A fluorometric analysis under voltage clamp shows the presence of a converged electric field in P2X2 channel with no canonical voltage-sensor and identifies the structural determinants for the voltage-dependent gating.
Keenan C Taylor, Po Wei Kang ... Charles R Sanders
The intermediate state conformation of the human KCNQ1 potassium channel voltage sensor domain was determined, validated, and shown to be conductive under physiological conditions.
Skeletal muscle cells constantly monitor their own activity and that of their partner neuron at synapses, enabling them to provide the neuron with feedback regarding neurotransmitter release.
Optogenetic tools enable sophisticated measurements of a voltage-gated sodium channel implicated in pain, as well as high-throughput screening of candidate channel blockers.
Michaela A Edmond, Andy Hinojo-Perez ... Rene Barro-Soria
Because voltage-gated KCNQ2 channels are central to physiological and pathophysiological events, understanding how disease-causing mutations in different channel regions disrupt function will help future development of mutation-specific antiepileptic therapies.
Direct patch clamp of ependymal motile cilia reveals that voltage-gated calcium channels in the cell body dominate their electrical and calcium signaling properties.
A powerful new fluorescence approach elucidates the structural mechanism for a specialized ion channel behavior important for cardiac and neuronal excitability.
Jeremy S Treger, Michael F Priest, Francisco Bezanilla
ArcLight, a popular optogenetic reporter of voltage, is studied at both single-molecule and macroscopic levels, which leads to new mechanistic understanding and to the rational design of a faster reporter.
Cyrrus M Espino, Cheyanne M Lewis ... Theanne N Griffith
The voltage-gated sodium channel Nav1.1 is identified as an essential component of the proprioceptive transmission machinery that is required in vivo for normal motor behavior.
