The ability of epithelial cells to distinguish between domains on opposing cell surfaces within a tissue, a property known as planar cell polarity, relies on proteins and protein complexes directing the traffic of signaling proteins to specific locations on the cell surface membrane.
Hung-Hsiang Huang, Antti Hassinen ... Pamela Stanley
GnT1IP-L is a membrane bound glycoprotein that interacts with MGAT1 in the Golgi, but not in the endoplasmic reticulum, to regulate complex N-glycan synthesis.
Association of curvature generating proteins to the Golgi membranes by sphingomyelin metabolism essentially controls the flatness of a Golgi cisterna that is necessary for efficient sorting and export.
Galina V Beznoussenko, Seetharaman Parashuraman ... Alberto Luini
The soluble cargo protein albumin traverses the Golgi stack at much faster rates than procollagen aggregates, by diffusion via intercisternal continuities.
Pak-yan Patricia Cheung, Charles Limouse ... Suzanne R Pfeffer
Atomic force microscopy reveals a floppy, dimeric, coiled coil Golgin structure that captures transport vesicles via splayed, N-terminal ends at the Golgi complex.
Imaging, quantitative immunoblotting and mass spectrometry reveal that hundreds of surface-expressed neuronal membrane proteins exhibit atypical glycosylation profiles, resulting in changes in protein half-life and synaptic responses.