.01; ***p < 0.001.

Data availability

RNA-seq and small RNA-seq data are deposited in the NCBI SRA database with the accession number PRJNA878933 and PRJNA878953, respectively.

The following data sets were generated
    1. Li Z
    (2022) NCBI BioProject
    ID PRJNA878933. RNA-seq data of FBXO24 KO mouse.
    https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA878933
    1. Li Z
    (2022) NCBI BioProject
    ID PRJNA878953. Small RNA-seq of FBXO24KO mouse.
    https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA878953

References

Article and author information

Author details

  1. Zhiming Li

    Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Contribution
    Conceptualization, Data curation, Software, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Methodology, Writing – original draft
    For correspondence
    lzmleo@hust.edu.cn
    Competing interests
    No competing interests declared

  2. Xingping Liu

    Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Contribution
    Formal analysis, Investigation
    Competing interests
    No competing interests declared

  3. Yan Zhang

    Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Contribution
    Investigation
    Competing interests
    No competing interests declared

  4. Yuanyuan Li

    Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Contribution
    Investigation
    Competing interests
    No competing interests declared

  5. Liquan Zhou

    Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Contribution
    Supervision, Funding acquisition, Validation, Investigation
    For correspondence
    zhouliquan@hust.edu.cn
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9332-9408

  6. Shuiqiao Yuan

    1. Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    2. Laboratory of Animal Center, Huazhong University of Science and Technology, Wuhan, China
    Contribution
    Conceptualization, Funding acquisition, Writing – review and editing
    For correspondence
    shuiqiaoyuan@hust.edu.cn
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1460-7682

Funding

National Natural Science Foundation of China (82001620)

  • Zhiming Li

National Natural Science Foundation of China (82171605)

  • Shuiqiao Yuan

National Key Research and Development Program of China (2018YFC1004500)

  • Liquan Zhou

Open Research Fund of the National Center for Protein Sciences at Peking University in Beijing (KF-202205)

  • Zhiming Li

Open Research Fund of Key Laboratory of Reproductive Medicine of Guangdong Province (2020B1212060029)

  • Zhiming Li

National Natural Science Foundation of China (82371627)

  • Zhiming Li

The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.

Acknowledgements

This work was supported by National Natural Science Foundation of China (82001620, 82371627 to ZL and 82171605 to SY), the National Key R&D Program of China (2018YFC1004500 to LZ), the Open Research Fund of the National Center for Protein Sciences at Peking University in Beijing (KF-202205 to ZL), and the Open Research Fund of Key Laboratory of Reproductive Medicine of Guangdong Province (2020B1212060029 to ZL).

Ethics

All the animal procedures were approved by the Institutional Animal Care and Use Committee protocols (Permit Number: S2795) of Tongji Medical College, Huazhong University of Science and Technology, and the mice were housed in the specific pathogen-free facility of Huazhong University of Science and Technology. All experiments with mice were conducted ethically according to the Guide for the Care and Use of Laboratory Animal guidelines.

Version history

  1. Sent for peer review: September 12, 2023
  2. Preprint posted: October 1, 2023 (view preprint)
  3. Preprint posted: November 22, 2023 (view preprint)
  4. Preprint posted: February 28, 2024 (view preprint)
  5. Version of Record published: March 12, 2024 (version 1)

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You can cite all versions using the DOI https://doi.org/10.7554/eLife.91666. This DOI represents all versions, and will always resolve to the latest one.

Copyright

© 2023, Li et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. Zhiming Li
  2. Xingping Liu
  3. Yan Zhang
  4. Yuanyuan Li
  5. Liquan Zhou
  6. Shuiqiao Yuan
(2024)
FBXO24 modulates mRNA alternative splicing and MIWI degradation and is required for normal sperm formation and male fertility
eLife 12:RP91666.
https://doi.org/10.7554/eLife.91666.3

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    Arne Elofsson, Ling Han ... Luca Jovine
    Research Article

    A crucial event in sexual reproduction is when haploid sperm and egg fuse to form a new diploid organism at fertilization. In mammals, direct interaction between egg JUNO and sperm IZUMO1 mediates gamete membrane adhesion, yet their role in fusion remains enigmatic. We used AlphaFold to predict the structure of other extracellular proteins essential for fertilization to determine if they could form a complex that may mediate fusion. We first identified TMEM81, whose gene is expressed by mouse and human spermatids, as a protein having structural homologies with both IZUMO1 and another sperm molecule essential for gamete fusion, SPACA6. Using a set of proteins known to be important for fertilization and TMEM81, we then systematically searched for predicted binary interactions using an unguided approach and identified a pentameric complex involving sperm IZUMO1, SPACA6, TMEM81 and egg JUNO, CD9. This complex is structurally consistent with both the expected topology on opposing gamete membranes and the location of predicted N-glycans not modeled by AlphaFold-Multimer, suggesting that its components could organize into a synapse-like assembly at the point of fusion. Finally, the structural modeling approach described here could be more generally useful to gain insights into transient protein complexes difficult to detect experimentally.

    1. Developmental Biology

    Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis

    Amandine Jarysta, Abigail LD Tadenev ... Basile Tarchini
    Research Article

    Inhibitory G alpha (GNAI or Gαi) proteins are critical for the polarized morphogenesis of sensory hair cells and for hearing. The extent and nature of their actual contributions remains unclear, however, as previous studies did not investigate all GNAI proteins and included non-physiological approaches. Pertussis toxin can downregulate functionally redundant GNAI1, GNAI2, GNAI3, and GNAO proteins, but may also induce unrelated defects. Here, we directly and systematically determine the role(s) of each individual GNAI protein in mouse auditory hair cells. GNAI2 and GNAI3 are similarly polarized at the hair cell apex with their binding partner G protein signaling modulator 2 (GPSM2), whereas GNAI1 and GNAO are not detected. In Gnai3 mutants, GNAI2 progressively fails to fully occupy the sub-cellular compartments where GNAI3 is missing. In contrast, GNAI3 can fully compensate for the loss of GNAI2 and is essential for hair bundle morphogenesis and auditory function. Simultaneous inactivation of Gnai2 and Gnai3 recapitulates for the first time two distinct types of defects only observed so far with pertussis toxin: (1) a delay or failure of the basal body to migrate off-center in prospective hair cells, and (2) a reversal in the orientation of some hair cell types. We conclude that GNAI proteins are critical for hair cells to break planar symmetry and to orient properly before GNAI2/3 regulate hair bundle morphogenesis with GPSM2.

    1. Computational and Systems Biology
    2. Developmental Biology

    A logic-incorporated gene regulatory network deciphers principles in cell fate decisions

    Gang Xue, Xiaoyi Zhang ... Zhiyuan Li
    Research Article

    Organisms utilize gene regulatory networks (GRN) to make fate decisions, but the regulatory mechanisms of transcription factors (TF) in GRNs are exceedingly intricate. A longstanding question in this field is how these tangled interactions synergistically contribute to decision-making procedures. To comprehensively understand the role of regulatory logic in cell fate decisions, we constructed a logic-incorporated GRN model and examined its behavior under two distinct driving forces (noise-driven and signal-driven). Under the noise-driven mode, we distilled the relationship among fate bias, regulatory logic, and noise profile. Under the signal-driven mode, we bridged regulatory logic and progression-accuracy trade-off, and uncovered distinctive trajectories of reprogramming influenced by logic motifs. In differentiation, we characterized a special logic-dependent priming stage by the solution landscape. Finally, we applied our findings to decipher three biological instances: hematopoiesis, embryogenesis, and trans-differentiation. Orthogonal to the classical analysis of expression profile, we harnessed noise patterns to construct the GRN corresponding to fate transition. Our work presents a generalizable framework for top-down fate-decision studies and a practical approach to the taxonomy of cell fate decisions.