A bacterial sulfonolipid triggers multicellular development in the closest living relatives of animals

  1. Rosanna A Alegado
  2. Laura W Brown
  3. Shugeng Cao
  4. Renee K Dermenjian
  5. Richard Zuzow
  6. Stephen R Fairclough
  7. Jon Clardy  Is a corresponding author
  8. Nicole King  Is a corresponding author
  1. University of California, Berkeley, United States
  2. Harvard Medical School, United States
  3. Stanford University School of Medicine, United States

Abstract

Bacterially-produced small molecules exert profound influences on animal health, morphogenesis, and evolution through poorly understood mechanisms. In one of the closest living relatives of animals, the choanoflagellate Salpingoeca rosetta, we find that rosette colony development is induced by the prey bacterium Algoriphagus machipongonensis and its close relatives in the Bacteroidetes phylum. Here we show that a rosette inducing factor (RIF-1) produced by A. machipongonensis belongs to the small class of sulfonolipids, obscure relatives of the better known sphingolipids that play important roles in signal transmission in plants, animals, and fungi. RIF-1 has extraordinary potency (femtomolar, or 10−15 M) and S. rosetta can respond to it over a broad dynamic range—nine orders of magnitude. This study provides a prototypical example of bacterial sulfonolipids triggering eukaryotic morphogenesis and suggests molecular mechanisms through which bacteria may have contributed to the evolution of animals.

https://doi.org/10.7554/eLife.00013.001

eLife digest

All animals, including humans, evolved in a world filled with bacteria. Although bacteria are most familiar as pathogens, some bacteria produce small molecules that are essential for the biology of animals and other eukaryotes, although the details of the ways in which these bacterial molecules are beneficial are not well understood.

The choanoflagellates are water-dwelling organisms that use their whip-like flagella to move around, feeding on bacteria. They can exist as one cell or a colony of multiple cells and, perhaps surprisingly, are the closest known living relatives of animals. This means that experiments on these organisms have the potential to improve our understanding of animal development and the transition from egg to embryo to adult.

Alegado et al. have explored how the morphology of Salpingoeca rosetta, a colony-forming choanoflagellate, is influenced by its interactions with various species of bacteria. In particular, they find that the development of multicellularity in S. rosetta is triggered by the presence of the bacterium Algoriphagus machipongonensis as well as its close relatives. They also identify the signaling molecule produced by the bacteria to be C32H64NO7S; this lipid molecule is an obscure relative of the sphingolipid molecules that have important roles in signal transmission in animals, plants, and fungi. Moreover, Alegado et al. show that S. rosetta can respond to this molecule – which they call rosette-inducing factor (RIF-1) – over a wide range of concentrations, including concentrations as low as 10−17 M.

The work of Alegado et al. suggests that interactions between S. rosetta and Algoriphagus bacteria could be a productive model system for studying the influences of bacteria on animal cell biology, and for investigating the mechanisms of signal delivery and reception. Moreover, the molecular mechanisms revealed by this work leave open the possibility that bacteria might have contributed to the evolution of multicellularity in animals.

https://doi.org/10.7554/eLife.00013.002

Introduction

Eukaryotes evolved in a world filled with bacteria and throughout their shared history these two branches of life have developed a complex set of ways to compete and cooperate with each other. While research on these interactions has historically emphasized bacterial pathogens, bacteria also regulate the biology of eukaryotes in many other ways (McFall-Ngai 1999; Koropatnick et al. 2004; Mazmanian et al. 2005; Falkow 2006; Hughes and Sperandio 2008; Desbrosses and Stougaard 2011) and may have exerted critical influences on animal evolution. Choanoflagellates, microscopic bacteria-eating eukaryotes that are the closest living relatives of animals (James-Clark 1868; Saville Kent 1880; Hibberd 1975; Carr et al. 2008; King et al. 2008; Ruiz-Trillo et al. 2008), could provide particularly important insights into the mechanisms underlying bacterial influences on animal biology and evolution. Moreover, some choanoflagellates have both solitary and multicellular stages in their life histories (Leadbeater 1983; Karpov and Coupe 1998; Dayel et al. 2011) and understanding the environmental cues that regulate choanoflagellate colony formation could provide a molecular model for animal multicellularity.

Results

In the choanoflagellate Salpingoeca rosetta, rosette-shaped multicellular colonies develop when a single founder cell undergoes multiple rounds of incomplete cytokinesis, leaving neighboring cells physically attached by fine intercellular bridges (Fairclough et al. 2010; Dayel et al. 2011). Although the original stock of S. rosetta (ATCC50818) was established from a rosette colony (Dayel et al. 2011), laboratory cultures consistently produced single cells, with small numbers of rosette colonies forming only sporadically (Figure 1A, Figure 1—figure supplement 1). Serendipitously, we discovered that the bacterial community influences rosette colony development. Treatment of the ATCC50818 culture with an antibiotic cocktail resulted in a culture of S. rosetta cells that proliferated robustly by feeding on the remaining antibiotic-resistant bacteria but never formed rosette colonies, even upon removal of antibiotics (Figure 1B). This culture line is hereafter referred to as RCA (for ‘Rosette Colonies Absent’). Supplementation of RCA cultures with bacteria from ATCC50818 restored rosette colony development, revealing that S. rosetta cells in the RCA culture remained competent to form colonies and would do so when stimulated by the original community of environmental bacteria.

Figure 1 with 1 supplement see all
Rosette colony development in S. rosetta is regulated by A. machipongonensis.

(A) The original culture of S. rosetta, ATCC 50818, contains diverse co-isolated environmental bacteria and forms rosette colonies (arrowheads) rarely. (B) Treatment of ATCC50818 with a cocktail of antibiotics reduced the bacterial diversity and yielded an S. rosetta culture line, RCA, in which rosette colonies never formed. (Representative single cells indicated by arrows.) (C) Addition of A. machipongonensis to RCA cultures was sufficient to induce rosette development. Scale bar, 2 μm.

https://doi.org/10.7554/eLife.00013.003
Table 1

Species tested for colony induction

https://doi.org/10.7554/eLife.00013.005
Species16S rDNA accession numberReferenceRosette colonies
Algoriphagus machipongonensis PR1NZ_AAXU00000000Alegado et al. (2012)+
Algoriphagus alkaliphilus AC-74AJ717393Tiago et al. (2006)+
Algoriphagus boritolerans T-22AB197852Ahmed et al. (2007)+
Algoriphagus mannitolivorans JC2050AY264838Yi and Chun (2004)+
Algoriphagus marincola SW-2AY533663Yoon et al. (2004)+
Algoriphagus ornithinivorans JC2052AY264840Yi and Chun (2004)+
Algoriphagus vanfongensis KMM 6241EF392675Van Trappen et al. (2004)+
Algoriphagus antarcticus LMG 21980AJ577141Nedashkovskaya et al. (2004)+
Algoriphagus aquimarinus LMG 21971AJ575264Nedashkovskaya et al. (2004)+
Algoriphagus chordae LMG 21970AJ575265Nedashkovskaya et al. (2004)+
Algoriphagus halophilus JC2051AY264839Yi and Chun (2004)+
Algoriphagus locisalis MSS-170AY835922Yoon et al. (2005a)+
Algoriphagus ratkowskyi LMG 21435AJ608641Bowman et al. (2003)+
Algoriphagus terrigena DS-44DQ178979Yoon et al. (2006)+
Algoriphagus winogradskyi LMG 21969AJ575263Nedashkovskaya et al. (2004)+
Algoriphagus yeomjeoni MSS-160AY699794Yoon et al. (2005b)+
Agrobacterium tumefaciens C58AE007870Wood et al. (2001)+
Aquiflexum balticum BA160AJ744861Brettar et al. (2004a)
Bacillus subtilis 168AL009126Kunst et al. (1997); Burkholder and Giles (1947); Spizizen (1958)
Bacteroides fragilis NCTC9343CR626927Cerdeno-Tarraga et al. (2005)
Belliella baltica BA134AJ564643Brettar et al. (2004b)
Caulobacter crescentus CB15AE005673Nierman et al. (2001)
Croceibacter atlanticus HTCC2559NR_029064Cho and Giovannoni (2003)
Cyclobacterium marinum LMG 13164AJ575266Raj and Maloy 1990)+
Cytophaga hutchinsonii ATCC 33406M58768Lewin (1969)+
Dyadobacter fermentans DSM 18053NR_027533Chelius and Triplett (2000)+
Echinicola pacifica KMM 6172NR_043619Nedashkovskaya et al. (2006)
Escherichia coli MG1655U00096Blattner et al. (1997)
Flavobacteria johnsoniae UW101CP000685Bernardet et al. (1996)
Flectobacillus major DSM 103M62787Raj and Maloy (1990)+
Listeria monocytogenes 10403SCP002002Edman et al. (1968)
Magnetospirillum magneticum AMB-1AP007255Matsunaga et al. (2005)
Microscilla marina ATCC 23134M123134Garrity (2010)
Oceanostipes pacificus HTCC2170CP002157Oh et al. (2011)
Robiginitalea biformata HTCC2501CP001712Cho and Giovannoni (2004)
Salinibacter ruber DSM13855CP000159Anton et al. (2002)
Sphingomonas wittichii RW1CP000699Miller et al. (2010)
Vibrio fischeri ES114CP000021Ruby et al. (2005)
Zobellia galactonovorans DsijNR_025053Barbeyron et al. (2001)+
Zobellia uliginosa ATCC 14397M62799Matsuo et al. (2003)+
  1. −: no rosette colonies observed; +: rosette colonies observed.

To determine which co-isolated bacterial species stimulate rosette colony development in S. rosetta, the RCA cell line was supplemented with 64 independent bacterial isolates from ATCC50818 and monitored for the appearance of rosette colonies. Only one bacterial species from ATCC50818, the previously undescribed Algoriphagus machipongonensis (phylum Bacteroidetes; Alegado et al. 2012), induced rosette colony development in the RCA cell line (Figure 1C). S. rosetta cultures fed solely with A. machipongonensis yielded high percentages of rosette colonies (Figure 1—figure supplement 1), demonstrating that no other co-isolated bacterial species is required to stimulate rosette colony development.

What was not clear was whether other bacteria might also be competent to induce rosette colony development. Therefore, representative Bacteroidetes and non-Bacteroidetes bacteria were grown and fed to RCA cultures (Figure 2, Table 1). None of the non-Bacteroidetes species tested, including members of the γ-proteobacteria, α-proteobacteria, and Gram-positive bacteria, were competent to induce rosette colony development. In contrast, all 15 Algoriphagus species tested induced rosette colony development, as did six of 16 other closely related species tested in the Bacteroidetes phylum (Table 1). Therefore, the ability to induce rosette colony development is enriched in Algoriphagus bacteria and their relatives.

Diverse members of the Bacteroidetes phylum induce rosette colony development.

A maximum likelihood phylogeny inferred from 16S rDNA gene sequences reveals the evolutionary relationships among A. machipongonensis, other members of the Bacteroidetes phylum, and representative γ-proteobacteria (γ), α-proteobacteria (α), and Gram-positive (+) bacteria. All 15 members of the Algoriphagus genus (Table 1), as well as six other species in the Bacteroidetes phylum, were competent to induce colony development (filled squares). In contrast, no species outside of Bacteroidetes and most of the non-Algoriphagus bacteria tested failed to induce rosette colony development (open squares). Scale bar, 0.1 substitutions per nucleotide position.

https://doi.org/10.7554/eLife.00013.006

Although Bacteroidetes bacteria regulate morphogenetic processes in such diverse lineages as animals, red algae, and green algae (Provasoli and Pintner 1980; Matsuo et al. 2005; Mazmanian et al. 2005), the bacterially produced chemical cues that regulate most of these partnerships remain obscure. The limited phylogenetic distribution of bacteria capable of inducing rosette colony development suggested that A. machipongonensis and its close relatives may produce a characteristic molecule that could be identified biochemically. The complete absence of rosette colonies in RCA cultures provided the basis for a robust bioassay that we developed to identify the rosette-inducing molecule(s), which we named RIFs (Rosette-Inducing Factors), from A. machipongonensis cultures. Preliminary studies demonstrated that RIF activity was present in conditioned medium from A. machipongonensis, even when grown in the absence of choanoflagellates. Furthermore, the activity was also found in the A. machipongonensis cell envelope and was heat, protease, and nuclease resistant, revealing that the compound is not a protein, RNA, or DNA (Table 2).

Table 2

Responses of RCA culture to various supplements

https://doi.org/10.7554/eLife.00013.007
TreatmentRosette coloniesInterpretation
Sea water
CM from ATCC50818+RIF-1 present in environmental isolate ATCC 50818
CM from RCARIF-1 is absent in RCA lines
Live A. machipongonensis (cell pellet)++RIF-1 is produced by A. machipongonensis
Heat killed A. machipongonensis (cell pellet)++RIF-1 is resistant to heat
A. machipongonensis CM+RIF-1 is released by live Algoriphagus
A. machipongonensis CM, boiled 10 min+RIF-1 is not labile
A. machipongonensis CM + Proteinase K+RIF-1 is not a protein
A. machipongonensis CM + DNAse+RIF-1 is not DNA
A. machipongonensis CM + RNAse+RIF-1 is not RNA
A. machipongonensis CM, MeOH extract+RIF-1 is an organic compound
A. machipongonensis cell pellet, MeOH extract++RIF-1 is present in the Algoriphagus cell envelope
A. machipongonensis cell pellet, Bligh-Dyer extract++RIF-1 is a lipid
Sphingomyelin (20 mg mL−1)Sphingomyelin does not induce rosette colony development
Monosialoganglioside (20 mg mL−1)Monosialoganglioside does not induce rosette colony development
Galactocerebroside (20 mg mL−1)Galactocerebroside does not induce rosette colony development
N-palmitoyl-DL-dihydrolacto cerebroside (20 mg mL−1)N-palmitoyl-DL-dihydrolacto cerebroside does not induce rosette colony development
  1. −: no induction; +: low induction; ++: high induction

  2. CM: conditioned medium; RCA: rosette colonies absent; RIF-1: rosette inducing factor 1.

The bacterial cell envelope components lipopolysaccharide (LPS) and peptidoglycan (PGN) from Gram-negative bacteria have long been known to affect host biology (Cohn and Morse 1960; Hoffmann et al. 1999; Kopp and Medzhitov 1999; Takeuchi et al. 1999; Medzhitov and Janeway 2000; Kimbrell and Beutler 2001; Koropatnick et al. 2004), but neither LPS nor PGN from A. machipongonensis triggered rosette development, alone or in combination (Figure 3A). Instead, we found that A. machipongonensis crude lipid extracts enriched in sphingolipids robustly induced rosette development (Figure 3A). In animals, sphingolipid signaling pathways regulate developmental processes such as cell death, survival, differentiation, and migration (Prieschl and Baumruker 2000; Pyne and Pyne 2000; Spiegel and Milstien 2000; Hannun et al. 2001; Merrill et al. 2001; Herr et al. 2003). Moreover, sphingolipids serve essential functions both as structural components of cell membranes and as signaling molecules in diverse eukaryotes (Hannich et al. 2011). In contrast, the phylogenetic distribution of sphingolipids in bacteria is largely limited to Bacteroidetes and Sphingomonas, where their endogenous functions are poorly understood (Olsen and Jantzen 2001; An et al. 2011).

Figure 3 with 15 supplements see all
RIF-1, a sulfonolipid that induces rosette colony development.

(A) Rosette colony development is induced by live A. machipongonensis and the sphingolipid-enriched lipid fraction (20 mg mL−1), but not by fresh medium, A. machipongonensis LPS (10 mg mL−1), PGN (50 mg mL−1), or LPS+PGN. Shown are the whisker-box plots of the % colonial cells/total cells under each condition in three independent experiments. (B) The molecular structure of RIF-1 deduced from MS and 1D- and 2D-NMR data. The RIF-1 structure, 3,5-dihydroxy-2-(2-hydroxy-13-methyltetradecanamido)-15-methylhexadecane-1-sulfonic acid, has two parts: a base (shown in red) that defines the capnine, and a fatty acid (shown in black). Features that distinguish RIF-1 from other known capnoids are shown with colored arrows: the 2-hydroxy on the fatty acid (black) and the 5-hydroxy on the capnine base (red).

https://doi.org/10.7554/eLife.00013.008

To isolate and characterize the molecule(s) underlying RIF activity, we focused on the fraction enriched in sphingolipids. Lipids isolated from 160 L of A. machipongonensis culture were separated using preparative liquid chromatography–mass spectrometry (LC-MS) and the activity of each fraction was measured using the rosette colony induction bioassay. RIF activity tracked with a single fraction, which was further purified by several rounds of preparative thin-layer chromatography (Figure 3—figure supplement 1) to yield approximately 700 µg of active compound (RIF-1) with sufficient purity for structural analysis. RIF-1 represents only 0.015% of the A. machipogonensis sphingolipid pool. Based on high-resolution mass spectrometry, RIF-1 has a molecular formula of C32H64NO7S (M-H: exptl. 606.44027, calcd. 606.44035, Figure 3—figure supplement 2). Detailed analysis of one- and two-dimensional (COSY, HMBC, TOCSY, Figure 3—figure supplements 3–15) nuclear magnetic resonance (NMR, 600 MHz, Table 3) spectra revealed the planar structure of RIF-1, an unusual sulfonolipid shown in Figure 3B.

Table 3

Table of NMR chemical shifts

https://doi.org/10.7554/eLife.00013.024
Positionδ 1H (multiplicity, J, #H)13C (δ, ppm)
NH8.21 (d, J=9.1 Hz, 1H)
13.01 (dd, J=14.2, 5.1 Hz, 1H)51.87
2.56 (dd, J=14.3, 3.6 Hz, 1H)
23.88 (ddd, J=13.0, 8.6, 4.5 Hz, 1H)50.89
33.78 – 3.71 (m, 1H)71.51
OH35.20 (d, J=4.2 Hz, 1H)
41.51 – 1.47 (m, 1H)41.36
1.33 – 1.29 (m, 1H)
53.61 – 3.53 (m, 1H)70.20
OH54.31 (d, J=3.5 Hz, 1H)
61.34 – 1.29 (m, 1H)37.27
1.24 – 1.20 (m, 1H)
7–131.21 – 1.27 (br s, 14H)22.5–29.6
141.16 – 1.11 (m, 2H)38.91
151.52 – 1.47 (m, 1H)27.79
16, 170.84 (d, J=6.6 Hz, 6H)22.09
1′173.23
2′3.80 (dd, J=6.6, 4.2 Hz, 1H)71.29
OH2′5.52 (d, J=5.0 Hz, 1H)
3′1.59 – 1.54 (m, 1H)34.85
1.50 – 1.45 (m, 1H)
4′?1.35 – 1.30 (m, 2H)24.99
5′–11′1.21 – 1.27 (br s, 14H)22.5–29.6
12′1.16 – 1.11 (m, 2H)38.91
13′1.52 – 1.47 (m, 1H)27.79
14′, 15′0.84 (d, J=6.6 Hz, 6H)22.09

Sulfonolipids like RIF-1 resemble sphingolipids, but there are important differences between the two. In sphingolipids, a sphingoid base (1,3-dihydroxy-2-aminoalkane) is linked through an amide bond to a fatty acid. The long alkyl chains of both the sphingoid base and fatty acid vary in length, branching, number of double bonds, and placement of hydroxyl substituents. In RIF-1, a capnoid base (2-amino-3-hydroxy-15-methyl-1-sulfonic acid) replaces the sphingoid base, and the sphingolipid hydroxyl, which is the attachment point for the major diversifying elements of the sphingolipid family, is replaced by a sulfonic acid. While members of the sphingolipid family, such as the ceramides, glycosphingolipids, sphingomyelins, and gangliosides, differ by the groups attached to the hydroxyl, the sulfonic acid function in sulfonolipids like RIF-1 has no reported diversifying modifications. In this sense sulfonolipid diversity appears more limited than sphingolipid diversity. Significantly, commercial sphingolipids (sphingomyelin, monosiloganglioside, galactocerebroside, and N-palmitoyl-DL-dihydrolacto cerebroside; Table 2) failed to show any activity in our assay system. To our knowledge, RIF-1 is the first sulfonolipid demonstrated to influence developmental processes in eukaryotes.

Finally, we investigated the potency of RIF-1 and its ability to induce colony development under plausible environmental conditions. Purified RIF-1 induces rosette formation with a bell-shaped dose-response curve over a broad range of concentrations, from 10−2 to 107 fM or some nine orders of magnitude (Figure 4). No observable effects were seen below 10−5 fM, and RIF-1 appears to be inactive above 108 fM. A. machipongonensis conditioned medium contains 104 fM RIF-1 (Figure 4—figure supplements 1–3), and even if this conditioned medium measurement exaggerates natural concentrations by a factor of 106, S. rosetta could still respond to its presence. The shape of the dose-response curve and the potency of RIF-1 suggest that S. rosetta perceives RIF-1 in a manner consistent with a receptor-ligand interaction, albeit a receptor of exquisite sensitivity and remarkable dynamic range. While RIF-1 is the only molecule detected with rosette-inducing activity, its maximal activity (5.6 ± 0.5% colonial cells/total cells) differs from that of the sphingolipid-enriched lipid fraction (19.2 ± 4.6% colonial cells/total cells; Figure 4). This difference may be due to delivery issues of the purified and highly hydrophobic molecule, which in nature resides in membranes and potentially in membrane vesicles. Alternatively, the full potency of RIF-1 as an inducer of colony development may require additional A. machipongonensis molecules not identified in this study.

Figure 4 with 3 supplements see all
Purified RIF-1 is active at plausible environmental concentrations.

RIF-1 concentrations ranging from 10−2 to 107 fM induce rosette colony development in RCA cultures. Frequency of rosette colony development was quantified in RCA cultures 2 days after treatment with a dilution series of purified RIF-1. Data are mean ± s.e. from three independent experiments. Line indicates non-linear regression of the RIF-1 activity profile.

https://doi.org/10.7554/eLife.00013.025

Discussion

These data reveal that RIF-1, a sulfonolipid produced by the prey bacterium A. machipongonensis, regulates morphogenesis in its predator, S. rosetta. The ecological relevance of this signaling interaction is indicated both by the coexistence of S. rosetta and A. machipongonensis in nature and by the fact that the activity of RIF-1 at femtomolar concentrations makes it markedly more potent than other marine signaling molecules [e.g., Vibrio autoinducer (Schaefer et al. 1996) and the tripeptide pheromones of the Caribbean spiny lobster (Ziegler and Forward 2007)]. The potency of RIF-1 signaling compares favorably with that of silkworm moth sex pheromone signaling, in which vapors from an ∼4 fM solution of bombykol, the sex pheromone of the silkworm moth, induce a pronounced wing fluttering response in males (Butenandt et al. 1961; Agosta 1992; Roelofs 1995). While it is formally possible that RIF-1-dependent rosette colony development is a promiscuous response to sphingolipid-type molecules, only a handful of sulfonolipids like RIF-1 have been reported (Godchaux and Leadbetter 1980, 1983, 1984, 1988; Drijber and McGill 1994; Kamiyama et al. 1995a, 1995b; Kobayashi et al. 1995) and no other A. machipongonensis lipid tested in this study induced rosette colony development. Therefore we favor a model in which A. machipongonensis cell density, as revealed by RIF-1 concentration, provides S. rosetta with an indication of conditions under which rosette colony development would be advantageous, for instance by promoting more efficient capture of planktonic bacteria (Kreft 2010). In analogy to the chemotaxis system of bacteria (Falke et al. 1997), the ability of S. rosetta to respond to increasing bacterial cell density likely requires the hypothesized RIF-1 receptor to become less sensitive at higher concentrations. The high concentration cutoff in the dose-response curve reflects a complete loss of sensitivity at high, but non-physiological, RIF-1 concentrations. Although the presence of RIF-1 in the A. machipongonensis cell envelope suggests that it may be encountered by S. rosetta during phagocytosis, it can also function at a distance. We hypothesize that RIF-1 may be released into the environment in membrane vesicles, which have been described in Gram-negative bacteria such as Bacteroidetes (Zhou et al. 1998; Møller et al. 2005), and that additional membrane constituents might be required for the full potency of RIF-1. In the future, elucidating RIF-1 delivery, along with determining the three-dimensional structure of RIF-1 and characterizing sulfonolipids from other Bacteroidetes will begin to provide the needed foundation for a molecular understanding of how S. rosetta perceives RIFs.

The morphogenetic interaction described here between S. rosetta and A. machipongonensis raises the possibility that bacterially-produced sphingolipids in general, and sulfonolipids in particular, may be essential for the chemical signaling that allows Bacteroidetes to influence cell differentiation and morphogenesis in diverse animals (Falkow 2006; Mazmanian et al. 2008; Lee and Mazmanian 2010; An et al. 2011). Sulfonolipids like RIF-1 have been reported to have therapeutic activities, but their endogenous functions are not known. Sulfobacins A and B, which were isolated from the culture broth of a Chryseobacterium sp. were reported as von Willebrand factor receptor antagonists, and flavocristamide A, from a related bacterial species, was reported as a DNA polymerase α inhibitor (Kamiyama et al. 1995a; Kobayashi et al. 1995). The pervasiveness of interactions between Bacteroidetes and animals (Webster et al. 2004; Wexler 2007), coupled with the close evolutionary relationship between choanoflagellates and animals (King and Carroll 2001; King 2004; King et al. 2008; Ruiz-Trillo et al. 2008), raise the possibility that the connection between Bacteroidetes and animal development has deep evolutionary roots (McFall-Ngai 1999). The discovery of RIF-1 and its biological activity toward S. rosetta provides both the molecular basis and model organism for further understanding a new and potentially important class of small molecule information transfer.

Materials and methods

Choanoflagellate husbandry and microscopy

Request a detailed protocol

The environmental isolate of Salpingoeca rosetta is deposited at the American Tissue Culture Collection (ATCC) under the designation ATCC50818 (King et al. 2003). The Rosette Colonies Absent (RCA) culture line was produced from ATCC50818 by serial treatment with chloramphenicol (68 μg mL−1), ampicillin (50 μg mL−1), streptomycin (50 μg mL−1), and erythromycin (50 μg mL−1) (Fairclough et al. 2010). A monoxenic line of S. rosetta (Px1) was generated by treating ATCC 50818 with a combination of ofloxacin (10 μg mL−1), kanamycin (50 μg mL−1), and streptomycin (50 μg mL−1) antibiotics to kill the undefined environmental bacteria. Following several rounds of serial dilution, a single cell was isolated by FACS and supplemented with A. machipongonensis (Dayel et al. 2011). All three S. rosetta cell lines (ATCC 50818, RCA, and Px1) were grown in cereal grass infused seawater at 25°C and maintained by splitting cultures 1:10 into fresh medium every 3 days (King et al. 2009). Live cells were imaged with a Leica DMI6000B microscope equipped with a DFC350 FX camera.

Bioassay for rosette colony development

Request a detailed protocol

Under laboratory conditions, S. rosetta differentiates into a variety of cell types including attached thecate cells, solitary swimmers, rosette colonies, chain colonies and loose, disorganized associations of cells attached to one another at the collar or to bacterial biofilms (Dayel et al. 2011). S. rosetta rosette colonies can be distinguished from other cell types in that they contain clusters of at least four closely associated cells with organized polarity; each cell oriented with its flagellum pointing outward from a central focus. In the qualitative bioassay, RCA cultures were diluted in fresh medium to a density of approximately 104–105 cells mL−1, aliquoted into 24-well flat bottom culture dishes (Costar, Corning, NY, USA), supplemented with various treatments, and scored for the presence or absence of rosette colonies after 48 hr. For quantitative measurements, RCA cultures were diluted as before into six-well flat bottom culture dishes. To measure the percentage of cells within rosette colonies, each well was scraped to detach thecate cells and the total number of cells and the total number of cells in each rosette colony were counted with a Bright-Line hemacytometer (Hausser Scientific, Horsham, PA, USA).

Isolation and identification of A. machipongonensis

Request a detailed protocol

A partial representation of the bacterial flora from ATCC50818 was isolated by standard dilution-plating technique on modified Zobell medium agar (Carlucci and Pramer 1957) at 25°C. Individual isolates were tested for their morphogenic activity by supplementing RCA cultures with a single colony of each isolate. Of 64 isolates tested, the only one that restored rosette colony development to the RCA cell line was a species that formed pink-pigmented colonies (designated strain PR1). Strain PR1 was used to inoculate liquid modified Zobell medium at 25°C and grown with aeration overnight. PR1 cells were harvested by centrifugation, and genomic DNA was isolated using a Bacterial Genomic DNA Mini-prep Kit (Bay Gene, Burlingame, CA, USA) according to the manufacturer’s specifications. The 16S rRNA gene was amplified using universal primers 8F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-ACCTTGTTACGRCTT-3′) (Weisburg et al. 1991); comparison of the PR1 16S rRNA sequence to the Greengenes 16S rRNA database (DeSantis et al. 2006) revealed strain PR1 to be most closely related to members of the Algoriphagus genus within the Bacteroidetes phylum. PR1 was subsequently named Algoriphagus machipongonensis (Bradley et al. 2009).

Generating a phylogenetic framework for testing the diversity of bacteria that induce rosette colony development

Request a detailed protocol

To investigate whether the ability to trigger rosette colony development was specific to A. machipongonensis, we tested three classes of bacterial species for their morphogenic capacity: 15 species in the Algoriphagus genus, 16 non-Algoriphagus members of the Bacteroidetes phylum, and eight species representing three additional major clades within Bacteria. Each species was screened for morphogenic activity using the bioassay for rosette colony development. Live cells from individual colonies grown from solid agar plates were added directly to RCA cultures and scored for the presence or absence of rosette colonies 48 hr after inoculation. Each bacterial species was tested three times.

To determine the phylogenetic distribution of morphogenic activity in the bacterial species tested (Table 1), a sequence alignment of 16S rDNA genes from each species was generated by iterative pairwise comparisons using FSA (Bradley et al. 2009). Poorly aligned regions were removed by Gblocks version 0.91b (Castresana 2000; Talavera and Castresana 2007) using default block parameters. A distance matrix (distance options according to the Kimura two-parameter model), including clustering with the maximum likelihood algorithm, was calculated using Phylip version 3.67 (Falenstein 1989). Support for the resulting tree topology was estimated using bootstrap analysis (1000 replicates).

Biochemical analysis of Rosette Inducing Factor (RIF-1)

Request a detailed protocol

To determine the biochemical nature of RIF-1, A. machipongonensis cell fractions and conditioned medium were subjected to a battery of treatments. The results of these tests are summarized in Table 2. Conditioned medium (CM) was generated by pelleting either choanoflagellates grown in cereal grass infused with seawater or A. machipongonensis cultures grown in seawater complete medium (Atlas 2004) and filtering the culture supernatant through a 0.22 μm pore filter (Millipore) to remove live bacteria. To test whether RIF-1 activity required live bacteria, A. machipongonensis was grown overnight at 25°C, centrifuged at 16,000×g for 1 min to pellet cells, and heated for 30 min at 80°C to kill viable bacteria. To test whether RIF-1 activity might be heat labile (e.g., a polypeptide), A. machipongonensis CM was boiled for 10 min. To test whether RIF-1 was a protein, A. machipongonensis CM was incubated with 200 μg mL−1 proteinase K (New England Biolabs, Ipswich, MA, USA) for 2 hr at 37°C. To test whether RIF-1 was a nucleic acid, 25 mL of A. machipongonensis CM was lyophilized, resuspended in 2.5 mL of water, and extracted with 100% ethanol to a final concentration of 80% (vol/vol) for 2 hr at −20 °C and the precipitate was collected by centrifugation for 30 min at 4000×g at 4°C. The precipitate was dissolved in 0.01 M PBS (containing 10 mM MgCl2 and 1 mM CaCl2) and incubated with either RNase A (100 μg mL−1; Sigma) or DNase I (100 μg mL−1; Sigma) for 2.5 hr at 37°C. To test whether RIF-1 activity was in the methanolic extract, A. machipongonensis cell pellet and CM were lyophilized and vortexed with methanol. Each suspension was centrifuged at 8000 rpm for 5 min and the methanol layer recovered and dried.

To test whether RIF-1 was a lipid, A. machipongonensis cell pellet was extracted according to the Bligh–Dyer method (Bligh and Dyer 1959). Briefly, the cell pellet was resuspended in 3 vol of 1:2 (vol/vol) CHCl3:MeOH and vortexed. One volume of CHCl3 was added, and the mixture vortexed. One volume of distilled water was then added, and the mixture vortexed. The same was then centrifuged at 1000 rpm for 5 min and the bottom layer recovered and dried.

To test whether RIF-1 was a component of lipopolysaccharide (LPS), A. machipongonensis LPS was isolated using a method from Apicella (2008). Lyophilized A. machipongonensis cells were ground with a mortar and pestle and suspended in 10 mM Tris–Cl buffer (pH 8.0), containing 2% sodium dodecyl sulfate (SDS), 4% 2-mercaptoethanol, and 2 mM MgCl2. The mixture was vortexed and incubated at 65°C until solubilized. Proteinase K (20 mg mL−1) was added to the mixture, and incubated at 65°C for an additional hour, followed by 37°C incubation overnight. 3 M sodium acetate was then added and the sample mixed. Following addition of cold absolute ethanol to the cell suspension, the sample was incubated overnight at –20°C to allow precipitate to form. The mixture was centrifuged at 4000×g for 15 min, and the supernatant discarded. The precipitate was suspended in distilled water. 3 M sodium acetate was added, and the mixture vortexed. Following addition of cold absolute ethanol, the mixture was vortexed again, and the suspension again allowed to precipitate overnight at –20°C. After the centrifugation, the precipitate was suspended in 10 mM Tris–Cl (pH 7.4), and DNase I (100 μg mL−1; NEB) and RNase (25 μg mL−1; NEB) added. The mixture was incubated at 37°C for 4 hr, then placed in a 65°C water bath for 30 min. Ninety percent phenol preheated to 65°C was added, and allow to set at 65°C for 15 min. The mixture was placed in an ice bath to cool, and then centrifuged at 6000×g for 15 min. The top aqueous layer was removed, and the phenolic layer re-extracted with an equal volume of distilled water. This sample was again incubated at 65°C for 15 min and then placed in ice water. After centrifugation at 6000×g for 15 min, the two aqueous layers were combined and dialyzed against multiple changes of distilled water over 2 days.

To test whether RIF-1 was a peptidoglycan, A. machipongonensis peptidoglycan was isolated using a method adapted from de Jonge et al. (1992), A. machipongonensis cell pellet was washed with 0.8% NaCl. The cells were resuspended in hot 4% SDS, boiled for 30 min, and then incubated at room temperate overnight. The sample was boiled for an additional 10 min and then centrifuged at 15,000×g for 15 min at room temperature. The pellet was washed four times with water and resuspended in water. The sample was digested for mutanolysin (10 μg mL−1; Sigma) overnight at 37°C. The enzyme was inactivated by incubation at 80°C for 20 min.

Isolation and purification of RIF-1 from A. machipongonensis

Request a detailed protocol

A. machipongonensis was cultured in seawater complete medium (16×1 L) at 30°C for 2 days. The cells were harvested by centrifugation and extracted with CHCl3:MeOH (2:1, 4 L). The organic extract was filtered, dried over sodium sulfate (Na2SO4), and concentrated to give approximately 4 g crude lipid extract. The crude extract was dissolved in a minimum amount of CHCl3:MeOH (2:1), and purified by preparative high performance liquid chromatography (HPLC). All solvents were purchased from Fisher Scientific unless otherwise noted. Preparative reversed phase HPLC (RP-HPLC) was performed on an Agilent Technologies 1200 Series HPLC using a Phenomenex Luna 5 µm C8(2) 100 Å 250×21.2 mm column. Isolation of RIF-1 continued with a crude fractionation in which compounds were eluted at 10 mL min−1 in a gradient of solvents A (0.1% NH4OH in water) and B (0.1% NH4OH in methanol): 65% B increasing to 100% B over 30 min, isocratic at 100% B for 1 min. before returning to 65% B and re-equilibrating over 10 min. Fractions were analyzed by low-resolution mass spectrometry (LC-MS) on an Agilent 6130 LC/MS using a Phenomenex Gemini-NX 5 µm C18 110 Å 100×2 mm column. The next stage involved a higher resolution separation in which compounds were eluted at 0.5 mL min−1 in a gradient of solvents A (0.1% NH4OH in water) and B (0.1% NH4OH in methanol): 65% B increasing to 100% B over 30 min, isocratic at 100% B for 1 min before returning to 65% B and re-equilibrating over 3 min and those which contained a mass peak corresponding to RIF-1 ([M-H]=606.4) were combined and concentrated. This material was then purified by preparative TLC (1 mm, silica gel 60), eluted with CHCl3:MeOH:AcOH:H2O (100:20:12:5, Rf=0.5). RIF-1 was visualized by staining with ammonium molybdate in 10% H2SO4. The portion of the plate (Fraction F; Figure 3—figure supplement 1) that induced colony formation and contained RIF-1 (LC/MS: [M-H] 606) was scraped off after RIF-1 was visualized by staining with ammonium molybdate in 10% H2SO4, and the silica was extracted with CHCl3:MeOH (5:1). This material was further purified by preparative TLC on a 250 μm TLC plate (silica gel 60), eluted with CHCl3:MeOH:AcOH:H2O (100:20:12:5). From 16 L of A. machipongonensis culture, approximately 50 μg RIF-1 was obtained in sufficient purity. The entire process, from growth of the cells to isolation of pure RIF-1, was repeated nine times in order to obtain approximately 0.7 mg RIF-1 from a total of 160 L of A. machipongonensis culture.

High Resolution Mass Spectrometry (HRMS) was carried out by Ted Voss at the WM Keck Foundation Biotechnology Resource Laboratory at Yale University on a Bruker 9.4T FT-ICR MS. RIF-1 was dissolved in 200 μL DMSO-d6 and transferred into a 3 mm NMR tube. 1H, TOCSY, gCOSY and dqfCOSY were recorded on a Varian Inova 600 spectrometer. HMQC and gHMBC experiments were performed on a Bruker Advance (sgu) 900 MHz and Varian Unity Inova 600 MHz equipped with a cryoprobe, respectively. Chemical shifts are reported in ppm from tetramethylsilane with the solvent resonance resulting from incomplete deuteration as the internal standard (DMSO: δ 2.50). Data are reported in Table 3 as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, br = broad, m = multiplet), coupling constants, and integration. Optical rotation was measured on a Jasco P-2000 digital polarimeter with a sodium lamp at 21.4°C. Unless otherwise noted, all solvents and reagents were purchased from VWR or Fisher and used without further purification.

1H NMR (600 MHz, DMSO) and 13C NMR (600 MHz, DMSO): see Table 3. Optical rotation: [α]D21.4 +6.4 (c=0.07, MeOH). HRMS m/z calcd for C32H64NO7S (M-H): 606.44035. Found: 606.44027 (M-H). MS/MS analysis: A major fragment derived from m/z=606 (M-H) in the MS/MS spectrum of RIF-1 corresponds to amino-sulfonic acid, Figure 3—figure supplement 2. HRMS/MS m/z calculated for C17H36NO5S (M-H): 366.23142. Found: 366.2310 (M-H).

Quantification of RIF-1 levels in conditioned medium

Request a detailed protocol

Conditioned medium was prepared from A. machipongonensis grown in seawater complete medium (750 mL) at 30°C for 2 days. The conditioned medium was lyophilized and extracted with CHCl3:MeOH (2:1; 78 mL). The organic extract was filtered, further extracted with CHCl3 (60 mL×2), and filtrates were combined and concentrated to dryness under vacuum. The crude extract was suspended in 5 mL 50% MeOH:H2O and was passed through a C-18 SPE (1 g) column. The open column was then washed with 10 mL 90% MeOH:CHCl3. The organic eluate was concentrated and dissolved in 3 mL CHCl3:MeOH (2:1) for LC/MS analysis. The chromatography was carried out using an Agilent 6130 Quadrupole LC/MS system with a C18 reverse-phase column (4.6×100 mm; Phenomenex Luna; 5 μ) for 30 min in a linear gradient from solvent A (60% methanol/water with 0.1% ammonium hydroxide) to solvent B (100% methanol with 0.1% ammonium hydroxide). The RIF-1 was detected in the conditioned medium at a concentration of 80 ng L−1. The purified RIF-1 was used as the standard (Figure 4—figure supplements 1–3).

Activity profile of RIF-1

Request a detailed protocol

The potency of pure RIF-1 was determined using the quantitative bioassay for rosette colony development. Briefly, 100 ug of pure RIF-1 was solubilized in 100 μL DMSO and this 1 g L−1 stock was stored at -80°C. For each experiment, serial dilutions ranging from 10−1 g L−1 down to 10−17 g L−1 were made in DMSO. 2 μL of each dilution was premixed with 1 mL of fresh cereal grass infused seawater (King et al. 2003) to avoid precipitation of RIF-1 and the premixed RIF-1 dilution was then added to 1 mL RCA cultures to yield final concentrations ranging from 10−3 to 10−20 g L−1, equivalent to 1.6×109 fM down to 1.6×10−8 fM. The percentage of rosette colonial cells was determined as described above in three independent cell lines in triplicate. From the percent rosette colony development, a bell-shaped dose-response model was determined to be the nonlinear regression curve of best fit determined using GraphPad Prism 5 statistical software.

References

  1. Book
    1. Agosta WC
    (1992)
    Chemical communication
    New York: Scientific American Press.
    1. Ahmed I
    2. Yokota A
    3. Fujiwara T
    (2007)
    Chimaereicella boritolerans sp nov., a boron-tolerant and alkaliphilic bacterium of the family Flavobacteriaceae isolated from soil
    Int J Syst Evol Microbiol 57:986–992.
    1. Alegado RA
    2. Grabenstatter JD
    3. Zuzow R
    4. Morris A
    5. Huang SY
    6. Summons RE
    7. King N
    (2012)
    Algoriphagus machipongonensis sp. nov. co-isolated with a colonial choanoflagellate
    Int J Syst Evol Microbiol, [epub ahead of print].
    1. An D
    2. Na C
    3. Bielawski J
    4. Hannun YA
    5. Kasper DL
    (2011)
    Membrane sphingolipids as essential molecular signals for Bacteroides survival in the intestine
    Proc Natl Acad Sci U S A 1:4666–4671.
    1. Anton J
    2. Oren A
    3. Benlloch S
    4. Rodriguez-Valera F
    5. Amann R
    6. Rossello-Mora R
    (2002)
    Salinibacter ruber gen. nov., sp nov., a novel, extremely halophilic member of the bacteria from saltern crystallizer ponds
    Int J Syst Evol Microbiol 52:485–491.
    1. Apicella MA
    (2008)
    Isolation and characterization of lipopolysaccharides
    Methods Mol Biol 431:3–13.
    1. Atlas RM
    (2004)
    Handbook of microbiological media
    2051, Handbook of microbiological media, p., Boca Raton, CRC.
    1. Barbeyron T
    2. L’Haridon S
    3. Corre E
    4. Kloareg B
    5. Potin P
    (2001)
    Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov
    Int J Syst Evol Microbiol 51:985–997.
    1. Bernardet J
    2. Segers P
    3. Vancanneyt M
    4. Berthe F
    5. Kersters K
    6. Vandamme P
    (1996)
    Cutting a Gordian knot: Emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom nov (basonym, Cytophaga aquatilis Strohl and Tait 1978)
    Int J Syst Bacteriol 46:128–148.
    1. Blattner FR
    2. Plankett G III
    3. Bloch CA
    4. Perna N
    5. Burland V
    6. Riley M
    7. Collado-Vides J
    8. Glasner JD
    9. Rode CK
    10. Mayhew GF
    et al. (1997)
    The complete genome sequence of Escherichia coli K-12
    Science 277:1453–1462.
    1. Bligh EG
    2. Dyer WJ
    (1959)
    A rapid method of total lipid extraction and purification
    Can J Biochem Physiol 37:911–917.
    1. Bowman JP
    2. Nichols CM
    3. Gibson JAE
    (2003)
    Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats
    Int J Syst Evol Microbiol 53:1343–1355.
    1. Bradley RK
    2. Roberts A
    3. Smoot M
    4. Juvekar S
    5. Do J
    6. Dewey C
    7. Holmes I
    8. Pachter L
    (2009)
    Fast statistical alignment
    PLoS Comput Biol 5:e1000392.
    1. Brettar I
    2. Christen R
    3. Hofle M
    (2004a)
    Aquiflexum balticum gen. nov., sp nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea
    Int J Syst Evol Microbiol 54:2335–2341.
    1. Brettar I
    2. Christen R
    3. Hofle MG
    (2004b)
    Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea
    Int J Syst Evol Microbiol 54:65–70.
    1. Burkholder PR
    2. Giles NH
    (1947)
    Induced biochemical mutations in Bacillus subtilis
    Am J Bot 34:345–348.
    1. Butenandt A
    2. Beckmann R
    3. Hecker E
    (1961)
    On the sex attractant of silk-moths. I. The biological test and the isolation of the pure sex-attractant bombykol
    Hoppe-Seylers Z Physiol Chem 324:71–83.
    1. Carlucci AF
    2. Pramer D
    (1957)
    Factors influencing the plate method for determining abundance of bacteria in sea water
    Proc Soc Exp Biol Med 96:392–394.
    1. Carr M
    2. Leadbeater BSC
    3. Hassan R
    4. Nelson M
    5. Baldauf SL
    (2008)
    Molecular phylogeny of choanoflagellates, the sister group to Metazoa
    Proc Natl Acad Sci U S A 105:16641–16646.
    1. Castresana J
    (2000)
    Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis
    Mol Biol Evol 17:540–552.
    1. Cerdeno-Tarraga AM
    2. Patrick S
    3. Crossman L
    4. Blakely GW
    5. Abratt V
    6. Lennard N
    7. Poxton I
    8. Duerden B
    9. Harris B
    10. Quail MA
    et al. (2005)
    Extensive DNA inversions in the B. fragilis genome control variable gene expression.
    Science 307:1463–1465.
    1. Chelius M
    2. Triplett E
    (2000)
    Dyadobacter fermentans gen. nov., sp nov., a novel gram-negative bacterium isolated from surface-sterilized Zea mays stems
    Int J Syst Evol Microbiol 50:751–758.
    1. Cho J-C
    2. Giovannoni SJ
    (2003)
    Croceibacter atlanticus gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae
    Syst Appl Microbiol 26:76–83.
    1. Cho J-C
    2. Giovannoni SJ
    (2004)
    Robiginitalea biformata gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae with a higher G+C content
    Int J Syst Evol Microbiol 54:1101–1106.
    1. Cohn ZA
    2. Morse SI
    (1960)
    Functional and metabolic properties of polymorphonuclear leucocytes. II. The influence of a lipopolysaccharide endotoxin
    J Exp Med 111:689–704.
    1. Dayel MJ
    2. Alegado RA
    3. Fairclough SR
    4. Levin TC
    5. Nichols SA
    6. McDonald K
    7. King N
    (2011)
    Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta
    Dev Biol 357:73–82.
    1. DeSantis TZ
    2. Hugenholtz P
    3. Larsen N
    4. Rojas M
    5. Brodie EL
    6. Keller K
    7. Huber T
    8. Dalevi D
    9. Hu P
    10. Andersen GL
    (2006)
    Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB
    Appl Environ Microbiol 72:5069–5072.
    1. Desbrosses GJ
    2. Stougaard J
    (2011)
    Root nodulation: A paradigm for how plant-microbe symbiosis influences host developmental pathways
    Cell Host Microbe 10:348–358.
    1. Drijber R
    2. McGill W
    (1994)
    Sulfonolipid content of Cytophaga and Flexibacter species isolated from soil and cultured under different nutrient and temperature regimes
    Can J Microbiol 40:132–139.
    1. Edman DC
    2. Pollock MB
    3. Hall ER
    (1968)
    Listeria monocytogenes L forms. I. Induction maintenance, and biological characteristics
    J Bacteriol 96:352–357.
    1. Fairclough SR
    2. Dayel MJ
    3. King N
    (2010)
    Multicellular development in a choanoflagellate
    Curr Biol 20:R875–R876.
    1. Falenstein J
    (1989)
    PHYLIP—Phylogeny inference packages (version 3.2)
    Cladistics 5:164–166.
    1. Falke JJ
    2. Bass RB
    3. Butler SL
    4. Chervitz SA
    5. Danielson MA
    (1997)
    The two-component signaling pathway of bacterial chemotaxis: A molecular view of signal transduction by receptors, kinases, and adaptation enzymes
    Annu Rev Cell Dev Biol 13:457–512.
    1. Falkow S
    (2006)
    Is persistent bacterial infection good for your health?
    Cell 124:699–702.
    1. Garrity GM
    (2010)
    Bergey’s manual of systematic bacteriology: Bacteroidetes, spirochetes, tenericutes (mollicutes), acidobacteria, fibrobacteres, fusobacteria, dictyoglomi, gemmatimonadetes, lentisphaerae, verrucomicrobia, chlamydiae, and planctomycetes
    949, Bergey’s manual of systematic bacteriology: Bacteroidetes, spirochetes, tenericutes (mollicutes), acidobacteria, fibrobacteres, fusobacteria, dictyoglomi, gemmatimonadetes, lentisphaerae, verrucomicrobia, chlamydiae, and planctomycetes, p., New York, Springer Verlag.
    1. Godchaux W
    2. Leadbetter ER
    (1980)
    Capnocytophaga spp. contain sulfonolipids that are novel in procaryotes
    J Bacteriol 144:592–602.
    1. Godchaux W
    2. Leadbetter ER
    (1983)
    Unusual sulfonolipids are characteristic of the Cytophaga-Flexibacter group
    J Bacteriol 153:1238–1246.
    1. Godchaux W
    2. Leadbetter ER
    (1984)
    Sulfonolipids of gliding bacteria. Structure of the N-acylaminosulfonates
    J Biol Chem 259:2982–2990.
    1. Godchaux W
    2. Leadbetter E
    (1988)
    Sulfonolipids are localized in the outer membrane of the gliding bacterium Cytophaga johnsonae
    Arch Microbiol 150:42–47.
    1. Hannun YA
    2. Luberto C
    3. Argraves KM
    (2001)
    Enzymes of sphingolipid metabolism: From modular to integrative signaling
    Biochemistry 40:4893–4903.
    1. Herr DR
    2. Fyrst H
    3. Phan V
    4. Heinecke K
    5. Georges R
    6. Harris GL
    7. Saba JD
    (2003)
    Sply regulation of sphingolipid signaling molecules is essential for Drosophila development
    Development 130:2443–2453.
    1. Hibberd DJ
    (1975)
    Observations on the ultrastructure of the choanoflagellate Codosiga botrytis (Ehr.) Saville-Kent with special reference to the flagellar apparatus
    J Cell Sci 17:191–219.
    1. Hoffmann JA
    2. Kafatos FC
    3. Janeway CA
    4. Ezekowitz RA
    (1999)
    Phylogenetic perspectives in innate immunity
    Science 284:1313–1318.
    1. Hughes DT
    2. Sperandio V
    (2008)
    Inter-kingdom signalling: Communication between bacteria and their hosts
    Nat Rev Microbiol 6:111–120.
    1. James-Clark H
    (1867)
    On the spongiae ciliatae as infusoria flagellata; or observations on the structure, animality, and relationship of Leucosolenia botryoides, Bowerbank.
    Memoirs Boston Soc. Of Nat. Hist.
    1. de Jonge BL
    2. Chang YS
    3. Gage D
    4. Tomasz A
    (1992)
    Peptidoglycan composition in heterogeneous Tn551 mutants of a methicillin-resistant Staphylococcus aureus strain
    J Biol Chem 267:11255–11259.
    1. Kamiyama T
    2. Umino T
    3. Itezono Y
    4. Nakamura Y
    5. Satoh T
    6. Yokose K
    (1995a)
    Sulfobacins A and B, novel von Willebrand factor receptor antagonists. II. Structural elucidation
    J Antibiot 48:929–936.
    1. Kamiyama T
    2. Umino T
    3. Satoh T
    4. Sawairi S
    5. Shirane M
    6. Ohshima S
    7. Yokose K
    (1995b)
    Sulfobacins A and B, novel von Willebrand factor receptor antagonists. I. Production, isolation, characterization and biological activities
    J. Antibiot 48:924–928.
    1. Karpov SA
    2. Coupe SJ
    (1998)
    A revision of choanoflagellate genera Kentrosiga, Schiller, 1953 and Desmarella, Kent, 1880
    Acta Protozoologica 37:23–27.
    1. Kimbrell DA
    2. Beutler B
    (2001)
    The evolution and genetics of innate immunity
    Nat Rev Genet 2:256–267.
    1. King N
    (2004)
    The unicellular ancestry of animal development
    Dev Cell 7:313–325.
    1. King N
    2. Carroll SB
    (2001)
    A receptor tyrosine kinase from choanoflagellates: Molecular insights into early animal evolution
    Proc Natl Acad Sci U S A 98:15032–15037.
    1. King N
    2. Hittinger CT
    3. Carroll SB
    (2003)
    Evolution of key cell signaling and adhesion protein families predates animal origins
    Science 301:361–363.
    1. King N
    2. Westbrook MJ
    3. Young SL
    4. Kuo A
    5. Abedin M
    6. Chapman J
    7. Fairclough S
    8. Hellsten U
    9. Isogai Y
    et al. (2008)
    The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans
    Nature 451:783–788.
    1. King N
    2. Young SL
    3. Abedin M
    4. Carr M
    5. Leadbeater BSC
    (2009)
    Starting and maintaining Monosiga brevicollis cultures
    Cold Spring Harb Protoc, 2009: pdb.prot5148.
    1. Kobayashi J
    2. Mikami S
    3. Shigemori H
    4. Takao TYS
    5. Izuta S
    6. Yoshida S
    (1995)
    Flavocristamides A and B, new DNA polymerase α inhibitors from a marine bacterium sp
    Tetrahedron 51:10487–10490.
    1. Kopp EB
    2. Medzhitov R
    (1999)
    The Toll-receptor family and control of innate immunity
    Curr Opin Immunol 11:13–18.
    1. Koropatnick TA
    2. Engle JT
    3. Apicella MA
    4. Stabb EV
    5. Goldman WE
    6. Mcfall-Ngai MJ
    (2004)
    Microbial factor-mediated development in a host-bacterial mutualism
    Science 306:1186–1188.
    1. Kreft JM
    (2010)
    Thesis (M.A. in Integrative Biology)
    Thesis (M.A. in Integrative Biology), University of California, Berkeley.
    1. Kunst F
    2. Ogasawara N
    3. Moszer I
    4. Albertini AM
    5. Alloni G
    6. Azevedo V
    7. Bertero MG
    8. Bessières P
    9. Bolotin A
    10. Borchert S
    et al. (1997)
    The complete genome sequence of the gram-positive bacterium Bacillus subtilis
    Nature 390:249–256.
    1. Leadbeater BSC
    (1983)
    Life-history and ultrastructure of a new marine species of Proterospongia (Choanoflagellida)
    J Mar Biol Assoc UK 63:135–160.
    1. Lee YK
    2. Mazmanian SK
    (2010)
    Has the microbiota played a critical role in the evolution of the adaptive immune system?
    Science 330:1768–1773.
    1. Lewin R
    (1969)
    A classification of flexibacteria
    J Gen Microbiol 58:189–206.
    1. Matsunaga T
    2. Okamura Y
    3. Fukuda Y
    4. Wahyudi AT
    5. Murase Y
    6. Takeyama H
    (2005)
    Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1
    DNA Res 12:157–166.
    1. Matsuo Y
    2. Suzuki M
    3. Kasai H
    4. Shizuri Y
    5. Harayama S
    (2003)
    Isolation and phylogenetic characterization of bacteria capable of inducing differentiation in the green alga Monostroma oxyspermum
    Environ Microbiol 5:25–35.
    1. Matsuo Y
    2. Imagawa H
    3. Nishizawa M
    4. Shizuri Y
    (2005)
    Isolation of an algal morphogenesis inducer from a marine bacterium
    Science 307:1598.
    1. Mazmanian SK
    2. Liu CH
    3. Tzianabos AO
    4. Kasper DL
    (2005)
    An immunomodulatory molecule of symbiotic bacteria directs maturation of the host immune system
    Cell 122:107–118.
    1. Mazmanian SK
    2. Round JL
    3. Kasper DL
    (2008)
    A microbial symbiosis factor prevents intestinal inflammatory disease
    Nature 453:620–625.
    1. McFall-Ngai M
    (1999)
    Consequences of evolving with bacterial symbionts: Insights from the squid-vibrio associations
    Ann Rev Ecol Syst 30:235–256.
    1. Medzhitov R
    2. Janeway C
    (2000)
    Innate immune recognition: Mechanisms and pathways
    Immunol Rev 173:89–97.
    1. Merrill AH
    2. Sullards MC
    3. Wang E
    4. Voss KA
    5. Riley RT
    (2001)
    Sphingolipid metabolism: Roles in signal transduction and disruption by fumonisins
    Environ Health Perspect 2:283–289.
    1. Miller TR
    2. Delcher AL
    3. Salzberg SL
    4. Saunders E
    5. Detter JC
    6. Halden RU
    (2010)
    Genome sequence of the dioxin-mineralizing bacterium Sphingomonas wittichii RW1
    J Bacteriol 192:6101–6102.
    1. Møller JD
    2. Barnes AC
    3. Dalsgaard I
    4. Ellis AE
    (2005)
    Characterisation of surface blebbing and membrane vesicles produced by Flavobacterium psychrophilum
    Dis Aquat Organ 64:201–209.
    1. Nedashkovskaya OI
    2. Vancanneyt M
    3. Van Trappen S
    4. Vandemeulebroecke K
    5. Lysenko AM
    6. Rohde M
    7. Falsen E
    8. Frolova GM
    9. Mikhailov VV
    10. Swings J
    et al. (2004)
    Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman,
    Int J Syst Evol Microbiol 54:1757–1764.
    1. Nedashkovskaya O
    2. Kim S
    3. Vancanneyt M
    4. Lysenko A
    5. Shin D
    6. Park M
    7. Lee KH
    8. Jung WJ
    9. Kalinovskaya NI
    10. Mikhailov VV
    et al. (2006)
    Echinicola pacifica gen. nov., sp nov., a novel flexibacterium isolated from the sea urchin Strongylocentrotus intermedius
    Int J Syst Evol Microbiol 56:953–958.
    1. Nierman WC
    2. Feldblyum TV
    3. Laub MT
    4. Paulsen IT
    5. Nelson KE
    6. Eisen JA
    7. Heidelberg JF
    8. Alley MR
    9. Ohta N
    10. Maddock JR
    et al. (2001)
    Complete genome sequence of Caulobacter crescentus
    Proc Natl Acad Sci U S A 98:4136–4141.
    1. Oh H-M
    2. Kang I
    3. Yang S-J
    4. Jang Y
    5. Vergin KL
    6. Giovannoni SJ
    7. Cho JC
    (2011)
    Complete genome sequence of strain HTCC2170, a novel member of the genus Maribacter in the family Flavobacteriaceae
    J Bacteriol 193:303–304.
    1. Olsen I
    2. Jantzen E
    (2001)
    Sphingolipids in bacteria and fungi
    Anaerobe 7:103–112.
    1. Prieschl EE
    2. Baumruker T
    (2000)
    Sphingolipids: Second messengers, mediators and raft constituents in signaling
    Immunol Today 21:555–560.
    1. Provasoli L
    2. Pintner I
    (1980)
    Bacteria induced polymorphism in an axenic laboratory strain of Ulva lactuca (Chlorophyceae)
    J Phycol 16:196–201.
    1. Pyne S
    2. Pyne NJ
    (2000)
    Sphingosine 1-phosphate signalling in mammalian cells
    Biochem J 349:385–402.
    1. Raj H
    2. Maloy S
    (1990)
    Proposal of Cyclobacterium-Marinus gen-nov, comb-nov for a marine bacterium previously assigned to the genus Flectobacillus
    Int J Syst Bacteriol 40:337–347.
    1. Roelofs WL
    (1995)
    Chemistry of sex attraction
    Proc Natl Acad Sci U S A 92:44–49.
    1. Ruby EG
    2. Urbanowski M
    3. Campbell J
    4. Dunn A
    5. Faini M
    6. Gunsalus R
    7. Lostroh P
    8. Lupp C
    9. McCann J
    10. Millikan D
    et al. (2005)
    Complete genome sequence of Vibrio fischeri: A symbiotic bacterium with pathogenic congeners
    Proc Natl Acad Sci U S A 102:3004–3009.
    1. Ruiz-Trillo I
    2. Roger AJ
    3. Burger G
    4. Gray MW
    5. Lang BF
    (2008)
    A phylogenomic investigation into the origin of metazoa
    Mol Biol Evol 25:664–672.
  2. Book
    1. Saville Kent W
    (1880)
    A manual of the infusoria
    London: David Bogue.
    1. Schaefer AL
    2. Hanzelka BL
    3. Eberhard A
    4. Greenberg EP
    (1996)
    Quorum sensing in Vibrio fischeri: Probing autoinducer-LuxR interactions with autoinducer analogs
    J Bacteriol 178:2897–2901.
    1. Spiegel S
    2. Milstien S
    (2000)
    Sphingosine-1-phosphate: Signaling inside and out
    FEBS Lett 476:55–57.
    1. Spizizen J
    (1958)
    Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate
    Proc Natl Acad Sci USA 44:1072–1078.
    1. Takeuchi O
    2. Hoshino K
    3. Kawai T
    4. Sanjo H
    5. Takada H
    6. Ogawa T
    7. Takeda K
    8. Akira S
    (1999)
    Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components
    Immunity 11:443–451.
    1. Talavera G
    2. Castresana J
    (2007)
    Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments
    Syst Biol 56:564–577.
    1. Tiago I
    2. Mendes V
    3. Pires C
    4. Morais PV
    5. Veríssimo A
    (2006)
    Chimaereicella alkaliphila gen. nov., sp. nov., a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater
    Syst Appl Microbiol 29:100–108.
    1. Van Trappen S
    2. Vandecandelaere I
    3. Mergaert J
    4. Swings J
    (2004)
    Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes
    Int J Syst Evol Microbiol 54:1969–1973.
    1. Webster NS
    2. Smith LD
    3. Heyward AJ
    4. Watts JEM
    5. Webb RI
    6. Blackall LL
    7. Negri AP
    (2004)
    Metamorphosis of a scleractinian coral in response to microbial biofilms
    Appl Environ Microbiol 70:1213–1221.
    1. Weisburg WG
    2. Barns SM
    3. Pelletier DA
    4. Lane DJ
    (1991)
    16S ribosomal DNA amplification for phylogenetic study
    J Bacteriol 173:697–703.
    1. Wexler HM
    (2007)
    Bacteroides: The good, the bad, and the nitty-gritty
    Clin Microbiol Rev 20:593–621.
    1. Wood DW
    2. Setubal JC
    3. Kaul R
    4. Monks DE
    5. Kitajima JP
    6. Okura VK
    7. Zhou Y
    8. Chen L
    9. Wood GE
    10. Almeida NF Jr
    et al. (2001)
    The genome of the natural genetic engineer Agrobacterium tumefaciens C58
    Science 294:2317–2323.
    1. Yi H
    2. Chun J
    (2004)
    Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment
    Int J Syst Evol Microbiol 54:157–162.
    1. Yoon J-H
    2. Yeo S-H
    3. Oh TK
    (2004)
    Hongiella marincola sp. nov., isolated from sea water of the East Sea in Korea
    Int J Syst Evol Microbiol 54:1845–1848.
    1. Yoon J-H
    2. Kang S-J
    3. Oh TK
    (2005a)
    Algoriphagus locisalis sp. nov., isolated from a marine solar saltern
    Int J Syst Evol Microbiol 55:1635–1639.
    1. Yoon J-H
    2. Kang S-J
    3. Jung S-Y
    4. Lee C-H
    5. Oh TK
    (2005b)
    Algoriphagus yeomjeoni sp. nov., isolated from a marine solar saltern in the Yellow Sea, Korea
    Int J Syst Evol Microbiol 55:865–870.
    1. Yoon J-H
    2. Lee M-H
    3. Kang S-J
    4. Oh TK
    (2006)
    Algoriphagus terrigena sp. nov., isolated from soil
    Int J Syst Evol Microbiol 56:777–780.
    1. Zhou L
    2. Srisatjaluk R
    3. Justus DE
    4. Doyle RJ
    (1998)
    On the origin of membrane vesicles in gram-negative bacteria
    FEMS Microbiol Lett 163:223–228.
    1. Ziegler TA
    2. Forward RB
    (2007)
    Larval release behaviors in the Caribbean spiny lobster, Panulirus argus: Role of peptide pheromones
    J Chem Ecol 33:1795–1805.

Article and author information

Author details

  1. Rosanna A Alegado

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    Contribution
    RA: Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Contributed equally with
    Laura W Brown
    Competing interests
    No competing interests declared.
  2. Laura W Brown

    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States
    Contribution
    LB: Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Contributed equally with
    Rosanna A Alegado
    Competing interests
    No competing interests declared.
  3. Shugeng Cao

    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States
    Contribution
    SC: Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    No competing interests declared.
  4. Renee K Dermenjian

    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States
    Contribution
    RD: Acquisition of data, Analysis and interpretation of data
    Competing interests
    No competing interests declared.
  5. Richard Zuzow

    Department of Biochemistry, Stanford University School of Medicine, Stanford, United States
    Contribution
    RZ: Acquisition of data, Analysis and interpretation of data
    Competing interests
    No competing interests declared.
  6. Stephen R Fairclough

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    Contribution
    SF: Acquisition of data, Analysis and interpretation of data
    Competing interests
    No competing interests declared.
  7. Jon Clardy

    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States
    Contribution
    JC: Conception and design, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    jon_clardy@hms.harvard.edu
    Competing interests
    JC: Reviewing Editor, eLife.
  8. Nicole King

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    Contribution
    NK: Conception and design, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    nking@berkeley.edu
    Competing interests
    No competing interests declared.

Funding

Gordon and Betty Moore Foundation Marine Microbiology Initiative

  • Nicole King

National Institutes of Health (F32 GM086054)

  • Rosanna A. Alegado

National Institutes of Health (F32 GM089018)

  • Laura W. Brown

National Institutes of Health (R01 GM086258)

  • Jon Clardy

National Institutes of Health (R01 GM099533)

  • Jon Clardy
  • Nicole King

National Institutes of Health (T32 HG00047)

  • Stephen R. Fairclough

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Michael Fischbach, Richard Losick, and Russell Vance for critical reading of the manuscript. NK is a Fellow in the Integrated Microbial Biodiversity Program of the Canadian Institute for Advanced Research.

Copyright

© 2012, Alegado et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Rosanna A Alegado
  2. Laura W Brown
  3. Shugeng Cao
  4. Renee K Dermenjian
  5. Richard Zuzow
  6. Stephen R Fairclough
  7. Jon Clardy
  8. Nicole King
(2012)
A bacterial sulfonolipid triggers multicellular development in the closest living relatives of animals
eLife 1:e00013.
https://doi.org/10.7554/eLife.00013

Share this article

https://doi.org/10.7554/eLife.00013

Further reading

  1. Bacteria might have influenced the origin of multicellularity in animals.

    1. Cell Biology
    Satoshi Ninagawa, Masaki Matsuo ... Kazutoshi Mori
    Research Advance

    How the fate (folding versus degradation) of glycoproteins is determined in the endoplasmic reticulum (ER) is an intriguing question. Monoglucosylated glycoproteins are recognized by lectin chaperones to facilitate their folding, whereas glycoproteins exposing well-trimmed mannoses are subjected to glycoprotein ER-associated degradation (gpERAD); we have elucidated how mannoses are sequentially trimmed by EDEM family members (George et al., 2020; 2021 eLife). Although reglucosylation by UGGT was previously reported to have no effect on substrate degradation, here we directly tested this notion using cells with genetically disrupted UGGT1/2. Strikingly, the results showed that UGGT1 delayed the degradation of misfolded substrates and unstable glycoproteins including ATF6α. An experiment with a point mutant of UGGT1 indicated that the glucosylation activity of UGGT1 was required for the inhibition of early glycoprotein degradation. These and overexpression-based competition experiments suggested that the fate of glycoproteins is determined by a tug-of-war between structure formation by UGGT1 and degradation by EDEMs. We further demonstrated the physiological importance of UGGT1, since ATF6α cannot function properly without UGGT1. Thus, our work strongly suggests that UGGT1 is a central factor in ER protein quality control via the regulation of both glycoprotein folding and degradation.