Generation of stable transgenic opsin lines together with in vivo calibration of their efficacy using behavioural and electrophysiological assays constitutes a novel optogenetic toolkit in zebrafish.
Melanopsin phototransduction targets distinct complements of transduction channels across intrinsically photosensitive retinal ganglion cell subtypes and does not require hyperpolarization-activated cyclic nucleotide-gated channels.
Johannes Oppermann, Andrey Rozenberg ... Peter Hegemann
Engineered anion-conducting channelrhodopsins with enhanced red-light sensitivity and accelerated kinetics enable precise, low-intensity optical silencing of neurons, advancing optogenetic control in neuroscience research.
The atomic structure of GtACR1 provides new insight into the chemical mechanism of natural light-gated anion membrane conductance, and enables its optimization for optogenetic photoinhibition of neuron firing.
A viral heliorhodopsin from Emiliania huxleyi virus 202 (V2HeR3) is a light-activated proton transporter, which has the potential to depolarize the host cells by light, possibly to overcome the host defense mechanisms or to prevent superinfection.
A new soma-targeted variant of the large-conductance blue-light-sensitive opsin CoChR combined with advanced optical stimulation methods allows high-efficiency all-optical neuronal imaging and stimulation in mouse brain in vivo.
A high axonal chloride concentration explains why activation of light-gated chloride channels causes neurotransmitter release, and a novel hybrid somatodendritic targeting motif ameliorates this phenomenon and improves their inhibitory function.
Quantitative models for responses of rod and cone photoreceptors are developed that allow direct tests of the impact of the photoreceptors on responses of downstream visual neurons.
