High-throughput single-cell based, genome-wide gene silencing screening (sCRilecs-seq) revealed that drugging the mevalonate pathway resensitizes amoxicillin-resistant pneumococci.

Selection by national-level antibiotic consumption and vaccination trends drives atypical recombinations, frequently resulting in interspecies sequence exchange at many sites across a bacterial species’ chromosome.

The mathematical modeling approach disentangles the impacts of the COVID-19 pandemic on antibiotic resistance in Streptococcus pneumoniae in the community setting and identifies the three most plausible driving mechanisms responsible for the observed trends in invasive isolates and pneumococcal carriage.

Tracking fluorescent fusion proteins in competent pneumococcal cells reveals a polar hub for competence regulation, with the alternative sigma factor σX relocalizing DprA to this hub to mediate competence shut-off.

Fc engineering of monoclonal antibodies strongly improves immune clearance of pneumococcus.

Panproteome array analysis of antibody responses to a pneumococcal whole cell vaccine reveals consistent induction of immune responses to multiple surface proteins, despite individuals' unique pre-existing antibody profiles.

Selective removal of Wall Teichoic Acids from the envelope of Streptococcus pneumoniae prevents bacteriolysis and directs zonal cell wall synthesis.

Long Pentaxin 3 is highly expressed by non-hematopoietic cells during Streptococcus pneumoniae invasive infections and regulates polymorphonuclear neutrophils' recruitment through interaction with P-selectin which damps inflammation-associated tissue damage and pneumococcal systemic dissemination.

Intranasal immunization of inactivated whole cell of some Gram-negative bacteria induces very rapid and efficient protection against bacterial pulmonary by training alveolar macrophage response, which can be harnessed to design rapid-effecting vaccine against multidrug-resistant bacteria infection.

Future vaccination strategies in pneumococcal disease should target specific bacterial serotypes for different host age groups.