Figure 5—figure supplement 1. | Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior in C. elegans

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Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior in C. elegans

Figure 5—figure supplement 1.

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Massachusetts Institute of Technology, United States
Figure 5—figure supplement 1.
Download figureOpen in new tabFigure 5—figure supplement 1. P. aeruginosa influences exploratory behavior and daf-7 expression in both males and hermaphrodites.

(A) Representative leaving curves for males (dashed) and hermaphrodites (solid) on E. coli (shades of grey) and P. aeruginosa (shades of green). All strains were fed in this experiment. (B) Probability of leaving values for males and hermaphrodites on E. coli and P. aeruginosa. All strains were fed in these experiments. Values plotted are the mean + SEM for two independent experiments, n = 40 animals for all conditions. *p<0.05 as determined by unpaired t-test with Welch’s correction. n.s., not significant. (C) Representative raw data curves for starved hermaphrodites on E. coli and P. aeruginosa. Fed hermaphrodites shown as control. (D) Maximum fluorescence of pdaf-7::gfp in the ASJ neurons of starved hermaphrodites re-fed E. coli or P. aeruginosa for four hours (compare to Figure 5B). Error bars indicate SD. (E) Maximum fluorescence values of pdaf-7::gfp in the ASJ neurons of fed and starved animals and animals that were starved for a period of 24 hr and then reintroduced to P. aeruginosa for the indicated amount of time. The experiment in Figure 4B was run in parallel, controls (fed and starved) were shared between conditions. These controls are shown in both figures for comparison purposes. *p<0.05 and ***p<0.001, as determined by ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. Error bars indicate S.D.

DOI: http://dx.doi.org/10.7554/eLife.21166.013