Soil-borne fungi alter the apoplastic purinergic signaling in plants by deregulating the homeostasis of extracellular ATP and its metabolite adenosine
- ETH Zurich, Switzerland
- University of Zurich, Switzerland
Abstract
Purinergic signaling activated by extracellular nucleotides and their derivative nucleosides trigger sophisticated signaling networks. The outcome of these pathways determine the capacity of the organism to survive under challenging conditions. Both extracellular ATP (eATP) and Adenosine (eAdo) act as primary messengers in mammals, essential for immunosuppressive responses. Despite the clear role of eATP as a plant damage-associated molecular pattern, the function of its nucleoside, eAdo, and of the eAdo/eATP balance in plant stress response remain to be fully elucidated. This is particularly relevant in the context of plant-microbe interaction, where the intruder manipulates the extracellular matrix. Here, we identify Ado as a main molecule secreted by the vascular fungus Fusarium oxysporum. We show that eAdo modulates the plant's susceptibility to fungal colonization by altering the eATP-mediated apoplastic pH homeostasis, an essential physiological player during the infection of this pathogen. Our work indicates that plant pathogens actively imbalance the apoplastic eAdo/eATP levels as a virulence mechanism.
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All data generated or analysed during this study are included in the manuscript and supporting file.
Article and author information
Author details
Consuelo M De Moraes
Clara Sanchez-Rodriguez
Funding
Peter und Traudl Engelhorn Stiftung
- Christopher Kesten
ETH Zürich Foundation (SEED-05 19-2)
- Christopher Kesten
Novo Nordisk Foundation (NNF20OC0060564)
- Christopher Kesten
Lundbeck Foundation (R346-2020-1546)
- Christopher Kesten
Swiss National Science Foundation (31003A_182625)
- Cyril Zipfel
European Molecular Biology Organization (683-2018)
- Julian Dindas
Swiss National Science Foundation (310030_184769)
- Clara Sanchez-Rodriguez
ETH Zürich
- Consuelo M De Moraes
ETH Zürich
- Clara Sanchez-Rodriguez
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2023, Kesten et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Soil-borne fungi alter the apoplastic purinergic signaling in plants by deregulating the homeostasis of extracellular ATP and its metabolite adenosine
eLife 12:e92913.
https://doi.org/10.7554/eLife.92913
