Figure 2—figure supplement 1. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 2—figure supplement 1.

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California Institute of Technology, United States
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. Verification of zebrafish dbh mutant.

(A) Alignment of human, mouse, WT zebrafish and mutant zebrafish DBH amino acid sequences. The coding sequence of the mutant allele terminates before the conserved active site residue (asterisk). (B) ELISA quantification of NE levels in WT and dbh−/ zebrafish larvae. Mean ± s.e.m. is shown. ***, p < 0.0001 by one-way ANOVA. (C) RNA in situ hybridization (ISH) for dbh in dbh+/ (C′) and dbh−/ (C′′) sibling larvae in the LC, indicated by white arrows. Larvae were obtained from a dbh+/ to dbh−/ mating and were genotyped after imaging. Schematic diagram indicates the brain region (box) containing the LC (green) that is shown in the ISH images. a = anterior, p = posterior. Scale bar = 50 μm.

DOI: http://dx.doi.org/10.7554/eLife.07000.008